SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml #1725160

SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml

Overview

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

Available Safety Data Sheets
Safety Data Sheet

Safety Data Sheet

Find Certificate of Analysis

Description

Description

Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.

  • Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
  • Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
  • For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays, self-designed assays, and TaqMan Assays

Product Comparison

Product ΔΔCq* Chemistry Reactions Price**
SsoAdvanced PreAmp Supermix 90% within ± 0.75 SYBR® Green and probes 50 x 50 µl Least expensive
TaqMan PreAmp Master Mix 90% within ± 1.5 Probes only 40 x 50 µl Most expensive
PerfeCTa PreAmp 5x Supermix 90% within ± 1.5 SYBR® Green and probes 40 x 50 µl More expensive

 

* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.

 

Simple Workflow

Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.

Simple Workflow for SsoAdvanced PreAmp Supermix

Recommended RT-qPCR Reagents and Assays

Reverse Transcription

qPCR Supermixes

PreAmp and qPCR Assays

More Information

Find out more about the features of the SsoAdvanced™ PreAmp Supermix.

Documents

Documents

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Recommended SKUs

Recommended SKUs