Aurum™ Total RNA Mini Kit

Image
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Overview

The Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as animal and plant tissues.
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Description

Description

The Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as animal and plant tissues. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a spin-column format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications. The Aurum total RNA mini kit can also be used for RNA cleanup and desalting.

Features include:

  • High-quality RNA isolation from a wide variety of samples in less than 60 minutes
  • RNase-free reagents and plasticware, including DNase I for removal of genomic DNA, at no additional cost
  • Rapid silica binding-based purification technology in a spin-column format
  • Easy-to-use spin- or vacuum-compatible protocol

Genomic DNA, RNA, and Plasmid Purification Kits
Bio-Rad offers a complete line of products for the isolation and purification of nucleic acids. From the purification of milligram quantities of plasmid DNA for restriction analysis or transformation to the rapid and efficient purification of PCR products, Bio-Rad provides you with high-quality kits that eliminate time-consuming, labor-intensive steps.

Specifications

Specifications

Format
Mini column
Method
Spin- or vacuum-mediated silica binding
Number of preps
50
Maximum starting material amounts
20 mg hard animal tissue
 
40 mg plant or soft to moderately hard animal tissues
 
2 x 106 cultured cells
 
2.4 x 109 bacterial cells
 
3 x 107 yeast cells
Binding capacity
>100 µg purified total RNA
Purity
A260/A280 of 1.9–2.1
RNA size distribution
>200 nucleotides
Preparation time
30–60 min (including DNase I digest)
Maximum elution volume
80 µl (2 elutions of 40 µl each)
Minimum elution volume
30 µl*
Yield**
20 µg/40 mg brain
 
132 µg/40 mg spleen
 
36 µg/40 mg lung
 
32 µg/40 mg potato
 
54 µg/2.4 x 109 E. coli cells
 
28 µg/3 x 107 S. cerevisiae cells
 
28 µg/2 x 106 NIH 3T3cells
 
38 µg/2 x 109 HeLa cells
*The elution volume is reduced to 30µl
when isolating total RNA from small amounts
of starting material (<500,000 cells
or 10 mg of tissue).
 
**Yields will vary depending on the
developmental stage, tissue type or cell line,
and growth conditions used.
 

Ordering

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7326820
DNA-free RNA isolation kit, includes RNA binding columns, capless wash and capped microcentrifuge tubes, lyophilized DNase I, RNase-free reagents, for 50 preps

Accessories

Accessories

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(Discontinued) DNase I
7326828
Pkg of 1 vial, refill reagent for Aurum total RNA kits, RNase-free, lyophilized
732-6826_tn.jpg
7326826
Pkg of 50, spin columns for Aurum total RNA kits
732-6805_tn.jpg
7326805
20 ml, refill reagent for Aurum total RNA kits
732-6804_tn.jpg
7326804
60 ml, refill reagent for Aurum total RNA kits
732-6803_tn.jpg
7326803
150 ml, refill reagent for Aurum total RNA kits, RNase-free
732-6802_tn.jpg
7326802
85 ml, refill reagent for Aurum total RNA kits, RNase-free
732-6801_tn.jpg
7326801
20 ml, refill reagent for Aurum total RNA kits, RNase-free

Documents

Documents

Number Description Options
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
2920
Aurum Total RNA Mini Kit Product Information Sheet, Rev B
2954
Aurum Total RNA Mini Kit Protocol Overview — Vacuum Format
2926
Aurum Total RNA Mini Kit Protocol Overview — Spin Format
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
4110133
Instruction Manual, Aurum Total RNA Mini Kit, Rev B
7331
Protocol for Viral RNA Extraction using Aurum Total RNA Kits, Ver A
6816
Protocol for Multiplex RT-qPCR Analysis Using PrimePCR™ Assays, Ver A