CFX384 Touch Real-Time PCR Detection System

The CFX384 Touch Real-Time PCR System brings flexibility and ease of use to researchers performing precise, high-throughput real-time PCR applications in a 384-well format.

Ordering Information

Overview

Product Discontinuation

The CFX96 Touch and CFX384 Touch Real-Time PCR Detection Systems will be discontinued soon. Please see the CFX Opus Real-Time PCR System for alternative products. If you are interested in purchasing a CFX Touch unit, complete this form, and one of our sales representatives will contact you.

The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

Key Features and Benefits

With the CFX384 Touch Real-Time PCR Detection System you can:

Obtain precise quantification and multiplex target discrimination – optical system uses long-lasting solid-state technology with 5 filtered LEDs and 5 filtered photodiodes
Configure the system to fit your laboratory needs – optimized design lets you run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
Confirm system performance and data reliability – internal system qualification and system test software, automated data quality control, and various validation tool services are available
Obtain accurate results with volumes as low as 3 µl – minimize sample and reagent usage
Easily integrate with your laboratory information management system (LIMS) – integrate with any LIMS using built-in LIMS file management
Combine the CFX384 Touch System with good laboratory practice standards – use CFX Maestro Software, Security Edition, which complies with U.S. FDA 21 CFR Part 11 regulations, for data collection and analysis

Flexible CFX Systems Configurations Meet Your Throughput Needs

The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

Flexible Configurations Meet Your Throughput Needs

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

Begin analyzing data in a single step by importing target, reference and control information from a PrimePCR run file directly into CFX Maestro Software
Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments

The Bio-Rad Expert Care Service Program offers various service plans to keep your systems functioning optimally
CFX System IQ/OQ Services and CFX Automation System II IQ/OQ Services certify system installation and validate system performance
Thermal Validation Services test temperature accuracy and uniformity
The CFX Qualification Plate quickly and easily verifies the performance of your CFX Real-Time PCR Detection Systems

Related Technologies

Related Products

Specifications

Thermal Cycler

Chassis

C1000 Touch™

Maximum ramp rate, °C/sec

2.5

Average ramp rate, °C/sec

Heating and cooling method

Peltier

Lid, °C

Heats up to 105

Temperature

Range, °C

0–100

Accuracy, °C

±0.2 of programmed target at 90°C

Uniformity, °C

±0.4 well-to-well within 10 sec of arrival at 90°C

Gradient

Operational range, °C

30–100

Programmable span, °C

1–24

Optical Detection

Excitation

5 filtered LEDs

Detection

5 filtered photodiodes

Range of excitation/emission wavelengths, nm

450–690

Sensitivity

Detects 1 copy of target sequence in human genomic DNA

Dynamic range

10 orders of magnitude

Scan Time

All channels, sec

<20

FAM/SYBR^® Green only, sec

Software

Operating systems

Windows 7, Windows 8, Windows 10

Multiplex analysis

Up to 4 targets per well

System

Licensed for real-time PCR

Yes

Sample capacity, wells

384

Sample size, µl

1–30 (5–20 recommended)

Communication interface

USB 2.0

Electrical approvals

IEC, CE

Dimensions (W x D x H)、cm/in

33 x 46 x 36/13 x 18 x 14

Weight, kg/lb

21/47

Ordering

items

Use the filters below to refine results!

(Discontinued) CFX384 Touch Real-Time PCR Detection System with Starter Package

1855484

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler Chassis, CFX384 Optical Reaction Module, CFX Maestro Software, qbase+ Software license, cables, reagents, consumables

(Discontinued) CFX384™ Optical Reaction Module for Real-Time PCR Systems with Starter Package

1845384

384-well optical module for real-time PCR, includes CFX Maestro™ Software, qbase+ Software license, cables, reagents, consumables, for use with C1000 Touch™ Thermal Cycler Chassis

Accessories

(Discontinued) CFX Qualification Plate

1845099

Predispensed 384-well plate for performance validation of CFX384™ or CFX384 Touch™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water

(Discontinued) PX1 PCR Plate Sealer

1814000

PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

Kits & Reagents

(Discontinued) SsoAdvanced™ Universal SYBR^® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725271

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl₂, SYBR^® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions

(Discontinued) SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725281

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl₂, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions

(Discontinued) iTaq™ Universal SYBR^® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725131

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, enhancers, stabilizers, and ROX Normalization Dyes

(Discontinued) SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml

1725160

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

(Discontinued) SingleShot™ SYBR^® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725085

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR^® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

(Discontinued) iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns

1725038

100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase

(Discontinued) iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl

1708841

100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl₂, stabilizers; 50 reactions of no-RT control supermix

Plastics

(Discontinued) Hard-Shell® 384-Well PCR Plates, thin wall, skirted, black/white

HSP3865

Pkg of 50, black shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

(Discontinued) Hard-Shell® 384-Well PCR Plates, thin wall, skirted, clear/white, barcoded

HSP3905

Pkg of 50, clear shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

(Discontinued) Hard-Shell® 384-Well PCR Plates, thin wall, skirted, clear/white

HSP3805

Pkg of 50, clear shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

Downloads

Precision Melt Analysis™ v1.2 Software Upgrade

SOFT-PM-12-PATCH

Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager Software version 2.0 and higher

Download Software

Software Download

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