SsoAdvanced™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725271 2 , SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
1725271
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml
1725271
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02/27/23 11:15 AM
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1725271-ssoadvanced-universal-sybr-green-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml
1
1725271
SsoAdvanced Universal SYBR Green Supermix
SsoAdvanced™ Universal SYBR<sup>®</sup> Green Supermix, 500 x 20 µl rxns, 5 ml (5
x 1 ml)
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>,
SYBR<sup>®</sup> Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>,
SYBR<sup>®</sup> Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-sybr-green-supermix-172-5271-thumb.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-sybr-green-supermix-172-5271-view.jpg
,VN
Molly Gregg
SsoAdvanced Universal SYBR Green Supermix
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>,
SYBR<sup>®</sup> Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
172-5271, 1725271, ssoadvanced, universal, sybr, green, supermix, super, master, mix,
mastermix, detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase,
rox
02/27/13 11:09 AM
02/27/23 11:15 AM
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
en
LSR
/LSR/Amplification---PCR/PCR-Reagents
N
0
<p>SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time
PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix
is formulated for a wide range of real-time PCR applications and for use with all
ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a
href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>,
stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">superior
inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>,
specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">sensitivity</a>,
efficiency, or reproducibility.</p>
<p>SsoAdvanced Universal SYBR Green Supermix lets you:</p>
<ul>
<li><strong>Use any real-time PCR system</strong> — the universal reference
dye in this supermix enables ROX normalization of qPCR data regardless of the ROX
level requirements of the qPCR system</li>
<li><strong>Achieve superior real-time PCR results under various conditions</strong>
— our robust formulation delivers consistent performance in fast cycling across
a broad range of reaction conditions, primer concentrations, and temperature ranges</li>
<li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised
samples</strong> — the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">increased
resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">better
sensitivity</a> and overall performance</li>
<li><strong>Decrease time to results without compromising qPCR data quality</strong>
— the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization
kinetics and instant polymerase activation</li>
<li><strong>Obtain better results with Bio-Rad's <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance-tutorial.html');">PrimePCR™
assays</a></strong> — real-time PCR assays are expertly designed and wet-lab
validated to ensure optimal assay performance</li>
</ul>
<h2 class="descHeader">Instrument Compatibility</h2>
<p>SsoAdvanced Universal SYBR Green Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad
Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td colspan="2" valign="middle"><strong>Systems</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Applied Biosystems</td>
<td valign="top">
<ul>
<li>StepOne/StepOnePlus</li>
<li>7500, ViiA 7</li>
<li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">Stratagene</td>
<td valign="top">
<ul>
<li>Mx3000P, 3005P, 4000</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Eppendorf</td>
<td valign="top">
<ul>
<li>Mastercycler ep realplex 2, 4</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">QIAGEN</td>
<td valign="top">
<ul>
<li>Corbett Rotor-Gene 3000, 6000, Q</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Roche</td>
<td valign="top">
<ul>
<li>LightCycler 480, 96</li>
<li>LightCycler 1.0, 1.5, 2.0*</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">BioFire</td>
<td valign="top">
<ul>
<li>LightScanner HR-1</li>
<li>LightScanner 32*</li>
</ul>
</td>
</tr>
</tbody>
</table>
<p class="caption caption-margin">* BSA must be added according to instrument specifications</p>
<h2 class="descHeader">Applications and Uses of SsoAdvanced Universal SYBR Green
Supermix</h2>
<ul>
<li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li>
<li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li>
<li><a href="/en-us/prime-pcr-assays/pathway/primepcr-pathways">Pathway analysis</a></li>
<li>Absolute quantification</li>
<li>Chromatin immunoprecipitation (ChIP) qPCR</li>
<li>Mutation detection</li>
<li>Pathogen detection</li>
<li>Viral detection (load)</li>
<li>Characterization of genetically modified organisms (GMO)</li>
<li>Genetic profiling</li>
</ul>
<h2 class="descHeader">Packaging Options</h2>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td><strong>Number of 20 μl Reactions</strong></td>
