Bio-Scale Mini DEAE Affi-Gel Blue cartridges are 5 ml cartridges filled with DEAE Affi-Gel Blue support. DEAE Affi-Gel Blue gel is a bifunctional affinity gel containing Cibacron Blue F3GA dye covalently attached to DEAE Bio-Gel A. The dye binds albumin, proteases, and other complement proteins, while the DEAE group binds remaining acidic proteins. DEAE Affi-Gel Blue gel offers an economical alternative to protein A affinity chromatography; its features include:
- Single-step IgG purification from serum (the eluted IgG contains a small amount of transferrin)
- Minimal sample preparation (no dialysis or desalting)
- No detectable proteolytic activity in the eluted IgG fraction
Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.