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Overview
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix
Safety Data Sheet
Description
Description
Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.
Key Features and Benefits
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1 pg total input RNA
- Amplify cDNA for genes with low expression levels
- Fast 26-minute 1-tube cDNA synthesis protocol
- 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination
Packaging Options
| Kit Contents | Supermix Volume, µl | Catalog Number |
| 25 x 20 µl reactions | 100 (1 x 100 µl) | 1708840 |
| 100 x 20 µl reactions | 400 (4 x 100 µl) | 1708841 |
| 500 x 20 µl reactions | 2000 (20 x 100 µl) | 1708841BUN |
See the iScript Reverse Transcription Supermix product page for more information and documentation.
Related Products
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Supporting Documents
- iScript Reverse Transcription Supermix Product Insert
- Amplification Plastics and Reagents Selection Guide