<td><strong>Total Volume</strong></td>
<td><strong>Number of Vials</strong></td>
<td><strong>Catalog #</strong></td>
</tr>
<tr class="pd_colorbackground">
<td>200</td>
<td>2 ml</td>
<td>2 x 1 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5270">1725270</a></td>
</tr>
<tr>
<td>500</td>
<td>5 ml</td>
<td>5 x 1 ml</td>
<td>1725271</td>
</tr>
<tr class="pd_colorbackground">
<td>1,000</td>
<td>10 ml</td>
<td>10 x 1 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5272">1725272</a></td>
</tr>
<tr>
<td>2,500</td>
<td>25 ml</td>
<td>5 x 5 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5274">1725274</a></td>
</tr>
<tr class="pd_colorbackground">
<td>5,000</td>
<td>50 ml</td>
<td>10 x 5 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5275">1725275</a></td>
</tr>
</tbody>
</table>
<h2 class="descHeader">Related Products</h2>
<p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H424VY">SsoAdvanced
universal probes supermix</a>, which is available in several formats (200–5,000
reactions).</p>
<p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p>
<ul>
<li><a href="/en-us/category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA
isolation kits</a></li>
<li><a href="/en-us/category/cdna-synthesis-kits?ID=M87EPGE8Z">Reverse transcription
kits</a></li>
<li><a href="/en-us/category/real-time-pcr-supermixes-kits?ID=M87F1J8UU">Real-time
PCR supermixes</a></li>
</ul>
<h2 class="descHeader">More Information</h2>
<p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step
<a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization
protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis
of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf">troubleshooting
reactions</a>.</p>
<p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p>
/webroot/web/html/lsr/products/SKUs
172-5271.html
/webroot/web/html/lsr/products/SKUs/172-5271.html
Product expiration (after shipping date)
2026
12 months
1
0
Storage at 4°C (after thawing)
2806
Up to 3 months
1
0
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2530
12 months
1
0
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Product detail page
1725271
List Price:
$399.00
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1725271
399.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
SsoAdvanced™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725281 2 , stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
1725281
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml
1725281
02/28/13 05:07 PM
en_US
true
internet/product_SKU
2019-05-04 16:19:19
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templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml
/default/main/bio-rad/WORKAREA/default
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LSR
N
/default/main/bio-rad
en
02/28/23 05:08 PM
1555974593493
2019-05-04 16:19:19
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1725281-ssoadvanced-universal-probes-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml
1
1725281
SsoAdvanced Universal Probes Supermix
SsoAdvanced™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>,
stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>,
stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-probes-supermix-172-5281-thumb.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-probes-supermix-172-5281-view.jpg
,VN
Molly Gregg
SsoAdvanced Universal Probes Supermix
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>,
stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
172-5281, 1725281, ssoadvanced, universal, probes, supermix, super, master, mix, mastermix,
detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase, rox
02/28/13 05:07 PM
02/28/23 05:08 PM
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
en
LSR
/LSR/Amplification---PCR/PCR-Reagents
N
0
<p>SsoAdvanced Universal Probes Supermix is a high-performance real-time PCR supermix
based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated
for a wide range of real-time PCR applications and for use with all ROX dependent
or independent real-time PCR systems. The dsDNA binding protein, <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>,
stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html');">superior
inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>,
specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html');">sensitivity</a>,
efficiency, or reproducibility.</p>
<p>SsoAdvanced universal probes supermix lets you:</p>
<ul>
<li><strong>Carry out high-performance singleplex and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/duplex-reaction.html');">duplex</a>
reactions</strong> — the Sso7d fusion polymerase and advanced formulation enable
<a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/long-amplicon-gc-rich-duplex-reaction.html');">robust
performance</a> in singleplex or duplex real-time PCR reactions, providing the highest
data precision and allowing cost and time savings when combining 2 assays in a single
well</li>
<li><strong>Use any real-time PCR system</strong> — the universal reference
dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless
of the ROX level requirements of the qPCR system</li>
<li><strong>Achieve superior real-time PCR results under various conditions</strong>
— our robust formulation delivers consistent performance in fast cycling across
a broad range of reaction conditions, primer concentrations, and temperature ranges</li>
<li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised
samples</strong> — the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html');">increased
resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html');">better
sensitivity</a> and overall performance</li>
<li><strong>Decrease time to results without compromising qPCR data quality</strong>
— the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization
kinetics and instant polymerase activation</li>
</ul>
<h2 class="descHeader">Instrument Compatibility</h2>
<p>SsoAdvanced Universal Probes Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad
Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td colspan="2" valign="middle"><strong>Systems</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Applied Biosystems</td>
<td valign="top">
<ul>
<li>StepOne/StepOnePlus</li>
<li>7500, ViiA 7</li>
<li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">Stratagene</td>
<td valign="top">
<ul>
<li>Mx3000P, 3005P, 4000</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Eppendorf</td>
<td valign="top">
<ul>
<li>Mastercycler ep realplex 2, 4</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">QIAGEN</td>
<td valign="top">
<ul>
<li>Corbett Rotor-Gene 3000, 6000, Q</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Roche</td>
<td valign="top">
<ul>
<li>LightCycler 480, 96</li>
<li>LightCycler 1.0, 1.5, 2.0*</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">BioFire</td>
<td valign="top">
<ul>
<li>LightScanner HR-1</li>
<li>LightScanner 32*</li>
</ul>
</td>
</tr>
</tbody>
</table>
<p class="caption caption-margin">* BSA must be added according to instrument specifications</p>
<h2 class="descHeader">Applications and Uses of SsoAdvanced Universal Probes Supermix</h2>
<ul>
<li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li>
<li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li>
<li>Absolute quantification</li>
<li>Genotyping (allelic discrimination)</li>
<li>Mutation detection</li>
<li>Pathogen detection</li>
<li>Viral detection (load)</li>
<li>Characterization of genetically modified organisms (GMO)</li>
<li>Genetic profiling</li>
</ul>
<h2 class="descHeader">Packaging Options</h2>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td><strong>Number of 20 μl Reactions</strong></td>
<td><strong>Total Volume</strong></td>
<td><strong>Number of Vials</strong></td>
<td><strong>Catalog #</strong></td>
</tr>
<tr class="pd_colorbackground">
<td>200</td>
<td>2 ml</td>
<td>2 x 1 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5280">1725280</a></td>
</tr>
<tr>
<td>500</td>
<td>5 ml</td>
<td>5 x 1 ml</td>
<td>1725281</td>
</tr>
<tr class="pd_colorbackground">
<td>1,000</td>
<td>10 ml</td>
<td>10 x 1 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5282">1725282</a></td>
</tr>
<tr>
<td>2,500</td>
<td>25 ml</td>
<td>5 x 5 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5284">1725284</a></td>
</tr>
<tr class="pd_colorbackground">
<td>5,000</td>
<td>50 ml</td>
<td>10 x 5 ml</td>
<td><a href="/evportal/destination/commerce/sku_detail?productID=172-5285">1725285</a></td>
</tr>
</tbody>
</table>
<h2 class="descHeader">Related Products</h2>
<p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H1EE8Z">SsoAdvanced™
Universal SYBR<sup>®</sup> Green Supermix</a>, which is available in several formats
(200–5,000 reactions).</p>
<p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p>
<ul>
<li><a href="/evportal/destination/product?catID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA
isolation kits</a></li>
<li><a href="/evportal/destination/product?catID=M87EPGE8Z">Reverse transcription
kits</a></li>
<li><a href="/evportal/destination/product?catID=M87F1J8UU">Real-time PCR supermixes</a></li>
</ul>
<h2 class="descHeader">More Information</h2>
<p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step
<a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization
protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis
of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/bulletin-10031340.pdf">troubleshooting
reactions</a>.</p>
<p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p>
/webroot/web/html/lsr/products/SKUs
172-5281.html
/webroot/web/html/lsr/products/SKUs/172-5281.html
Product expiration (after shipping date)
2026
12 months
1
0
Storage at 4°C (after thawing)
2806
Up to 3 months
1
0
Customer guarantee (minimum usage time) at –20°C
2530
12 months
1
0
false
$388.00
false
$
null
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/prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725281&AddToDefaultWishList=true&quantity=
41106300
388.00
Product detail page
1725281
List Price:
$388.00
Loading
EDU Price:
What is this?
