Bio-Scale Mini Profinity eXact cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with Profinity eXact purification resin. Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The cartridges are used for on-column purification and cleavage of recombinant proteins using the Profinity eXact fusion-tag system. The novel Profinity eXact fusion-tag system generates a highly purified recombinant protein containing only its native amino acid sequence in a single step, and in a fraction of the time of other methods.
Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic™ systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini Profinity eXact cartridges can also be run on the Profinia protein purification system.
* U.S.patent 7,208,087.