Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges

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Bio-Scale Mini Bio-Gel P-6 Desalting and Size Exclusion Cartridges

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Size Exclusion Information

Size Exclusion Chromatography

Size exclusion chromatography, also known as gel filtration chromatography, separates molecules by size. The gel matrix consists of spherical beads with pores of a specific size distribution. Separation occurs when molecules of different sizes are excluded from the pores. As small molecules diffuse into the pores, their flow through the column is retarded, while large molecules bypass the pores and are eluted in the column’s void volume. Consequently, molecules are separated according to size and elute in order of decreasing molecular weight.

Operating conditions and selection of media depend on the application and the desired resolution. A common method used in size exclusion chromatography is group separation, which includes desalting and fractionation.
Desalting — The molecule of interest is eluted in the void volume, while smaller molecules are retained in the gel pores; to achieve the desired separation, the matrix should have an exclusion limit smaller than the molecule of interest.
Fractionation — Molecules with a range of molecular weights are separated within the gel matrix; with this method, the molecules of interest should fall within the fractionation range of the gel.

Common applications include protein fractionation and molecular weight determination, nucleic acid separation, plasmid purification, and polysaccharide fractionation.

Resolution depends on particle size, pore size, flow rate, column dimensions, and sample volume. Generally, the highest resolution is obtained with low flow rates (2–10 cm/hr), long, narrow columns, small-particle-size gels, small sample volumes (1–5% of the total bed volume), 2-fold difference in molecular weights, and a sample viscosity that is the same as the eluent. For desalting, the sample volume can be as much as 30–40% of the total bed volume, and shorter, wider columns may be used.

For a list of antibody purification applications, request bulletin 1801. Protein applications are given in bulletin 1802.

Bio-Gel P Polyacrylamide Gels

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylene-bis-acrylamide. Bio-Gel P gels:

  • Are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
  • Are extremely hydrophilic and essentially nonionic
  • Provide efficient, gentle gel filtration of sensitive compounds
  • Do not support microbial growth or leach carbohydrates (due to their synthetic composition), as dextrose and agarose gels can

Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C.

The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides, peptides, and tannins without altering the exclusion properties of the gels. Formamide may also be used at full strength since Bio-Gel P gels are completely swollen in this solvent.

Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilicity ensures little or no interaction between the gel and sample molecules.

For more information, request bulletin 2068.

Properties
 
Hydrated Bead
Size (µm)
Hydrated Bed
Volume Mass Dry Gel
Typical Flow
Rates (cm/hr)*
Molecular Weight
Fractionation Range
Bio-Gel P-2 gel, fine
Bio-Gel P-2 gel, extra fine
45–90
<45
3 ml/g
5–10
<10
100–1,800
Bio-Gel P-4 gel, medium
Bio-Gel P-4 gel, fine
Bio-Gel P-4 gel, extra fine
90–180
45–90
<45
4 ml/g
15–20
10–15
<10
800–4,000
Bio-Gel P-6 gel, medium
Bio-Gel P-6 gel, fine
Bio-Gel P-6 gel, extra fine
Bio-Gel P-6DG gel
90–180
45–90
<45
90–180
6.5 ml/g
15–20
10–15
<10
15–20
1,000–6,000
Bio-Gel P-10 gel, medium
Bio-Gel P-10 gel, fine
90–180
45–90
7.5 ml/g
15–20
10–15
1,500–20,000
Bio-Gel P-30 gel, medium
Bio-Gel P-30 gel, fine
90–180
45–90
9 ml/g
7–13
6–11
2,500–40,000
Bio-Gel P-60 gel, medium
Bio-Gel P-60 gel, fine
90–180
45–90
11 ml/g
4–6
3–5
3,000–60,000
Bio-Gel P-100 gel, medium
Bio-Gel P-100 gel, fine
90–180
45–90
12 ml/g
4–6
3–5
50,000–100,000
* Flow rates determined in a 1.5 x 70 cm column using a hydrostatic pressure head-to-bed ratio of 1:1.

These columns are 5 ml, 10 ml, and 50 ml Bio-Scale Mini Cartridges filled with Bio-Gel P-6 Support. They are used for desalting or buffer exchange using gel filtration (size exclusion) chromatography.
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Ordering Information


  • Description
  • Ordering
  • Accessories
  • Documents

Description

Bio-Scale Mini cartridges filled with Bio-Gel P-6 gel filtration (size exclusion) chromatography support are designed for direct connection to BioLogic™ LP chromatography systems and the Profinia™ protein purification system. Luer fittings enable easy connection to any chromatography system, a peristaltic pump, or a syringe for simple step elution.

Features and Benefits

  • Ideal for desalting or buffer exchange
  • Convenient, ready-to-use, disposable cartridges — 5 ml, 10 ml, or 50 ml
  • Double-walled cartridge design* provides extra durability for runs at pressures up to 45 psi
  • Polypropylene construction compatible with aqueous buffers commonly used for protein separation

More Information

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.







Related Products

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.


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Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 5 ml

7324502
Pkg of 1, 5 ml, prepacked gel filtration column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Bio-Gel P-6 Desalting Column, 1 x 5 ml (12009238)
Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 5 ml

7324504
Pkg of 5, 5 ml, prepacked gel filtration column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Bio-Gel P-6 Desalting Columns, 5 x 5 ml (12009239)
Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 10 ml

7325304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price: 
 
$441.00 
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Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 50 ml

7325312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

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$431.00 
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Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 50 ml

7325314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

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$1,756.00 
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Accessories

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Luer to M6 Adaptor Fittings Kit

7320111
Low-pressure adaptor fittings kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system

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$170.00 
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Luer to 10-32 Adaptor Fittings Kit

7320112
Low-pressure adaptor fittings kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system

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$111.00 
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Luer to BioLogic™ and NGC™ Systems Fittings Kit

7320113
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow™ and NGC Systems

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$68.00 
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Documents

Number Description Options
10006995 Bio-Scale Mini Bio-Gel® P-6 Desalting Cartridges, 10 and 50 ml, Instruction Manual, Rev C Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5742 Profinity eXact Purification Methods on the Profinia System, Rev. A Click to download
5725 Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A Click to download
5712 The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A Click to download
5514 Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A Click to download
6725 From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification, Ver C Click to download
6745 Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis, Ver C Click to download
6770 Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis (Poster), Ver A Click to download

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