SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml #1725160

1725160 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [34.200.252.156, 10.232.18.29] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml] [x-amzn-trace-id] = [Root=1-5fc50512-4693227a5fb0610a48ef604b] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1725160] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/sku/1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml LSR en US en-us /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-09-18 04:55:37 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/37 05:00 PM 1579275613146 2020-09-18 04:55:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K–12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/37 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 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/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-preamp-supermix-172-5160.jpg SsoAdvanced PreAmp Supermix preview ,VN Karen Moss SsoAdvanced PreAmp Supermix 1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 µl reactions 1725160, ssoadvanced, preamp, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 11/22/13 09:02 AM 11/22/23 09:07 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SsoAdvanced PreAmp Supermix</strong> to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.</p> <ul> <li>Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)</li> <li>Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays</li> <li>For gene expression and genotyping applications — validated with <a href="/en-us/product/pcr-primers-probes-panels">PrimePCR™ PreAmp Assays</a>, self-designed assays, and TaqMan Assays</li> </ul> <h2 class="descHeader">Product Comparison</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Product</strong></td> <td><strong>ΔΔCq*</strong></td> <td><strong>Chemistry</strong></td> <td><strong>Reactions</strong></td> <td><strong>Price**</strong></td> </tr> <tr class="pd_colorbackground"> <td>SsoAdvanced PreAmp Supermix</td> <td>90% within ± 0.75</td> <td>SYBR<sup>®</sup> Green and probes</td> <td>50 x 50 µl</td> <td>Least expensive</td> </tr> <tr> <td>TaqMan PreAmp Master Mix</td> <td>90% within ± 1.5</td> <td>Probes only</td> <td>40 x 50 µl</td> <td>Most expensive</td> </tr> <tr class="pd_colorbackground"> <td>PerfeCTa PreAmp 5x Supermix</td> <td>90% within ± 1.5</td> <td>SYBR<sup>®</sup> Green and probes</td> <td>40 x 50 µl</td> <td>More expensive</td> </tr> </tbody> </table> <br /> <p class="caption" style="margin-left: 13px;">* Relative quantification between nonamplified and preamplified cDNA.<br /> ** As of May 2014.</p> <h2 class="descHeader">Simple Workflow</h2> <p>Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/simple-workflow-ssoadvanced-preamp-supermix-5-12.jpg" alt="Simple Workflow for SsoAdvanced PreAmp Supermix" width="597" height="278" /></p> <h2 class="descHeader">Recommended RT-qPCR Reagents and Assays</h2> <p>Reverse Transcription</p> <ul> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript™ Reverse Transcription Supermix for RT-qPCR</a></li> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript™ Advanced cDNA Synthesis Kit for RT-qPCR</a></li> </ul> <p>qPCR Supermixes</p> <ul> <li><a href="/en-us/product/ssoadvanced-universal-sybr-green-supermix">SsoAdvanced™ Universal SYBR<sup>®</sup> Green Supermix</a></li> <li><a href="/en-us/product/ssoadvanced-universal-probes-supermix">SsoAdvanced™ Universal Probes Supermix</a></li> </ul> <p>PreAmp and qPCR Assays</p> <ul> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR™ PreAmp Assays</a></li> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR™ Assays</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Find out more about the features of the <a href="/en-us/product/ssoadvanced-preamp-supermix">SsoAdvanced™ PreAmp Supermix</a>.</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ssoadvanced-preamp-supermix-172-5160-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ssoadvanced-preamp-supermix-172-5160-desc.html 1 0 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml 1708841 /templatedata/internet/product_SKU/data/ja_JP/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml false $727.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&CartQuote=QT&quantity= 1450 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&AddToDefaultWishList=true&quantity= 41106300 727.00 SKU detail page

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

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Description

Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.

Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays, self-designed assays, and TaqMan Assays

Product Comparison

Product	ΔΔCq*	Chemistry	Reactions	Price**
SsoAdvanced PreAmp Supermix	90% within ± 0.75	SYBR^® Green and probes	50 x 50 µl	Least expensive
TaqMan PreAmp Master Mix	90% within ± 1.5	Probes only	40 x 50 µl	Most expensive
PerfeCTa PreAmp 5x Supermix	90% within ± 1.5	SYBR^® Green and probes	40 x 50 µl	More expensive

* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.

Simple Workflow

Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.

Simple Workflow for SsoAdvanced PreAmp Supermix

Recommended RT-qPCR Reagents and Assays

Reverse Transcription

qPCR Supermixes

PreAmp and qPCR Assays

More Information

Find out more about the features of the SsoAdvanced™ PreAmp Supermix.

Number	Description	Options

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