CFX Connect Real-Time PCR Detection System

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Overview

The CFX Connect Real-Time PCR System provides excellent thermal performance for two-target multiplex PCR and routine detection of single fluorophore experiments.

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Description

Description

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CFX System

Real-Time PCR Tips, Tricks, and Best Practices Guide

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The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.

Key Features and Benefits

The CFX Connect Real-Time PCR Detection System offers:

  • Easy startup — obtain great results right away with factory-calibrated dyes, quick setup, and intuitive software
  • Effortless optimization — save time and reduce costs by optimizing assays in a single run using the thermal gradient
  • Powerful data analysis tools — quickly and accurately validate and analyze data with the advanced analysis modules of CFX Maestro Software
  • Accelerated publication submission — include MIQE annotations and generate RDML files using Biogazelle's qbase+ Software

Specifications

Specifications

Chassis
CFX Connect
Maximum ramp rate, °C/sec
5
Average ramp rate, °C/sec
3.3
Heating and cooling method
Peltier
Lid, °C
Heats up to 105
Temperature
 
Range, °C
0–100
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
 
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
 
Excitation
3 filtered LEDs
Detection
3 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–580
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Scan Time
 
All channels, sec
12
FAM/SYBR® Green only, sec
3
Software
 
Operating systems
Windows 7, Windows 8, Windows 10
Multiplex analysis
Up to 2 targets per well
System
 
Licensed for real-time PCR
Yes
Sample capacity, wells
96
Sample size, µl
1–50 (10–25 recommended)
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x L x H), cm/in
33 x 46 x 36/13 x 18 x 14
Weight, kg/lb
21/47

Ordering

Ordering

items

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1855201
Two-color real-time PCR detection system, includes CFX Connect Thermal Cycler Chassis, CFX Connect Optical Reaction Module, cables

Accessories

Accessories

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1845098
Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water
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(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

Kits & Reagents

Kits & Reagents

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1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
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1725281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
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1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

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1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes
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1725160

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

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1725085
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
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1725038
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase
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1708841
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

Plastics

Plastics

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HSP9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design
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HSP9901

Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

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HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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MLL9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples
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MLL9651
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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TLS0801
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)
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TLS0851
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)
TCS0803
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

Download

Download

Precision Melt Analysis™ v1.2 Software Upgrade
SOFT-PM-12-PATCH
Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager™ Software version 2.0 and higher

Documents

Documents

Number Description Options
6894
Tips, Tricks & Best Practices: The Ultimate qPCR Assay Design Guide
[ Add to Cart (Free) ]
6105
CFX Connect™ Real-Time PCR Detection System Brochure
[ Add to Cart (Free) ]
10000068707
CFX Connect Real-Time PCR Detection System Instrument Guide, Ver A
6900
CFX Maestro Software Brochure
[ Add to Cart (Free) ]
10000126764
CFX Maestro Software User Guide
6102
CFX Connect™ Real-Time PCR Detection System Specifications Sheet, Rev B
6103
CFX Connect™ Real-Time PCR Detection System Flier, Rev A
[ Add to Cart (Free) ]
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
10027341
CFX Qualification Plate — 96-Well Plate Format Product Insert
10022527
Amplification Plastics and Reagents Selection Guide
10021337
Instruction Manual, CFX96 Touch™, CFX96 Touch Deep Well, CFX Connect™, and CFX384 Touch™ Real-Time PCR Detection Systems, Instruction Manual, Rev E
10021652
CFX Connect Real-Time PCR Detection System with CFX Manager Software Installation Quick Guide
12012098
CFX Connect Real-Time PCR Detection System with CFX Maestro Software Installation Quick Guide
10022119
Checking Firmware and Software Updates for the CFX Connect™ Real-Time PCR Detection System, Quick Guide, Rev A
7216
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR
5962
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A
5692
Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A
6047
Optical Design of CFX96™ Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A
5279
Real-Time PCR Applications Guide, Rev B
5990
Gene Expression Analysis Solutions Brochure
[ Add to Cart (Free) ]