Your Price:
Loading
Your Price:
Quantity: 1725281
Add to Cart
1725281
388.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
iTaq™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725121 2 , SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes
1725121
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml
1725121
02/28/12 03:32 PM
en_US
true
internet/product_SKU
2019-05-04 16:19:09
/default/main/bio-rad/STAGING
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml
/default/main/bio-rad/WORKAREA/default
jayharva
2017-11-14 07:07:36
true
jayharva
templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml
LSR
N
/default/main/bio-rad
en
02/28/22 03:37 PM
1555974591683
2019-05-04 16:19:09
/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml
1725121-itaq-universal-sybr-green-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml
1
1725121
iTaq Universal SYBR Green Supermix
iTaq™ Universal SYBR<sup>®</sup> Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase,
MgCl<sub>2</sub>, SYBR<sup>®</sup> Green I, enhancers, stabilizers, fluorescein,
and ROX Normalization Dyes
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq; DNA Polymerase,
MgCl<sub>2</sub>, SYBR<sup>®</sup> Green I, enhancers, stabilizers, fluorescein,
and ROX Normalization Dyes
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5121_tn.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5121_view.jpg
,VN
Karen Moss
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq™
DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>®</sup> Green I, enhancers, stabilizers,
fluorescein, and ROX Normalization Dyes
172-5121, 1725121, itaq, universal, sybr, green, supermix, super, master, mix, mastermix,
detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, dna, hot-start,
hot, start, polymerase, mgcl2, fluorescein, rox
02/28/12 03:32 PM
02/28/22 03:37 PM
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
en
LSR
/LSR/Amplification---PCR/PCR-Reagents
N
0
<p>Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive
reference dyes for dye-based quantitative PCR (qPCR).</p>
<ul>
<li>Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument</li>
<li>Contains both ROX and fluorescein dyes for universal real-time instrument compatibility
(ROX-independent and ROX-dependent systems)</li>
<li>Amplify genomic DNA and difficult amplicons</li>
<li>Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)</li>
</ul>
<h2 class="descHeader">Instrument Compatibility</h2>
<p>iTaq Universal SYBR Green Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad
Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td colspan="2" valign="middle"><strong>Systems</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Applied Biosystems</td>
<td valign="top">
<ul>
<li>StepOne/StepOnePlus</li>
<li>7500, ViiA 7</li>
<li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">Stratagene</td>
<td valign="top">
<ul>
<li>Mx3000P, 3005P, 4000</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Eppendorf</td>
<td valign="top">
<ul>
<li>Mastercycler ep realplex 2, 4</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">QIAGEN</td>
<td valign="top">
<ul>
<li>Corbett Rotor-Gene 3000, 6000, Q</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Roche</td>
<td valign="top">
<ul>
<li>LightCycler 480, 96</li>
<li>LightCycler 1.0, 1.5, 2.0*</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">BioFire</td>
<td valign="top">
<ul>
<li>LightScanner HR-1</li>
<li>LightScanner 32*</li>
</ul>
</td>
</tr>
</tbody>
</table>
<p class="caption caption-margin">* BSA must be added according to instrument specifications</p>
<h2 class="descHeader">Packaging Options</h2>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td valign="top"><strong>Number of 20 µl Reactions</strong></td>
<td valign="top"><strong>Supermix Volume, ml</strong></td>
<td valign="top"><strong>Catalog Number</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">200</td>
<td valign="top">2 x 1</td>
<td valign="top"><a href="/en-us/sku/1725120-itaq-universal-sybr-green-supermix"><span>1725120</span></a></td>
</tr>
<tr>
<td valign="top">500</td>
<td valign="top">5 x 1</td>
<td valign="top"><span>1725121</span></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">1,000</td>
<td valign="top">10 x 1</td>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5122">1725122</a></td>
</tr>
<tr>
<td valign="top">2,500</td>
<td valign="top">5 x 5</td>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5124">1725124</a></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">5,000</td>
<td valign="top">10 x 5</td>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5125">1725125</a></td>
</tr>
</tbody>
</table>
<p>See the <a href="/en-us/product/itaq-universal-supermixes/itaq-universal-sybr-green-supermix">iTaq
Universal SYBR Green Supermix</a> product page for more information and documentation.</p>
<h2 class="descHeader">Related Products</h2>
<ul>
<li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time
PCR Supermixes and Kits</a></li>
<li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR™ PCR Primers
& Assays</a></li>
</ul>
<h2 class="descHeader">Related Categories</h2>
<ul>
<li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li>
<li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection
Systems</a></li>
<li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li>
<li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument
Validation Tools and Services</a></li>
</ul>
<h2 class="descHeader">Supporting Documents</h2>
<ul>
<li>iTaq Universal SYBR Green Supermix <a href="/webroot/web/pdf/lsr/literature/10041157.pdf">Product
Insert</a></li>
<li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection
Guide</a></li>
</ul>
<h2 class="descHeader">Online Resources</h2>
<ul>
<li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li>
<li><a href="/en-us/applications-technologies/genomics">Genomics</a></li>
</ul>
/webroot/web/html/lsr/products/SKUs
172-5121_desc.html
/webroot/web/html/lsr/products/SKUs/172-5121_desc.html
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/prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725121&CartQuote=QT&quantity=
1458
false
true
false
/prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725121&AddToDefaultWishList=true&quantity=
41106300
322.00
Product detail page
1725121
List Price:
$322.00
Loading
EDU Price:
What is this?
Your Price:
Loading
Your Price:
Quantity: 1725121
Add to Cart
1725121
322.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725131 2 , enhancers, stabilizers, and ROX Normalization Dyes
1725131
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5131_1330479375.xml
1725131
02/28/12 05:28 PM
en_US
true
internet/product_SKU
2019-05-04 16:19:11
/default/main/bio-rad/STAGING
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5131_1330479375.xml
/default/main/bio-rad/WORKAREA/default
jcihuc4i
2017-11-14 07:07:36
true
jcihuc4i
templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5131_1330479375.xml
LSR
N
/default/main/bio-rad
en
02/28/22 05:30 PM
1555974591895
2019-05-04 16:19:11
/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5131_1330479375.xml
1725131-itaq-universal-probes-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml
1
1725131
iTaq Universal Probes Supermix
iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA
Polymerase, MgCl<sub>2</sub>, enhancers, stabilizers, and ROX Normalization Dyes
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA
Polymerase, MgCl<sub>2</sub>, enhancers, stabilizers, and ROX Normalization Dyes
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5131_tn.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5131_view.jpg
,VN
Karen Moss
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA
Polymerase, MgCl<sub>2</sub>, enhancers, stabilizers, and ROX Normalization Dyes
172-5131, 1725131, itaq, universal, probes, supermix, super, mix, master, mastermix,
detection, amplification, real-time, pcr, real, time, qpcr, gene, expression, quantification,
quantitative, rox, hot-start, hot, start
02/28/12 05:28 PM
02/28/22 05:30 PM
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
en
LSR
/LSR/Amplification---PCR/PCR-Reagents
N
0
