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Description
Description
The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.
Key Features and Benefits
The CFX Connect Real-Time PCR Detection System offers:
- Easy startup — obtain great results right away with factory-calibrated dyes, quick setup, and intuitive software
- Effortless optimization — save time and reduce costs by optimizing assays in a single run using the thermal gradient
- Powerful data analysis tools — quickly and accurately validate and analyze data with the advanced analysis modules of CFX Maestro Software
- Accelerated publication submission — include MIQE annotations and generate RDML files using Biogazelle's qbase+ Software
Related Technologies
Related Products
Specifications
Specifications
Chassis
CFX Connect
Maximum ramp rate, °C/sec
5
Average ramp rate, °C/sec
3.3
Heating and cooling method
Peltier
Lid, °C
Heats up to 105
Temperature
Range, °C
0–100
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
Excitation
3 filtered LEDs
Detection
3 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–580
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Scan Time
All channels, sec
12
FAM/SYBR® Green only, sec
3
Software
Operating systems
Windows 7, Windows 8, Windows 10
Multiplex analysis
Up to 2 targets per well
System
Licensed for real-time PCR
Yes
Sample capacity, wells
96
Sample size, µl
1–50 (10–25 recommended)
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x L x H), cm/in
33 x 46 x 36/13 x 18 x 14
Weight, kg/lb
21/47
Ordering
Ordering
items
Use the filters below to refine results!
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(Discontinued) CFX Connect Real-Time PCR Detection System
1855201
Two-color real-time PCR detection system, includes CFX Connect Thermal Cycler Chassis, CFX Connect Optical Reaction Module, cables
Accessories
Accessories
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(Discontinued) CFX Qualification Plate
1845098
Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water
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(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
Kits & Reagents
Kits & Reagents
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1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
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1725281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
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1725121
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes
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1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes
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(Discontinued) SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml
1725160
1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions
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1725085
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
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(Discontinued) iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns
1725038
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase
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1708841
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix
Plastics
Plastics
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HSP9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design
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(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded
HSP9901
Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design
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HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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MLL9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples
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MLL9651
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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(Discontinued) Microseal 'B' PCR Plate Sealing Film, adhesive, optical
MSB1001
Pkg of 100, optically clear seal for PCR plates
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(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, clear
TLS0801
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)
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(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, white
TLS0851
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)
(Discontinued) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear
TCS0803
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear
Download
Download
Precision Melt Analysis™ v1.2 Software Upgrade
SOFT-PM-12-PATCH
Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager Software version 2.0 and higher
Documents
Documents
Login Required Number Description Options
10000068707
CFX Connect Real-Time PCR Detection System Instrument Guide, Ver A
10000126764
CFX Maestro Software User Guide
6102
CFX Connect™ Real-Time PCR Detection System Specifications Sheet, Rev B
10027341
CFX Qualification Plate — 96-Well Plate Format Product Insert
10022527
Amplification Plastics and Reagents Selection Guide
10021337
Instruction Manual, CFX96 Touch™, CFX96 Touch Deep Well, CFX Connect™, and CFX384 Touch™ Real-Time PCR Detection Systems, Instruction Manual, Rev E
10021652
CFX Connect Real-Time PCR Detection System with CFX Manager Software Installation Quick Guide
12012098
CFX Connect Real-Time PCR Detection System with CFX Maestro Software Installation Quick Guide
10022119
Checking Firmware and Software Updates for the CFX Connect™ Real-Time PCR Detection System, Quick Guide, Rev A
7216
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR
5962
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A
5692
Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A
6047
Optical Design of CFX96™ Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A
5279
Real-Time PCR Applications Guide, Rev B
