Droplet Digital PCR for Gene Expression Profiling
Quantitative PCR for Gene Expression Analysis
Rapid and Cost Effective
Real-time PCR has become the tool of choice for its rapid and sensitive detection of nucleic acids across a wide dynamic range, from biological samples. Real-time PCR can be qualitative (determining the presence or absence of a gene sequence) or quantitative (copy number variation of an expressed gene). Real-time PCR that is quantitative, or qPCR, is very popular for gene expression analysis.
In RT-qPCR, the amplification reaction is set up with PCR reagents, primers and probes in a master mix, and run using real time PCR instruments. The target specific fluorescence from the amplification reaction is monitored in real time and is proportional to the amount of amplified product.
Quantifying the absolute amount of a target sequence or comparing relative amounts of a target sequence between samples uses the Cq value and is calculated by your cycler's proprietary software. This value determines the PCR cycle number at which your sample’s reaction curve intersects the threshold line and provides a real signal. Real-time PCR has a reaction curve for each sample and therefore many Cq values.