SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml) #1725281



5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
Safety Data Sheet

Safety Data Sheet



SsoAdvanced Universal Probes Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

SsoAdvanced universal probes supermix lets you:

  • Carry out high-performance singleplex and duplex reactions — the Sso7d fusion polymerase and advanced formulation enable robust performance in singleplex or duplex real-time PCR reactions, providing the highest data precision and allowing cost and time savings when combining 2 assays in a single well
  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system
  • Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
  • Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation

Instrument Compatibility

SsoAdvanced Universal Probes Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
  • Mx3000P, 3005P, 4000
  • Mastercycler ep realplex 2, 4
  • Corbett Rotor-Gene 3000, 6000, Q
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal Probes Supermix

  • qPCR/real-time PCR
  • Gene expression analysis
  • Absolute quantification
  • Genotyping (allelic discrimination)
  • Mutation detection
  • Pathogen detection
  • Viral detection (load)
  • Characterization of genetically modified organisms (GMO)
  • Genetic profiling

Packaging Options

Number of 20 μl Reactions Total Volume Number of Vials Catalog #
200 2 ml 2 x 1 ml 1725280
500 5 ml 5 x 1 ml 1725281
1,000 10 ml 10 x 1 ml 1725282
2,500 25 ml 5 x 5 ml 1725284
5,000 50 ml 10 x 5 ml 1725285

Related Products

We have developed SsoAdvanced Universal SYBR® Green Supermix, which is available in several formats (200–5,000 reactions).

Bio-Rad offers a complete workflow solution for real-time PCR, including:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patent 7,560,260



Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (minimum usage time) at –20°C
12 months