ENrich High-Resolution Size Exclusion Columns

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ENrich Size Exclusion High-Resolution Columns

Overview

ENrich™ High-Resolution Size Exclusion Columns Instruction Manual

ENrich High-Resolution SEC 70 and SEC 650 Size Exclusion Columns can be used with the NGC™ Chromatography System for rapid and reproducible high-resolution separation of biomolecules. Download the instruction manual for complete details on column characteristics, use, and maintenance.

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Columns for separation of biomolecules by size exclusion chromatography at fast flow rates. Prepacked with 10 µm polymeric resin.
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  • Accessories
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Description

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ENrich size exclusion chromatography columns are designed for high-resolution separation of proteins and other biomolecules at fast flow rates.

These chromatography columns contain polymeric resin to separate biomolecules by their size.

ENrich size exclusion columns are available in two particle sizes:

  • ENrich SEC 70 10 x 300 mm column (780-1070) — for separating biomolecules in the 500 Da to 70 kDa range
  • ENrich SEC 650 10 x 300 mm column (780-1650) — for separating biomolecules in the 5 to 650 kDa range
  • Both ENrich columns operate at a maximum pressure of 600 psi

Features and Benefits of ENrich Size Exclusion Chromatography Columns

  • Flexible flow rate with different sample viscosities and temperature conditions
  • Superior quality, reproducible separations
  • Faster flow rates than similar media
  • Stable from pH 2 to 12

Applications of ENrich Size Exclusion Chromatography Columns

For purifying biomolecules in a variety of workflows:

  • High-resolution separation of biomolecules
  • Contaminant removal
  • Molecular size analysis
  • Purity determination

These size exclusion columns are compatible with organic acids (1 M acetic acid), 8 M urea, 6 M guanidine HCl, chaotropic agents, detergents, salts, and 1 M NaOH. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides and peptides.

This support is completely compatible with all commonly used aqueous buffers.

Related Products

ENrich columns are also available in a prepacked format for performing ion exchange chromatography.

Bio-Rad offers other size exclusion chromatography resins for packing your own columns.

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.

More Information

ENrich columns are ideal for use on the NGC™ chromatography system or any other medium- to high-pressure chromatography systems.

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.


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ENrich™ SEC 70 10 x 300 Column

7801070
Pkg of 1, 10 x 300 mm, 24 ml, prepacked high-resolution SEC 70 size exclusion column, size range 500–70,000 Da, 0.5–1.5 ml/min flow rate, max. operating pressure 600 psi

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$2,196.00 
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ENrich™ SEC 650 10 x 300 Column

7801650
Pkg of 1, 10 x 300 mm, 24 ml, prepacked high-resolution SEC 650 size exclusion column, size range 5k–650k Da, 0.75–2.0 ml/min flow rate, max. operating pressure 600 psi

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$2,196.00 
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Fitting Adapters ¼"-28F to 10-32M

7500564
Pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic DuoFlow

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$69.00 
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Documents

Number Description Options
6395 ENrich™ High-Resolution Protein Purification Columns Product Information Sheet, Rev C Click to download [ Add to Cart (Free) ]
10027325 Instruction Manual, ENrich™ High-Resolution Size Exclusion Columns, Rev A Click to download
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6530 Protein Purification Workflow Development Using the Bio-Rad NGC Chromatography System and Stain-Free Gel Technology Application Note Click to download [ Add to Cart (Free) ]
6529 Protein Purification and Crystallization of Diabetes Related Peroxisome Proliferator–Activated Receptor-γ (PPARγ), Rev A Click to download
6745 Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis, Ver C Click to download
6749 Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes, Ver A Click to download [ Add to Cart (Free) ]
6770 Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis (Poster), Ver A Click to download

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