
Overview
![]() C1000 Touch™ Thermal Cycler |
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Input power | 850 W maximum |
Frequency | 50–60 Hz, single phase |
Display | 8.5 in. LCD display and touch screen |
Ports | 5 USB A, 1 USB B |
Fuses | Two 6.3 A, 250 V, 5 x 20 mm |
Memory | >1,000 typical programs onboard; unlimited with USB flash drive expansion |
Dimensions (W x D x H) | 33 x 46 x 20 cm (13 x 18 x 8") |
Weight | 10 kg (23 lb) |
Temperature control modes | Calculated and block |
PCR license | Yes |
Programming options | Step-based graphical and automatic |
Security features | Optional log-in required mode for regulated environments |
Reporting | Exportable run logs, system error logs |
Onboard software | Windows CE 6.0 |
PC compatibility | Windows XP or higher |
USB peripheral compatibility | Mouse, USB flash drive, bar code reader |
Real-time PCR upgrade | 6-channel CFX96™ optical reaction module 5-channel CFX384™ optical reaction module |
Instant incubation | Yes |
Reaction Module Specifications
![]() 96-Well Fast |
![]() 96–Deep Well |
![]() Dual 48/48-Well Fast |
![]() 384-Well |
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Sample capacity | 96 x 0.2 ml tubes or 1 x 96-well plate |
96 x 0.2 ml tubes, 48 x 0.5 ml tubes, or 1 x 96-well plate |
2 x 48 x 0.2 ml tubes or 2 x 48-well plates |
1 x 384-well plate |
Maximum ramp rate | 5°C/sec | 2.5°C/sec | 4°C/sec | 2.5°C/sec |
Average ramp rate | 3.3°C/sec | 2°C/sec | 3°C/sec | 2°C/sec |
Temperature range | 0–100°C | 0–100°C | 0–100°C | 0–100°C |
Temperature accuracy | ±0.2°C of programmed target at 90°C | ±0.2°C of programmed target at 90°C | ±0.2°C of programmed target at 90°C | ±0.2°C of programmed target at 90°C |
Temperature uniformity | ±0.4°C well-to-well within 10 sec of arrival at 90°C | ±0.4°C well-to-well within 10 sec of arrival at 90°C | ±0.4°C well-to-well within 10 sec of arrival at 90°C | ±0.4°C well-to-well within 10 sec of arrival at 90°C |
Gradient | ||||
Gradient capability | Yes | Yes | Yes | Yes |
Gradient range | 30–100°C | |||
Temperature differential range | 1–24°C |
If you're like most researchers, you want to get results faster. Many factors contribute to the overall time it takes to get PCR results: the time to design, program, and optimize a protocol, the time to run the protocol, and the time to analyze the results. Bio-Rad engineers have developed a complete thermal cycling system that will speed every step of the PCR process to give you what's really important — a shorter time from setup to results.
Protocol autowriter — generates an optimal protocol based on your polymerase, primers, and product length.
Thermal gradient feature — identifies optimal annealing temperature in a single run.
Reduced-mass sample block — fast ramping and settling produce the shortest time to target temperature available in a thermal cycler.
Intuitive software — makes programming faster and easier for PCR and real-time PCR.

The patented* reduced-mass sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced.
Superior Uniformity
Precision of the temperature steps is critical for the rate and efficiency of PCR. To obtain reliable, consistent results, all sample wells must maintain proper temperature throughout each incubation step. The C1000 Touch™ thermal cycler uses six independently controlled thermal electric modules (TEs), the heating and cooling elements of the thermal cycler, to maintain tight temperature uniformity at all points during a run — even while ramping.
Rapid Arrival at Target Temperature
A key component of overall protocol run time is the time required to reach target temperature, which is determined by the average ramp rate and the time needed for the sample block to reach thermal uniformity. Maximum ramp rate is less important because it can fluctuate significantly during the ramp. The C1000 Touch thermal cycler's temperature control produces high average ramp rates and tight uniformity during ramping to yield fast time to target temperature and faster protocol run times. Run times can be dramatically shortened — to less than 30 min — while still producing accurate quantitative results. Now you can tailor your runs around your schedule instead of tailoring your schedule around your runs.

Superior uniformity with rapid arrival at target temperature. 1000-series thermal cyclers exhibit high average ramp rates, rapid settling time, and tight thermal uniformity throughout the ramp. This graph shows the temperature measured by probes in 15 wells across a sample block. The traces are nearly indistinguishable due to the tight uniformity. Note the consistent high average ramp rate throughout heating and cooling.
* U.S. patent 7,632,464.

