Overview
1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions
Safety Data Sheet
Description
Description
Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.
- Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
- Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
- For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays, self-designed assays, and TaqMan Assays
Product Comparison
| Product | ΔΔCq* | Chemistry | Reactions | Price** |
| SsoAdvanced PreAmp Supermix | 90% within ± 0.75 | SYBR® Green and probes | 50 x 50 µl | Least expensive |
| TaqMan PreAmp Master Mix | 90% within ± 1.5 | Probes only | 40 x 50 µl | Most expensive |
| PerfeCTa PreAmp 5x Supermix | 90% within ± 1.5 | SYBR® Green and probes | 40 x 50 µl | More expensive |
* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.
Simple Workflow
Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.
Recommended RT-qPCR Reagents and Assays
Reverse Transcription
- iScript™ Reverse Transcription Supermix for RT-qPCR
- iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
qPCR Supermixes
PreAmp and qPCR Assays
More Information
Find out more about the features of the SsoAdvanced™ PreAmp Supermix.
Documents
Documents
Recommended SKUs
Recommended SKUs
