Bio-Scale™ Mini DEAE Affi-Gel® Blue Cartridges

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Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732–4120 732–4122 732–4124
UNOsphere™ Q NOsphereQ Strong anion exchange –N+(CH3)3 180 mg BSA 732–4100 732–4102 732–4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732–4110 732–4112 732–4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732–4140 732–4142 732–4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732–4130 732–4132 732–4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732–4632 732–4634
Antibody Purification
Protein A Affi-Prep® protein A Affinity Recombinant protein A 30 mg/ml human IgG
732–4200, 1 x 1 ml 732–4201, 5 x 1 ml 732–4202, 1 x 5 ml
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732–4600 732–4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG
732–4322 732–4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG
732–4332 732–4334
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732–4610 732–4612 732–4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732–4642 732–4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5 ml 5 x 5 ml 5 x 10 ml 1 x 50 ml 5 x 50 ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732–
4502
732–
4504
732–
5304
732–
5312
732–
5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.

** IMAC and GST cartridges are designed for use with the Profinia protein purification system.

*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Bio-Scale Mini DEAE Affi-Gel Blue cartridges are 5 ml cartridges filled with DEAE Affi-Gel Blue support. DEAE Affi-Gel Blue gel is a bifunctional affinity gel containing Cibacron Blue F3GA dye covalently attached to DEAE Bio-Gel A. The dye binds albumin, proteases, and other complement proteins, while the DEAE group binds remaining acidic proteins. DEAE Affi-Gel Blue gel offers an economical alternative to protein A affinity chromatography; its features include:

  • Single-step IgG purification from serum (the eluted IgG contains a small amount of transferrin)
  • Minimal sample preparation (no dialysis or desalting)
  • No detectable proteolytic activity in the eluted IgG fraction

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

*U.S.patent 7,208,087.

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.  
Bio-Scale Mini DEAE Affi-Gel Blue Cartridge

732-4632
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $153.00
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Bio-Scale Mini DEAE Affi-Gel Blue Cartridges

732-4634
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $522.00
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Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev A Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
10006402 Bio-Scale Mini Affi-Gel Blue and DEAE Affi-Gel Blue Cartridges, 5 ml, Instruction Manual, Rev B Click to download