<p>Use the iTaq Universal Probes Supermix for probe-based quantitative PCR (qPCR;
simplex or duplex reaction) on any real-time PCR instrument.</p>
<ul>
<li>Reliable and reproducible results with any fluorogenic probe</li>
<li>Blend of passive dyes, including ROX</li>
<li>5 x 1 ml (enough for 500 x 20 µl reactions)</li>
<li>Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)</li>
</ul>
<h2 class="descHeader">Instrument Compatibility</h2>
<p>iTaq Universal Probes Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad
Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td colspan="2" valign="middle"><strong>Systems</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Applied Biosystems</td>
<td valign="top">
<ul>
<li>StepOne/StepOnePlus</li>
<li>7500, ViiA 7</li>
<li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">Stratagene</td>
<td valign="top">
<ul>
<li>Mx3000P, 3005P, 4000</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Eppendorf</td>
<td valign="top">
<ul>
<li>Mastercycler ep realplex 2, 4</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">QIAGEN</td>
<td valign="top">
<ul>
<li>Corbett Rotor-Gene 3000, 6000, Q</li>
</ul>
</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">Roche</td>
<td valign="top">
<ul>
<li>LightCycler 480, 96</li>
<li>LightCycler 1.0, 1.5, 2.0*</li>
</ul>
</td>
</tr>
<tr>
<td valign="top">BioFire</td>
<td valign="top">
<ul>
<li>LightScanner HR-1</li>
<li>LightScanner 32*</li>
</ul>
</td>
</tr>
</tbody>
</table>
<p class="caption caption-margin">* BSA must be added according to instrument specifications</p>
<h2 class="descHeader">Packaging Options</h2>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td valign="top"><strong>Number of 20 µl Reactions</strong></td>
<td valign="top"><strong>Supermix Volume, ml</strong></td>
<td valign="top"><strong>Catalog Number</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">200</td>
<td valign="top">2 x 1</td>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5130">1725130</a></td>
</tr>
<tr>
<td valign="top">500</td>
<td valign="top">5 x 1</td>
<td valign="top">1725131</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">1,000</td>
<td valign="top">10 x 1</td>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5132">1725132</a></td>
</tr>
<tr>
<td valign="top">2,500</td>
<td valign="top">5 x 5</td>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5134">1725134</a></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">5,000</td>
<td valign="top">10 x 5</td>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5135">1725135</a></td>
</tr>
</tbody>
</table>
<h2 class="descHeader">Related Product</h2>
<p><a href="/en-us/product/itaq-universal-supermixes/itaq-universal-sybr-green-supermix">iTaq
Universal SYBR<sup>®</sup> Green Supermix:</a> optimized for qPCR on any real-time
PCR instrument, with reference dyes ROX and fluorescein</p>
<h2 class="descHeader">More Information</h2>
<p>Learn about the <a href="/en-us/product/itaq-universal-supermixes/itaq-universal-probes-supermix">key
features and specifications of the iTaq Universal Probes Supermix.</a></p>
/webroot/web/html/lsr/products/SKUs/autopopulated
171-5131_desc.html
/webroot/web/html/lsr/products/SKUs/autopopulated/171-5131_desc.html
1
0
false
$322.00
false
$
null
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true
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Product detail page
1725131
List Price:
$322.00
Loading
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Your Price:
Quantity: 1725131
Add to Cart
1725131
322.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml
1725160
1725160
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml
1725160
11/22/13 09:02 AM
en_US
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internet/product_SKU
2019-05-04 16:19:24
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1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml
1
1725160
SsoAdvanced PreAmp Supermix
SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml
1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts,
enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions
