The EconoFit IMAC and Desalting Column Pack is designed to facilitate the automated tandem or 2-D chromatography purification of histidine-tagged proteins. Use the Nuvia IMAC Column to bind and elute target protein followed by a Bio-Gel P-6 Desalting Column to quickly and efficiently buffer exchange the eluate to remove imidazole.
EconoFit IMAC and Desalting Column Pack Components
The EconoFit IMAC and Desalting Column Pack consists of the following columns:
|Packed Media (5 ml)||Catalog Number|
|Nuvia IMAC, Ni-Charged||12009286|
Immobilized metal affinity chromatography (IMAC) is an excellent chromatography technique for purification of histidine-tagged proteins. The principle of IMAC is based on the affinity that histidine has for metal ions. Side chains on the nitrilotriacetic acid (NTA) functional ligand selectively bind recombinant histidine-tagged proteins when the resin is charged with Ni2+ or other metals. The advantage of this technique is that proteins can often be purified close to homogeneity in a single step.
EconoFit Nuvia IMAC Columns
EconoFit Nuvia IMAC Columns are packed with Bio-Rad’s specially designed Nuvia IMAC Ni-Charged Resin. Characteristics such as the polymeric nature, optimized ligand density, and open pore structure of the Nuvia IMAC Bead result in superb mechanical strength with high stringency, low nonspecific binding, and the ability to perform separations at high flow rates.
EconoFit Bio-Gel P-6 Desalting Columns
EconoFit Bio-Gel P-6 Desalting Columns are packed with Bio-Gel P-6DG Gel. This gel consists of hydrophilic, spherical, polyacrylamide beads intended for the purification of proteins, nucleic acids, viruses, plasmids, and other macromolecules. Bio-Gel P-6DG Beads are designed to provide high-efficiency desalting or buffer exchange at low backpressure.
Nuvia IMAC and Bio-Gel P-6 Resins are also available in other prepacked and bulk resin formats enabling scale-up from laboratory- to process-scale purifications.
- NGC Medium-Pressure Chromatography Systems
- Nuvia IMAC Resins
- Foresight Prepacked Chromatography Filter Plates & Columns
- Bio-Gel P Polyacrylamide Gels
- Bio-Scale Mini Bio-Gel P-6 Desalting Cartridges
- Econo-Pac 10DG Desalting Prepacked Gravity Flow Columns
- Bio-Spin and Micro Bio-Spin Size Exclusion Spin Columns
Pkg of 1, 1 ml, prepacked Ni-charged IMAC column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system