Bio-Scale™ Mini Affi-Prep® Protein A Resin Cartridges

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Overview

Bio-Scale Mini Affi-Prep Protein A Cartridges are 1 ml and 5 ml cartridges of Affi-Prep Protein A Resin. Affinity chromatography on this support yields highly purified immunoglobulins (IgG).
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Description

Description

Bio-Scale Mini Affi-Prep protein A cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with Affi-Prep protein A support. Affinity chromatography on this support yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. Advantages include:

  • High purity of IgG
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with optimized MAPS buffer solutions

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

* U.S. patent 7,208,087.

Specifications

Specifications

Sizes
1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate
Varies for each support type*
Maximum operating pressure
1, 5, and 10 ml: 45 psi
Fittings
Female luer inlet and male luer outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Dimensions
1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.
 

Ordering

Ordering

items

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7324600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4602_tn.jpg
7324602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4620_tn.jpg
7324660

Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

732-4624_tn.jpg
7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

Documents

Documents

Number Description Options
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
5726
Profinia Protein A and G methods Product Information Sheet, Rev A
5712
The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B