The Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as animal and plant tissues. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a spin-column format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications. The Aurum total RNA mini kit can also be used for RNA cleanup and desalting.
- High-quality RNA isolation from a wide variety of samples in less than 60 minutes
- RNase-free reagents and plasticware, including DNase I for removal of genomic DNA, at no additional cost
- Rapid silica binding-based purification technology in a spin-column format
- Easy-to-use spin- or vacuum-compatible protocol
Genomic DNA, RNA, and Plasmid Purification Kits
Bio-Rad offers a complete line of products for the isolation and purification of nucleic acids. From the purification of milligram quantities of plasmid DNA for restriction analysis or transformation to the rapid and efficient purification of PCR products, Bio-Rad provides you with high-quality kits that eliminate time-consuming, labor-intensive steps.
when isolating total RNA from small amounts
of starting material (<500,000 cells
or 10 mg of tissue).
developmental stage, tissue type or cell line,
and growth conditions used.
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