EconoFit IMAC and Desalting Column Pack #17007882

EconoFit IMAC and Desalting Column Pack


A pack combining 1 prepacked Nuvia IMAC Column and 1 prepacked Bio-Gel P-6 Desalting Column (both 5 ml, 16 mm x 25 mm) to facilitate purification of histidine-tagged proteins using NGC Systems or any chromatography system



The EconoFit IMAC and Desalting Column Pack is designed to facilitate the automated tandem or 2-D chromatography purification of histidine-tagged proteins. Use the Nuvia IMAC Column to bind and elute target protein followed by a Bio-Gel P-6 Desalting Column to quickly and efficiently buffer exchange the eluate to remove imidazole.

EconoFit IMAC and Desalting Column Pack Components

The EconoFit IMAC and Desalting Column Pack consists of the following columns:

Packed Media (5 ml) Catalog Number
Nuvia IMAC, Ni-Charged 12009286
Bio-Gel P-6DG 12009238


Immobilized metal affinity chromatography (IMAC) is an excellent chromatography technique for purification of histidine-tagged proteins. The principle of IMAC is based on the affinity that histidine has for metal ions. Side chains on the nitrilotriacetic acid (NTA) functional ligand selectively bind recombinant histidine-tagged proteins when the resin is charged with Ni2+ or other metals. The advantage of this technique is that proteins can often be purified close to homogeneity in a single step.

EconoFit Nuvia IMAC Columns

EconoFit Nuvia IMAC Columns are packed with Bio-Rad’s specially designed Nuvia IMAC Ni-Charged Resin. Characteristics such as the polymeric nature, optimized ligand density, and open pore structure of the Nuvia IMAC Bead result in superb mechanical strength with high stringency, low nonspecific binding, and the ability to perform separations at high flow rates.

EconoFit Bio-Gel P-6 Desalting Columns

EconoFit Bio-Gel P-6 Desalting Columns are packed with Bio-Gel P-6DG Gel. This gel consists of hydrophilic, spherical, polyacrylamide beads intended for the purification of proteins, nucleic acids, viruses, plasmids, and other macromolecules. Bio-Gel P-6DG Beads are designed to provide high-efficiency desalting or buffer exchange at low backpressure.

Related Products

Nuvia IMAC and Bio-Gel P-6 Resins are also available in other prepacked and bulk resin formats enabling scale-up from laboratory- to process-scale purifications.



Number Description Options
Instruction Manual, EconoFit Nuvia IMAC, Ni-charged Columns
Instruction Manual, EconoFit Bio-Gel P-6 Desalting Columns
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification, Ver C
A Nuvia IMAC Resin–Based Purification Workflow for the Production of Highly Pure Recombinant Proteins
Cas9 Nuclease Preparation Using EconoFit Columns and the NGC Chromatography System Application Note
Purification of Cas9 Nuclease with a Two-Step Chromatographic Process Application Note
Speciality Beads: Bio-Gel™ Resins Flier, Ver A

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