1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts,
enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-preamp-supermix-172-5160-thumb.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-preamp-supermix-172-5160.jpg
SsoAdvanced PreAmp Supermix
preview
,VN
Karen Moss
SsoAdvanced PreAmp Supermix
1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase
(patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 µl reactions
1725160, ssoadvanced, preamp, supermix, super, master, mix, mastermix, detection,
amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase,
mgcl2
11/22/13 09:02 AM
11/22/23 09:07 AM
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<p>Use the <strong>SsoAdvanced PreAmp Supermix</strong> to achieve the unbiased preamplification
of up to 100 qPCR target sequences in a single reaction.</p>
<ul>
<li>Start with as little as 100 pg of cDNA — amplify precious samples like
stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded
tissues (FFPE)</li>
<li>Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan
Gene Expression Assays</li>
<li>For gene expression and genotyping applications — validated with <a href="/en-us/product/pcr-primers-probes-panels">PrimePCR™
PreAmp Assays</a>, self-designed assays, and TaqMan Assays</li>
</ul>
<h2 class="descHeader">Product Comparison</h2>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td><strong>Product</strong></td>
<td><strong>ΔΔCq*</strong></td>
<td><strong>Chemistry</strong></td>
<td><strong>Reactions</strong></td>
<td><strong>Price**</strong></td>
</tr>
<tr class="pd_colorbackground">
<td>SsoAdvanced PreAmp Supermix</td>
<td>90% within ± 0.75</td>
<td>SYBR<sup>®</sup> Green and probes</td>
<td>50 x 50 µl</td>
<td>Least expensive</td>
</tr>
<tr>
<td>TaqMan PreAmp Master Mix</td>
<td>90% within ± 1.5</td>
<td>Probes only</td>
<td>40 x 50 µl</td>
<td>Most expensive</td>
</tr>
<tr class="pd_colorbackground">
<td>PerfeCTa PreAmp 5x Supermix</td>
<td>90% within ± 1.5</td>
<td>SYBR<sup>®</sup> Green and probes</td>
<td>40 x 50 µl</td>
<td>More expensive</td>
</tr>
</tbody>
</table>
<br />
<p class="caption" style="margin-left: 13px;">* Relative quantification between nonamplified
and preamplified cDNA.<br /> ** As of May 2014.</p>
<h2 class="descHeader">Simple Workflow</h2>
<p>Setting up a preamplification takes only a few minutes. Simply combine the pooled
assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start
the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting
template is complete and ready for downstream analyses.</p>
<p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/simple-workflow-ssoadvanced-preamp-supermix-5-12.jpg"
alt="Simple Workflow for SsoAdvanced PreAmp Supermix" width="597" height="278" /></p>
<h2 class="descHeader">Recommended RT-qPCR Reagents and Assays</h2>
<p>Reverse Transcription</p>
<ul>
<li><a href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript™
Reverse Transcription Supermix for RT-qPCR</a></li>
<li><a href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript™
Advanced cDNA Synthesis Kit for RT-qPCR</a></li>
</ul>
<p>qPCR Supermixes</p>
<ul>
<li><a href="/en-us/product/ssoadvanced-universal-sybr-green-supermix">SsoAdvanced™
Universal SYBR<sup>®</sup> Green Supermix</a></li>
<li><a href="/en-us/product/ssoadvanced-universal-probes-supermix">SsoAdvanced™
Universal Probes Supermix</a></li>
</ul>
<p>PreAmp and qPCR Assays</p>
<ul>
<li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR™ PreAmp Assays</a></li>
<li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR™ Assays</a></li>
</ul>
<h2 class="descHeader">More Information</h2>
<p>Find out more about the features of the <a href="/en-us/product/ssoadvanced-preamp-supermix">SsoAdvanced™
PreAmp Supermix</a>.</p>
/webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global
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1725271
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1725281
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41106300
727.00
Product detail page
1725160
List Price:
$727.00
Loading
EDU Price:
What is this?
