SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml #1725160

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1725160 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [34.200.252.156, 10.232.18.29] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml] [x-amzn-trace-id] = [Root=1-5fc50512-4693227a5fb0610a48ef604b] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1725160] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/sku/1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml LSR en US en-us /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-09-18 04:55:37 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/37 05:00 PM 1579275613146 2020-09-18 04:55:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/37 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"$727.00","name":"SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml","variant":"BRC","id":"1725160","category":"BRC","brand":"BRC"}}},"event":"detail"} 1725160 11/22/13 09:02 AM en_US true internet/product_SKU 2019-05-04 16:19:24 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml /default/main/bio-rad/WORKAREA/default jcihuc1z 2017-11-14 07:07:36 true jcihuc1z templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml LSR N /default/main/bio-rad en 11/22/23 09:07 AM 1555974592289 2019-05-04 16:19:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml 1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml 1 1725160 SsoAdvanced PreAmp Supermix SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml 1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 &micro;l reactions 1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-preamp-supermix-172-5160-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-preamp-supermix-172-5160.jpg SsoAdvanced PreAmp Supermix preview ,VN Karen Moss SsoAdvanced PreAmp Supermix 1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 &micro;l reactions 1725160, ssoadvanced, preamp, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 11/22/13 09:02 AM 11/22/23 09:07 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SsoAdvanced PreAmp Supermix</strong> to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.</p> <ul> <li>Start with as little as 100 pg of cDNA &mdash;&nbsp;amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)</li> <li>Compatible with PrimePCR&trade; PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays</li> <li>For gene expression and genotyping applications &mdash; validated with <a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; PreAmp Assays</a>, self-designed assays, and TaqMan Assays</li> </ul> <h2 class="descHeader">Product Comparison</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Product</strong></td> <td><strong>&Delta;&Delta;Cq*</strong></td> <td><strong>Chemistry</strong></td> <td><strong>Reactions</strong></td> <td><strong>Price**</strong></td> </tr> <tr class="pd_colorbackground"> <td>SsoAdvanced PreAmp Supermix</td> <td>90% within &plusmn; 0.75</td> <td>SYBR<sup>&reg;</sup> Green and probes</td> <td>50 x 50 &micro;l</td> <td>Least expensive</td> </tr> <tr> <td>TaqMan PreAmp Master Mix</td> <td>90% within &plusmn; 1.5</td> <td>Probes only</td> <td>40 x 50 &micro;l</td> <td>Most expensive</td> </tr> <tr class="pd_colorbackground"> <td>PerfeCTa PreAmp 5x Supermix</td> <td>90% within &plusmn; 1.5</td> <td>SYBR<sup>&reg;</sup> Green and probes</td> <td>40 x 50 &micro;l</td> <td>More expensive</td> </tr> </tbody> </table> <br /> <p class="caption" style="margin-left: 13px;">* Relative quantification between nonamplified and preamplified cDNA.<br /> ** As of May 2014.</p> <h2 class="descHeader">Simple Workflow</h2> <p>Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/simple-workflow-ssoadvanced-preamp-supermix-5-12.jpg" alt="Simple Workflow for SsoAdvanced PreAmp Supermix" width="597" height="278" /></p> <h2 class="descHeader">Recommended RT-qPCR Reagents and Assays</h2> <p>Reverse Transcription</p> <ul> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript&trade; Reverse Transcription Supermix for RT-qPCR</a></li> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR</a></li> </ul> <p>qPCR Supermixes</p> <ul> <li><a href="/en-us/product/ssoadvanced-universal-sybr-green-supermix">SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</a></li> <li><a href="/en-us/product/ssoadvanced-universal-probes-supermix">SsoAdvanced&trade; Universal Probes Supermix</a></li> </ul> <p>PreAmp and qPCR Assays</p> <ul> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; PreAmp Assays</a></li> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; Assays</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Find out more about the features of the <a href="/en-us/product/ssoadvanced-preamp-supermix">SsoAdvanced&trade; PreAmp Supermix</a>.</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ssoadvanced-preamp-supermix-172-5160-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ssoadvanced-preamp-supermix-172-5160-desc.html 1 0 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml 1708841 /templatedata/internet/product_SKU/data/ja_JP/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml false $727.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&CartQuote=QT&quantity= 1450 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&AddToDefaultWishList=true&quantity= 41106300 727.00 SKU detail page

