ENrich High-Resolution Ion Exchange Columns

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Overview

Ion exchange columns optimized for separation of biomolecules at fast flow rates. Columns are prepacked with Q resin for anion exchange chromatography or S resin for cation exchange chromatography.
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Description

Description

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ENrich ion exchange chromatography columns are designed for high-resolution separation of proteins and other biomolecules at fast flow rates.

These chromatography columns contain either Q resin for separation by anion exchange or S resin for separation by cation exchange. Both resins have a particle size of 10 µm.

  • The 1 ml ENrich columns can complete a high-resolution separation in about 20 minutes — Q resin (780-0001) and S resin (780-0021).
  • ENrich columns are also available in an 8 ml configuration for larger sample loads and purification scale-up — Q resin (780-0003) and S resin (780-0023).
  • The 1 ml and 8 ml columns operate at a maximum pressure of 500 psi

Features and Benefits of ENrich High-Resolution Ion Exchange Chromatography Columns

  • Provide increased flow rate flexibility with various sample viscosities and temperature conditions
  • Deliver superior quality, reproducible separations
  • High capacity, even at high flow rates
  • Stable at pH 2–12

Applications of ENrich High-Resolution Ion Exchange Chromatography Columns

For purifying biomolecules in a variety of workflows:

  • Final polishing of antibodies
  • Secondary purification after affinity/tag purification
  • High-resolution separation of biomolecules
  • Contaminant removal

The ion exchange columns are compatible with organic acids (1 M acetic acid), 8 M urea, 6 M guanidine HCl, chaotropic agents, detergents, salts, and 1 M NaOH. Alcohol, up to 20% (v/v), can be used to enhance the solubility of nucleotides and peptides.

This support is completely compatible with all commonly used aqueous buffers.

Related Products

ENrich columns are also available for performing size exclusion chromatography.

Bio-Rad offers other ion exchange chromatography columns and ion exchange chromatography resins for packing your own columns.

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.

More Information

ENrich columns are ideal for use on the NGC™ chromatography system or any other medium- to high-pressure chromatography systems.

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution

Ordering

Ordering

items

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enrich-q-5x50-columns-780-0001-thumb.jpg
7800001
Pkg of 1, 5 x 50 mm, 1 ml, prepacked ENrich high-resolution anion exchange column, 130 mg max. protein load, 0.5–2.0 ml/min flow rate, max. operating pressure 500 psi
enrich-q-10x100-columns-780-0003-thumb.jpg
7800003
Pkg of 1, 10 x 100 mm, 8 ml, prepacked high-resolution anion exchange column, 1,000 mg max. protein load, 0.5–2.0 ml/min flow rate, max. operating pressure 500 psi
enrich-s-5x50-columns-780-0021-thumb.jpg
7800021
Pkg of 1, 5 x 50 mm, 1 ml, prepacked high-resolution cation exchange column, 120 mg max. protein load, 0.5–2.0 ml/min flow rate, max. operating pressure 500 psi
enrich-s-10x100-columns-780-0023-thumb.jpg
7800023
Pkg of 1, 10 x 100 mm, 8 ml, prepacked high-resolution cation exchange column, 940 mg max. protein load, 0.5–2.0 ml/min flow rate, max. operating pressure 500 psi

Accessories

Accessories

750-0564_tn.jpg
7500564
Pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic DuoFlow
750-0568_tn.jpg
7500568
Fittings kit, includes 2 nuts and 4 ferrules to connect UNO Column to an HPLC system, for use with BioLogic DuoFlow™ and NGC™ Systems

Documents

Documents

Number Description Options
10027324
Instruction Manual, ENrich™ Q and S High-Resolution Ion Exchange Columns, Rev E
6395
ENrich™ High-Resolution Protein Purification Columns Product Information Sheet, Rev C
[ Add to Cart (Free) ]
6484
High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B
[ Add to Cart (Free) ]
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
6529
Protein Purification and Crystallization of Diabetes Related Peroxisome Proliferator–Activated Receptor-γ (PPARγ), Rev A
6725
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification, Ver C
6745
Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis, Ver C
6749
Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes, Ver A
[ Add to Cart (Free) ]
6770
Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis (Poster), Ver A