Foresight Prepacked Chromatography Columns

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Foresight Prepacked Chromatography Columns

Overview

Foresight™ Filter Plates and Columns Workflow

Foresight chromatography filter plates and columns are prepacked with a range of Bio-Rad’s process chromatography media, offering process scientists convenience and reliability for their high-throughput (HT) experimentation needs. Their robust design allows process scientists to use the prepacked formats through the entire purification development cycle, from HT media screening to small-scale methods development and scale-up optimization.
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Purification scale-up workflow shows the use of HT screening and design of experiments (DOE) to define an operational window for biomanufacturing.

 

Using High-Throughput (HT) Methods to Accelerate DOE

High-throughput experimentation (HTE) was used in this case study in concert with design of experiments (DOE) methodology to study the mass recovery of a basic therapeutic protein (pI 9.2–9.5) using a hydrophobic cation exchange mixed-mode resin.

Foresight columns are prepacked with a range of Bio-Rad’s process chromatography resin. They have a robust design that provides convenience and reliability for process scientists and may be used for resin screening experiments and/or small-scale method development.
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Ordering Information


  • Description
  • Specifications
  • Ordering
  • Fittings
  • Documents

Description

Foresight Prepacked Chromatography Columns have a robust design that provides convenience and reliability for process scientists. Foresight Columns may be used for media screening experiments and/or small-scale method development. Through design of experiments (DOE) utilizing Foresight Columns, an operational window can be defined to aid in developing a quality by design (QbD) process. The design of the column ensures leak-free operation at pressures up to 30 bar.* The columns allow faster, more convenient experimental setups.

Features and Benefits

  • Convenience — prepacked column is ready for equilibration in the buffer of choice. Multiple bed heights are available with the same column diameter that allow straightforward comparative evaluation
  • Reliability — each column is individually packed under optimum compression, ensuring consistent experimental results

The Foresight Columns Instruction Manual describes how to use Foresight Columns prepacked with Bio-Rad's Chromatography Resins.

Other formats available:

Learn more about Foresight Prepacked Column and Plate formats and applications.

Chromatography Resins Available in Foresight Prepacked Columns and Plates

Chromatography Type
Foresight 1 ml Column
Foresight 5 ml Column
Strong Anion Exchange
7324732
UNOsphere Q Column
7324752
UNOsphere Q Column
Strong Anion Exchange
12007020
Nuvia HP-Q Column
12007021
Nuvia HP-Q Column
Strong Anion Exchange
7324721
Nuvia Q Column
7324741
Nuvia Q Column
Strong Cation Exchange
7324730
UNOsphere S Column
7324750
UNOsphere S Column
Strong Cation Exchange
7324723
Nuvia HR-S Column
7324740
Nuvia HR-S Column
Strong Cation Exchange
7324720
Nuvia S Column
7324740
Nuvia S Column
Metal Affinity
12004038
Nuvia IMAC Ni-Charged Column
12004037
Nuvia IMAC Ni-Charged Column
Mixed-Mode: Hydrophobic Anionic
12007392
Nuvia aPrime 4A Column
12007393
Nuvia aPrime 4A Column
Mixed-Mode: Hydrophobic Cationic
7324722
Nuvia cPrime Column
7324742
Nuvia cPrime Column
Mixed-Mode: Cationic, Metal Affinity
7324735
CHT Type I Column, 40 μm
7324755
CHT Type I Column, 40 μm
Mixed-Mode: Cationic, Metal Affinity
7324736
CHT Type II Column, 40 μm
7324756
CHT Type II Column, 40 μm
Mixed-Mode: Cationic, Metal Affinity
12003150
CHT XT Column, 40 μm
12003149
CHT XT Column, 40 μm
Mixed-Mode: Cationic, Metal Affinity
7324737
MPC Type I Column, 40 μm
7324757
MPC Type I Column, 40 μm

* Please refer to each resins' manual for pressure limitations. Do not exceed the resins' pressure limit during operation of the prepacked column.


