CFX384 Touch Real-Time PCR Detection System

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Overview

The CFX384 Touch Real-Time PCR System brings flexibility and ease of use to researchers performing precise, high-throughput real-time PCR applications in a 384-well format.

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Description

Description

CFX System

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CFX System

Real-Time PCR Tips, Tricks, and Best Practices Guide

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The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

Key Features and Benefits

With the CFX384 Touch Real-Time PCR Detection System you can:

  • Obtain precise quantification and multiplex target discrimination – optical system uses long-lasting solid-state technology with 5 filtered LEDs and 5 filtered photodiodes
  • Configure the system to fit your laboratory needs – optimized design lets you run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
  • Confirm system performance and data reliability – internal system qualification and system test software, automated data quality control, and various validation tool services are available
  • Obtain accurate results with volumes as low as 3 µl – minimize sample and reagent usage
  • Easily integrate with your laboratory information management system (LIMS) – integrate with any LIMS using built-in LIMS file management
  • Combine the CFX384 Touch System with good laboratory practice standards – use CFX Maestro Software, Security Edition, which complies with U.S. FDA 21 CFR Part 11 regulations, for data collection and analysis

Flexible CFX Systems Configurations Meet Your Throughput Needs

The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

Flexible Configurations Meet Your Throughput Needs


CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

  • Begin analyzing data in a single step by importing target, reference and control information from a PrimePCR run file directly into CFX Maestro Software
  • Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
  • Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments

Specifications

Specifications

Thermal Cycler
 
Chassis
C1000 Touch™
Maximum ramp rate, °C/sec
2.5
Average ramp rate, °C/sec
2
Heating and cooling method
Peltier
Lid, °C
Heats up to 105
Temperature
 
Range, °C
0–100
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
 
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
 
Excitation
5 filtered LEDs
Detection
5 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–690
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range 
10 orders of magnitude
Scan Time
 
All channels, sec
<20
FAM/SYBR® Green only, sec
8
Software
 
Operating systems
Windows 7, Windows 8, Windows 10
Multiplex analysis
Up to 4 targets per well
System
 
Licensed for real-time PCR
Yes
Sample capacity, wells
384
Sample size, µl
1–30 (5–20 recommended)
Communication interface
USB 2.0
Electrical approvals
IEC, CE
寸法 (W x D x H)、cm (インチ)
33 x 46 x 36/13 x 18 x 14
Weight, kg/lb
21/47

Ordering

Ordering

items

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1855484
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler Chassis, CFX384 Optical Reaction Module, CFX Maestro Software, qbase+ Software license, cables, reagents, consumables
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1845384
384-well optical module for real-time PCR, includes CFX Maestro™ Software, qbase+ Software license, cables, reagents, consumables, for use with C1000 Touch™ Thermal Cycler Chassis

Accessories

Accessories

cfx-validation-plate-SKU-184-5099-thumb.jpg
1845099
Predispensed 384-well plate for performance validation of CFX384™ or CFX384 Touch™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water
PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
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1841001
Kit contains mouse, mouse pad, USB flash drive

Kits & Reagents

Kits & Reagents

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1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
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1725281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
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1725121
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes
172-5131_tn.jpg
1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes
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1725160

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

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1725085
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
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1725038
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase
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1708841
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

Plastics

Plastics

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MSB1001
Pkg of 100, optically clear seal for PCR plates
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HSP3865
Pkg of 50, black shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP3905
Pkg of 50, clear shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design
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HSP3805
Pkg of 50, clear shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

Download

Download

Precision Melt Analysis™ v1.2 Software Upgrade
SOFT-PM-12-PATCH
Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager™ Software version 2.0 and higher

Documents

Documents

Number Description Options
7444
A Model qPCR Instrument Comparability Study: CFX96 Touch and CFX Opus 96 Real-Time PCR Systems
7180
CFX96 Touch Protein Thermal Shift Assay Protocol
6894
Tips, Tricks & Best Practices: The Ultimate qPCR Assay Design Guide
[ Add to Cart (Free) ]
6096
CFX384 Touch Real-Time PCR Detection System Brochure
[ Add to Cart (Free) ]
10000068706
C1000 Touch™ Thermal Cycler and CFX Real-Time PCR Detection Systems Instrument Guide, Ver A
6900
CFX Maestro Software Brochure
[ Add to Cart (Free) ]
10000068703
CFX Maestro Software User Guide
6077
CFX384 Touch Real-Time PCR Detection System Flier
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
10027342
CFX Qualification Plate — 384-Well Plate Format Product Insert
10022527
Amplification Plastics and Reagents Selection Guide
5690
CFX Manager™ Software, Security Edition Product Information Sheet, Rev A
10000115384
Checking Firmware and Software Updates for the C1000 Touch Thermal Cycler and the CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems, Quick Guide, Ver D
7216
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR
5962
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A
6047
Optical Design of CFX96™ Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A
6144
Plate Preparation for the CFX384™ Real-Time PCR Detection System Using a Tecan Robotics System, Rev A
6149
Plate Preparation for the CFX384™ Real-Time PCR Detection System Using the IDEX Health & Science Innovadyne Dispenser, Rev A
5692
Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A
5279
Real-Time PCR Applications Guide, Rev B
5990
Gene Expression Analysis Solutions Brochure
[ Add to Cart (Free) ]
6634
A Fast Semi-Automated Quantitative PCR Method for Monitoring Differential Gene Expression for Drug Target Discovery, Ver B
6629
High-Throughput Real-Time PCR Solutions Brochure
6777
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR