Real-Time PCR Systems
CFX Real-Time PCR Detection Systems are powerful and flexible instruments, featuring 2–5 color multiplexing, advanced optical technology, and precise temperature control with thermal gradients. Our qPCR systems deliver sensitive, reliable detection of both singleplex and multiplex real-time PCR reactions. Our CFX Maestro qPCR Analysis Software, provides superior data collection, statistical analysis, and data visualization capabilities.
qPCR Systems
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Real-Time PCR System |
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Real-Time PCR System |
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Real-Time PCR System |
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| Features |
Powerful 5-channel multiplexing with our best thermal uniformity and modernized data management options. |
Our high-throughput system with 4-channel multiplexing, automation capabilities, and more. |
Accessible qPCR for essential molecular testing. |
Industry-leading technology for large-volume reactions in real-time PCR applications. |
| Wells | 96 | 384 | 96 | 96 |
| Multiplex channels | 5 plus FRET | 4 plus FRET | 2 plus FRET | 5 plus FRET |
| Touch screen | Color | Color | Computer-controlled operation only |
Color |
| Reaction volume | 1–50 µl | 1–30 µl | 1–50 µl | 10–125 µl |
| RUO | CFX Opus 96 Real-Time PCR System | CFX Opus 384 Real-Time PCR System | CFX Duet Real-Time PCR System | CFX Opus Deepwell Real-Time PCR System |
| IVD | CFX Opus 96 Dx Real-Time PCR System | CFX Opus 384 Dx Real-Time PCR System Not Available | Not Available | CFX Opus Deepwell Dx Real-Time PCR System |
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CFX Opus Deepwell Real-Time PCR System
Introducing the CFX Opus Real-Time PCR Systems
Choose the CFX Opus Real-Time PCR System for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab.
Introducing the CFX Opus Real-Time PCR System
Choose the CFX Opus Real-Time PCR System for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab.
CFX Opus Dx Real-Time PCR Detection Systems for In Vitro Diagnostics (IVD)
The CFX Opus Dx Real-Time PCR Detection Systems for In Vitro Diagnostics (IVD) is specifically designed for in vitro diagnostic use in a clinical laboratory.
Real-Time PCR Analysis Software
CFX Maestro Software is built to work with the CFX series of real-time PCR systems and is an easy-to-learn and easy-to-use comprehensive tool for plate setup, data collection, data analysis, and data visualization of real-time PCR results.
CFX Maestro Software Features
- Statistical data — t-tests and one-way ANOVA
- Publication-quality graphing — edit, annotate, and export charts at high resolution
- Reference gene selection tool — stability level of genes is displayed
- PrimePCR Assay integration — plate run files can populate plate setup
Reagents & Plastics Selectors
PCR & qPCR Reagents Selector
Find the best reagents for your PCR, qPCR, and RT-qPCR experiments using our interactive selection tool.
PCR Plastics Selector
Use the PCR Plastics Selector to find the best plates, tubes, seals, and accessories for your PCR and qPCR experiments and determine instrument compatibility.
Resources
Understanding Viral Replication Using Cutting-Edge qPCR & Gene Editing Technologies
Dr. Arturo Diaz describes his research on understanding single-stranded RNA viral replication and optimizing the CRISPR/Cas9 system to potentially identify targets for future broad-spectrum antiviral therapies.
Using CFX Maestro Software to Advance qPCR Research
Dr. Sascha Drewlo uses CFX Maestro Software in his research to investigate the development and role of human placenta in pregnancies by finding intervention strategies and early methods of disease detection.
Amplification Reagents & Plastics
(PDF 8.48 MB)Download this brochure for an overview of all PCR reagents and plastics, their specifications, and performance data.
Excitation & Detection Wavelength Charts
View excitation and detection wavelength ranges for various reporter dyes on the CFX96 Touch and CFX96 Touch Deep Well Systems.
Optical Design
Learn about the optical technology providing precise target quantification in CFX Systems.
Tips, Tricks & Best Practices for qPCR
(PDF 3.28 MB)The Ultimate qPCR Guide.
Applications & Technologies
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR
A snapshot of the critical steps needed to achieve excellent qPCR results.
What is Real-Time PCR (qPCR)?
Learn about the proper design, optimization, analysis, and troubleshooting of real-time polymerase chain reactions.
Digital PCR & Real-Time PCR (qPCR) Choices for Different Applications
Compare the capabilities, advantages, and main applications of digital PCR and qPCR technologies.
Real-Time PCR Troubleshooting
Find possible causes and treatments for common qPCR problems.
Introduction to qPCR Systems
Learn about the different Bio-Rad real-time PCR systems and software available for data analysis.
MIQE & RDML Guidelines
Become familiar with the minimum information guidelines required to publish qPCR experiments and with RDML.