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

1725160 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [34.200.252.156, 10.232.18.29] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml] [x-amzn-trace-id] = [Root=1-5fc50512-4693227a5fb0610a48ef604b] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1725160] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/sku/1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml LSR en US en-us /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-09-18 04:55:37 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/37 05:00 PM 1579275613146 2020-09-18 04:55:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/37 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"$727.00","name":"SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml","variant":"BRC","id":"1725160","category":"BRC","brand":"BRC"}}},"event":"detail"} 1725160 11/22/13 09:02 AM en_US true internet/product_SKU 2019-05-04 16:19:24 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml /default/main/bio-rad/WORKAREA/default jcihuc1z 2017-11-14 07:07:36 true jcihuc1z templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml LSR N /default/main/bio-rad en 11/22/23 09:07 AM 1555974592289 2019-05-04 16:19:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml 1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml 1 1725160 SsoAdvanced PreAmp Supermix SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml 1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 &micro;l reactions 1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-preamp-supermix-172-5160-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-preamp-supermix-172-5160.jpg SsoAdvanced PreAmp Supermix preview ,VN Karen Moss SsoAdvanced PreAmp Supermix 1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 &micro;l reactions 1725160, ssoadvanced, preamp, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 11/22/13 09:02 AM 11/22/23 09:07 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SsoAdvanced PreAmp Supermix</strong> to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.</p> <ul> <li>Start with as little as 100 pg of cDNA &mdash;&nbsp;amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)</li> <li>Compatible with PrimePCR&trade; PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays</li> <li>For gene expression and genotyping applications &mdash; validated with <a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; PreAmp Assays</a>, self-designed assays, and TaqMan Assays</li> </ul> <h2 class="descHeader">Product Comparison</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Product</strong></td> <td><strong>&Delta;&Delta;Cq*</strong></td> <td><strong>Chemistry</strong></td> <td><strong>Reactions</strong></td> <td><strong>Price**</strong></td> </tr> <tr class="pd_colorbackground"> <td>SsoAdvanced PreAmp Supermix</td> <td>90% within &plusmn; 0.75</td> <td>SYBR<sup>&reg;</sup> Green and probes</td> <td>50 x 50 &micro;l</td> <td>Least expensive</td> </tr> <tr> <td>TaqMan PreAmp Master Mix</td> <td>90% within &plusmn; 1.5</td> <td>Probes only</td> <td>40 x 50 &micro;l</td> <td>Most expensive</td> </tr> <tr class="pd_colorbackground"> <td>PerfeCTa PreAmp 5x Supermix</td> <td>90% within &plusmn; 1.5</td> <td>SYBR<sup>&reg;</sup> Green and probes</td> <td>40 x 50 &micro;l</td> <td>More expensive</td> </tr> </tbody> </table> <br /> <p class="caption" style="margin-left: 13px;">* Relative quantification between nonamplified and preamplified cDNA.<br /> ** As of May 2014.</p> <h2 class="descHeader">Simple Workflow</h2> <p>Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/simple-workflow-ssoadvanced-preamp-supermix-5-12.jpg" alt="Simple Workflow for SsoAdvanced PreAmp Supermix" width="597" height="278" /></p> <h2 class="descHeader">Recommended RT-qPCR Reagents and Assays</h2> <p>Reverse Transcription</p> <ul> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript&trade; Reverse Transcription Supermix for RT-qPCR</a></li> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR</a></li> </ul> <p>qPCR Supermixes</p> <ul> <li><a href="/en-us/product/ssoadvanced-universal-sybr-green-supermix">SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</a></li> <li><a href="/en-us/product/ssoadvanced-universal-probes-supermix">SsoAdvanced&trade; Universal Probes Supermix</a></li> </ul> <p>PreAmp and qPCR Assays</p> <ul> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; PreAmp Assays</a></li> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; Assays</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Find out more about the features of the <a href="/en-us/product/ssoadvanced-preamp-supermix">SsoAdvanced&trade; PreAmp Supermix</a>.</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ssoadvanced-preamp-supermix-172-5160-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ssoadvanced-preamp-supermix-172-5160-desc.html 1 0 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml 1708841 /templatedata/internet/product_SKU/data/ja_JP/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml false $727.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&CartQuote=QT&quantity= 1450 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&AddToDefaultWishList=true&quantity= 41106300 727.00 SKU detail page
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Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.

  • Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
  • Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
  • For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays, self-designed assays, and TaqMan Assays

Product Comparison

Product ΔΔCq* Chemistry Reactions Price**
SsoAdvanced PreAmp Supermix 90% within ± 0.75 SYBR® Green and probes 50 x 50 µl Least expensive
TaqMan PreAmp Master Mix 90% within ± 1.5 Probes only 40 x 50 µl Most expensive
PerfeCTa PreAmp 5x Supermix 90% within ± 1.5 SYBR® Green and probes 40 x 50 µl More expensive

* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.

Simple Workflow

Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.

Simple Workflow for SsoAdvanced PreAmp Supermix

Recommended RT-qPCR Reagents and Assays

Reverse Transcription

qPCR Supermixes

PreAmp and qPCR Assays

More Information

Find out more about the features of the SsoAdvanced™ PreAmp Supermix.

Number Description Options

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