Thermal Gradient for Easy Protocol Optimization
The programmable temperature gradient allows you to identify the optimal annealing temperature in a single PCR experiment by letting you test eight incubation temperatures at once.
The 1000-series gradient feature uses dynamic ramping, which means the temperature gradient forms during ramping and all wells come to the designated temperature at the same time. As a result, the incubation period is the same for all samples, which is crucial for transfer of gradient to nongradient protocols.
The O-ring hermetic seal extends the life of the heating and cooling elements. Condensation from constant heating and cooling can degrade thermal electric modules (TEs), causing them to fail. Bio-Rad’s 1000-series cyclers include a patented* O-ring seal (red in illustration) that forms an airtight barrier around the TEs, preventing exposure to condensation. This substantially lengthens the life of TEs and guarantees outstanding thermal performance.
* U.S. patent 7,051,536.
The Ultimate in Configuration Flexibility
Bio-Rad's 1000-series thermal cycling platform offers maximum flexibility, so you can build a thermal cycling system that meets all your needs.
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![]() USB flash drive |
![]() CFX96 optical reaction module |
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![]() 96–deep well reaction module, gradient enabled |
![]() Dual 48/48-well fast reaction module, gradient enabled |
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![]() 384-well reaction module, gradient enabled |
Configuration Options
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Stand-Alone
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Stand-Alone Multi-Instrument
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Software-Controlled Stand-Alone
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Software-Controlled Multi-Instrument
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With the Protocol Autowriter, Protocol Setup Is As Easy As 1, 2, 3 Using the right cycling protocol can have a great impact on the efficiency of your PCR. The C1000 Touch™ thermal cycler's protocol autowriter sets a new standard for easy programming by generating a customized temperature protocol based on your enzyme type, PCR product length, and primer annealing temperature. Using established PCR guidelines, the protocol autowriter suggests a cycling protocol with hot start, initial denaturation, annealing/extension steps, and final extension. It can even create a fast protocol for you, so you can get results faster. |
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1. Enter the polymerase, amplicon length, and primer annealing temperature (Ta). |

Protocols generated by the protocol autowriter at various standard (S), fast (F), and ultrafast (U) settings yield comparable results. 2 kb, 10 kb, and 20 kb fragments were amplified from a DNA template by iProof DNA polymerase, and various fragments up to 501 bp in length were amplified from human genomic DNA using iTaq DNA polymerase. Equal amounts of reaction mixture were loaded into the well for each set of amplicons. M, markers.
Compatibility of PCR Plastic Consumables with Bio-Rad Thermal Cyclers
Thermal Cyclers | ||||
![]() MJ Mini™ Gradient Thermal Cycler |
![]() T100™ Thermal Cycler |
![]() DNA Engine® Thermal Cycler Family |
![]() C1000 Touch™/S1000™ Thermal Cyclers |
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Tubes | ||||
0.5 ml individual tubes | • | • | Deep well module only |
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0.2 ml individual tubes | • | • | • | • |
High-profile tube strips | • | • | • | • |
Low-profile tube strips | • | • | • | |
48-Well and 96-Well Plates | ||||
Hard-Shell® low-profile 96-well semi-skirted PCR plates | • | |||
Hard-Shell high-profile 96-well semi-skirted PCR plates | • | • | • | |
Hard-Shell low-profile 96-well skirted PCR plates | • | • | ||
Multiplate™ high-profile unskirted PCR plates | • | • | • | • |
Multiplate low-profile unskirted PCR plates | • | • | • | |
iQ™ 96-well semi-skirted PCR plates | • | • | • | |
384-Well Plates | ||||
Hard-Shell 384-well skirted PCR plates | • | • | ||
Lids | ||||
Domed cap strips | • | • | • | • |
Optical flat cap strips | • | • | • | • |
Adhesive Seals | ||||
Microseal® 'A' film | • | • | • | |
Microseal 'B' adhesive seals | • | • | • | |
Microseal ꞌCꞌ optical seals | • | • | • | |
Microseal 'F' foil | • | • | • | |
Heat Sealer and Heat Sealing Films and Foils | ||||
PX1™ PCR plate sealer | • | • | • | |
Optically clear heat seal | • | • | • | |
Pierceable foil heat seal | • | • | • | |
Peelable foil heat seal | • | • | • |
The C1000 Touch PCR thermal cycler offers superior performance and a color touch screen for easy programming. It can accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds.
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