Overview
ENrich ion exchange chromatography columns are designed for high-resolution separation of proteins and other biomolecules at fast flow rates.
These chromatography columns contain either Q resin for separation by anion exchange or S resin for separation by cation exchange. Both resins have a particle size of 10 µm.
- The 1 ml ENrich columns can complete a high-resolution separation in about 20 minutes — Q resin (780-0001) and S resin (780-0021).
- ENrich columns are also available in an 8 ml configuration for larger sample loads and purification scale-up — Q resin (780-0003) and S resin (780-0023).
- The 1 ml and 8 ml columns operate at a maximum pressure of 500 psi
Features and Benefits of ENrich High-Resolution Ion Exchange Chromatography Columns
- Provide increased flow rate flexibility with various sample viscosities and temperature conditions
- Deliver superior quality, reproducible separations
- High capacity, even at high flow rates
- Stable at pH 2–12
Applications of ENrich High-Resolution Ion Exchange Chromatography Columns
For purifying biomolecules in a variety of workflows:
- Final polishing of antibodies
- Secondary purification after affinity/tag purification
- High-resolution separation of biomolecules
- Contaminant removal
The ion exchange columns are compatible with organic acids (1 M acetic acid), 8 M urea, 6 M guanidine HCl, chaotropic agents, detergents, salts, and 1 M NaOH. Alcohol, up to 20% (v/v), can be used to enhance the solubility of nucleotides and peptides.
This support is completely compatible with all commonly used aqueous buffers.
Related Products
ENrich columns are also available for performing size exclusion chromatography.
Bio-Rad offers other ion exchange chromatography columns and ion exchange chromatography resins for packing your own columns.
Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:
- Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
- Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
- Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)
Bio-Rad chromatography systems:
- BioLogic LP low-pressure chromatography systems
- BioLogic DuoFlow™ medium-pressure chromatography systems
- NGC™ medium-pressure chromatography systems
- Profinia affinity chromatography protein purification system
- Low-pressure chromatography columns and cartridges
* U.S.patent 7,208,087.
More Information
ENrich columns are ideal for use on the NGC™ chromatography system or any other medium- to high-pressure chromatography systems.
Learn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution
Ordering
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Pkg of 1, 5 x 50 mm, 1 ml, prepacked ENrich high-resolution anion exchange column, 130 mg max. protein load, 1 ml/min recommended flow rate, max. operating pressure 500 psi
Pkg of 1, 10 x 100 mm, 8 ml, prepacked high-resolution anion exchange column, 1,000 mg max. protein load, 4 ml/min recommended flow rate, max. operating pressure 500 psi
Pkg of 1, 5 x 50 mm, 1 ml, prepacked high-resolution cation exchange column, 120 mg max. protein load, 1 ml/min recommended flow rate, max. operating pressure 500 psi
Pkg of 1, 10 x 100 mm, 8 ml, prepacked high-resolution cation exchange column, 940 mg max. protein load, 4 ml/min recommended flow rate, max. operating pressure 500 psi
Accessories
Fittings kit, includes 2 nuts and 4 ferrules to connect UNO Column to an HPLC system, for use with BioLogic DuoFlow and NGC Systems