Specifications

1- and 5-ml Columns  
Connectors 10–32 fittings
Column material Polyethylene (HDPE)
Frit material Polypropylene/polyethylene
Autoclavability Not autoclavable
Shipping Conditions  
Ion exchangers  
  Nuvia™ S, Q and UNOsphere™ S, Q Media 20% ethanol + 150 mM NaCl
CHT™ and MPC™ Media 20% EtOH + 10mM sodium phosphate, pH 6.8
Nuvia cPrime, IMAC, aPrime & UNOsphere SUPrA Media 20% ethanol
1-ml Column  
Inner diameter, mm 8
Length, mm 20
Maximum pressure, bar (psi) Follow guidelines for each resin
Recommended flow rate, ml/min (cm/hr) 0.5–1 (59.5–119)
Maximum flow rate, ml/min (cm/hr) 8 (952)
5-ml Column  
Inner diameter, mm 8
Length, mm 100
Maximum pressure, bar (psi) Follow guidelines for each resin
Recommended flow rate, ml/min (cm/hr) 2.5–5 (297.5–595)
Maximum flow rate, ml/min (cm/hr) 8 (952)

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Foresight™ Nuvia™ Ni-Charged IMAC, 1 ml column

12004038
Pkg of 1, 1 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

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$230.00 
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Foresight™ Nuvia™ Ni-Charged IMAC, 5 ml column

12004037
Pkg of 1, 5 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

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$435.00 
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Foresight™ Nuvia™ HP-Q Column, 1 ml

12007020
1 ml, column prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size

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$255.00 
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Foresight Nuvia HP-Q Column, 5 ml

12007021
Pkg of 1, 5 ml, column prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size

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$485.00 
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Foresight™ CHT™ XT Column

12003150
Pkg of 1, 1 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

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$230.00 
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Foresight™ CHT™ XT Column, 5 ml

12003149
Pkg of 1, 5 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

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$455.00 
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Foresight Nuvia aPrime 4A Column, 1 ml

12007392
Pkg of 1, 1 ml, column prepacked with Nuvia aPrime 4A, 50 µm particle size

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$230.00 
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Foresight Nuvia aPrime 4A Column, 5 ml

12007393
Pkg of 1, 5 ml, column prepacked with Nuvia aPrime 4A, 50 µm particle size

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$440.00 
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Foresight™ MPC™ Type I Column, 40 µm, 1 ml

7324737
Pkg of 1, 1 ml, column prepacked with MPC Type I Mixed-Mode Chromatography Resin, 40 µm particle size

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$225.00 
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Foresight™ MPC™ Type I Column, 40 µm, 5 ml

7324757
Pkg of 1, 5 ml, column prepacked with MPC Type I Mixed-Mode Chromatography Resin, 40 µm particle size

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$450.00 
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Foresight™ Nuvia™ HR-S Column, 1 ml

7324723
Pkg of 1, 1 ml, column prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin

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$205.00 
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Foresight™ Nuvia™ HR-S Column, 5 ml

7324743
Pkg of 1, 5 ml, column prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin

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$360.00 
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Foresight™ UNOsphere SUPrA™ Column, 1 ml

7324729
Pkg of 1, 1 ml, column prepacked with UNOsphere SUPrA Affinity Chromatography Resin

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$215.76 
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Foresight™ UNOsphere SUPrA™ Column, 5 ml

7324749
Pkg of 1, 5 ml, column prepacked with UNOsphere SUPrA Affinity Chromatography Resin

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$570.00 
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Foresight™ UNOsphere™ Q Column

7324732
Pkg of 1, 1 ml, column prepacked with UNOsphere Q Strong Anion Exchange Chromatography Media

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$170.00 
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Foresight™ UNOsphere™ Q Column

7324752
Pkg of 1, 5 ml, column prepacked with UNOsphere Q Strong Anion Exchange Chromatography Media

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$320.00 
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Foresight™ UNOsphere™ S Column

