CFX Connect Real-Time PCR Detection System

The CFX Connect Real-Time PCR System provides excellent thermal performance for two-target multiplex PCR and routine detection of single fluorophore experiments.

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Overview

The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.

Key Features and Benefits

The CFX Connect Real-Time PCR Detection System offers:

Easy startup — obtain great results right away with factory-calibrated dyes, quick setup, and intuitive software
Effortless optimization — save time and reduce costs by optimizing assays in a single run using the thermal gradient
Powerful data analysis tools — quickly and accurately validate and analyze data with the advanced analysis modules of CFX Maestro Software
Accelerated publication submission — include MIQE annotations and generate RDML files using Biogazelle's qbase+ Software

Related Technologies

Related Products

Specifications

Chassis

CFX Connect

Maximum ramp rate, °C/sec

Average ramp rate, °C/sec

3.3

Heating and cooling method

Peltier

Lid, °C

Heats up to 105

Temperature

Range, °C

0–100

Accuracy, °C

±0.2 of programmed target at 90°C

Uniformity, °C

±0.4 well-to-well within 10 sec of arrival at 90°C

Gradient

Operational range, °C

30–100

Programmable span, °C

1–24

Optical Detection

Excitation

3 filtered LEDs

Detection

3 filtered photodiodes

Range of excitation/emission wavelengths, nm

450–580

Sensitivity

Detects 1 copy of target sequence in human genomic DNA

Dynamic range

10 orders of magnitude

Scan Time

All channels, sec

FAM/SYBR^® Green only, sec

Software

Operating systems

Windows 7, Windows 8, Windows 10

Multiplex analysis

Up to 2 targets per well

System

Licensed for real-time PCR

Yes

Sample capacity, wells

Sample size, µl

1–50 (10–25 recommended)

Communication interface

USB 2.0

Electrical approvals

IEC, CE

Dimensions (W x L x H), cm/in

33 x 46 x 36/13 x 18 x 14

Weight, kg/lb

21/47

Accessories

(Discontinued) CFX Qualification Plate

1845098

Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water

(Discontinued) PX1 PCR Plate Sealer

1814000

PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

Kits & Reagents

(Discontinued) SsoAdvanced™ Universal SYBR^® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725271

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl₂, SYBR^® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions

(Discontinued) SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725281

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl₂, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions

(Discontinued) iTaq™ Universal SYBR^® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725131

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, enhancers, stabilizers, and ROX Normalization Dyes

(Discontinued) SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml

1725160

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

(Discontinued) SingleShot™ SYBR^® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725085

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR^® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

(Discontinued) iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns

1725038

100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase

(Discontinued) iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl

1708841

100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl₂, stabilizers; 50 reactions of no-RT control supermix

Plastics

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear

HSP9601

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded

HSP9901

Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear

MLL9601

Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples

(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, white

MLL9651

Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, clear

TLS0801

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, white

TLS0851

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

(Discontinued) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear

TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

Downloads

Precision Melt Analysis™ v1.2 Software Upgrade

SOFT-PM-12-PATCH

Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager Software version 2.0 and higher

Download Software

Software Download

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