7324730
Pkg of 1, 1 ml, column prepacked with UNOsphere S Strong Cation Exchange Chromatography Media

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$170.00 
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Foresight™ UNOsphere™ S Column

7324750
Pkg of 1, 5 ml, column prepacked with UNOsphere S Strong Cation Exchange Chromatography Media

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$320.00 
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Foresight™ Nuvia™ Q Column

7324721
Pkg of 1, 1 ml, column prepacked with Nuvia Q Strong Anion Exchange Chromatography Media

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$195.00 
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Foresight™ Nuvia™ Q Column

7324741
Pkg of 1, 5 ml, column prepacked with Nuvia Q Strong Anion Exchange Chromatography Media

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$350.00 
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Foresight™ Nuvia™ S Column

7324720
Pkg of 1, 1 ml, column prepacked with Nuvia S Strong Cation Exchange Chromatography Media

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$195.00 
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Foresight™ Nuvia™ S Column

7324740
Pkg of 1, 5 ml, column prepacked with Nuvia S Strong Cation Exchange Chromatography Media

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$350.00 
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Foresight™ Nuvia™ cPrime™ Column

7324722
Pkg of 1, 1 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

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$225.00 
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Foresight™ Nuvia™ cPrime™ Column

7324742
Pkg of 1, 5 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

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$440.00 
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Foresight™ CHT™ Type I Column

7324735
Pkg of 1, 1 ml, column prepacked with CHT Ceramic Hydroxyapatite Type I Multimodal Chromatography Media, 40 μm particle size

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$230.00 
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Foresight™ CHT™ Type I Column

7324755
Pkg of 1, 5 ml, column prepacked with CHT Ceramic Hydroxyapatite Type I Multimodal Chromatography Media, 40 μm particle size

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$450.00 
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Foresight™ CHT™ Type II Column

7324736
Pkg of 1, 1 ml, column prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size

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$225.00 
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Foresight™ CHT™ Type II Column

7324756
Pkg of 1, 5 ml, column prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size

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$450.00 
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Fittings

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

3.2 mm Barb to Male Luer

7318226
Pkg of 25, 3.2 mm barb to male luer, polypropylene

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$52.00 
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1.6 mm Barb to Male Luer

7318225
Pkg of 25, 1.6 mm barb to male luer, polypropylene

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$52.00 
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0.8 mm Barb to Male Luer

7318224
Pkg of 25, 0.8 mm barb to male luer, polypropylene

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$52.00 
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Fitting Adapters ¼"-28F to 10-32M

7500564
Pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic DuoFlow

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$69.00 
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Long Fingertight Fittings

7601311
Fitting for tubing

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$60.00 
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Documents

Number Description Options
10024831 Instruction Manual, Foresight™ Chromatography Media Columns 1 and 5 ml, Rev A Click to download
6297 Foresight Prepacked Plates and Columns Product Information Sheet Click to download
10027565 Instruction Manual, Foresight™ RoboColumn Units 200 and 600 µl, Rev A Click to download
10024413 Instruction Manual, Foresight™ Chromatography Media Filter Plates, Rev A Click to download
D116698 Instruction Manual, Nuvia™ IMAC Resin Click to download
10023853 Instruction Manual, Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin, Rev B Click to download
10033316 Instruction Manual, Nuvia HR-S Strong Cation Exchange Media, Rev A Click to download
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
10014430 Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media, Rev C Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up Click to download [ Add to Cart (Free) ]
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
6982 Mixed-Mode Resins/Media Selection Guide Click to download [ Add to Cart (Free) ]
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6786 Monoclonal Antibody Purification Resin Selection Card, Ver A Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6791 Virus Purification Resin Selection Card, Ver A Click to download
6792 Aggregate Purification Resin Selection Card, Ver A Click to download
6530 Protein Purification Workflow Development Using the Bio-Rad NGC Chromatography System and Stain-Free Gel Technology Application Note Click to download [ Add to Cart (Free) ]
6719 Development of an Efficient Manufacturing Process for Adenovirus, Ver A Click to download

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