Your Price:
Loading
Your Price:
Quantity: 1725160
Add to Cart
1725160
727.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions
1725085 ® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
1725085
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5085_1401922795.xml
1725085
06/04/14 02:40 PM
en_US
true
internet/product_SKU
2019-05-04 16:19:29
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LSR
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/default/main/bio-rad
en
06/04/24 03:59 PM
1555974590805
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1725085-singleshot-sybr-green-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions
1
1725085
SingleShotSYBRGreenKit
SingleShot™ SYBR<sup>®</sup> Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA
Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR<sup>®</sup> Green
Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA
Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR<sup>®</sup> Green
Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/singleshot-sybr-green-kit-172-5085-thumb.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/singleshot-sybr-green-kit-172-5085.jpg
preview
,VN
Karen Moss
SingleShot™ SYBR® Green Cell Lysis and RT-qPCR Kit
SingleShot™ SYBR<sup>®</sup> Green Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions
1725085, singleshot, sybr, green, cell, lysis, reagents, iscript, cdna, synthesis,
supermix, one, step, rt-qpcr, reverse, transcription, quantitative, pcr, detection,
amplification
06/04/14 02:40 PM
06/04/24 03:59 PM
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
en
LSR
/LSR/Amplification---PCR/PCR-Reagents
N
0
<p>Use the <strong>SingleShot™ SYBR<sup>®</sup> Green Kit</strong> to obtain
high-quality RT-qPCR data with SYBR<sup>®</sup> Green chemistry directly from
cell culture lysates.</p>
<p>Two-step workflow provides results less than 2 hours after cell lysis. All RT-qPCR
reagents and RNA control included.</p>
<p>Benefits include:</p>
<ul>
<li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li>
<li>Complete <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/genomic-dna-removal-from-cell-lysates.html');">removal
of genomic DNA</a> while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving
RNA integrity</a></li>
<li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/reproducibility-of-technical-lysate.html');">Optimal
accuracy</a> and linear dynamic range of qPCR data across genes</li>
<li>Results comparable to using purified RNA, including dynamic range</li>
<li>Validated with PrimePCR™ Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li>
<li>SingleShot RNA Control ensures optimal cell and lysate inputs</li>
</ul>
<h2 class="descHeader">Optimized Sample Input</h2>
<p>The SingleShot™ SYBR<sup>®</sup> Green Kit maximizes cell lysate and
cDNA inputs in a two-step RT-qPCR reaction:</p>
<table class="pd_table pd_gridlines" style="width: 500px;" border="0">
<tbody>
<tr>
<td>Maximum cell lysate into RT reaction</td>
<td>45% of total volume</td>
</tr>
<tr class="pd_colorbackground">
<td>Maximum undiluted cDNA into qPCR reaction</td>
<td>20% of total volume</td>
</tr>
</tbody>
</table>
<br />
<h2 class="descHeader">Kit Contents</h2>
<table class="pd_table" style="width: 500px;" border="0">
<tbody>
<tr>
<td width="310" align="left" valign="top">
<p><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer
<br /> SingleShot Proteinase K Solution <br /> SingleShot DNase Solution</p>
</td>
<td width="190" align="left" valign="top">
<p><strong>100 x 50 μl reactions</strong><br /> 5 ml (1 x 5 ml vial)<br />100 μl
(1 x 1 ml vial)<br />100 μl (1 x 1 ml vial)</p>
</td>
</tr>
</tbody>
</table>
<table class="pd_table" style="width: 500px;" border="0">
<tbody>
<tr>
<td width="310" align="left" valign="top">
<p><strong>cDNA Synthesis Kit</strong><br /> 5x iScript Advanced Reaction Mix<br />
iScript Advanced Reverse Transcriptase<br /> Nuclease-Free Water</p>
</td>
<td width="190" align="left" valign="top">
<p><strong>100 reactions</strong><br /> 400 μl (1 x 1 ml vial)<br /> 100 μl
(1 x 1 ml vial)<br /> 1 ml (1 x 1 ml vial)</p>
</td>
</tr>
</tbody>
</table>
<table class="pd_table" style="width: 500px;" border="0">
<tbody>
<tr>
<td width="310" align="left" valign="top">
<p><strong>qPCR Supermix</strong><br /> SsoAdvanced Universal SYBR<sup>®</sup>
Green Supermix</p>
</td>
<td width="190" align="left" valign="top">
<p><strong> 500 reactions</strong><br /> 5 ml (5 x 1 ml vials)</p>
</td>
</tr>
</tbody>
</table>
<table class="pd_table" style="width: 500px;" border="0">
<tbody>
<tr>
<td width="310" align="left" valign="top">
<p><strong>SingleShot RNA Control </strong><br /> SingleShot RNA Control Template
<br /> SingleShot™ SYBR<sup>®</sup> Green qPCR Control Assay</p>
</td>
<td width="190" align="left" valign="top">
<p><strong> 200 reactions</strong><br /> Lyophilized (200 reactions)<br />200 μl
(1 x 1 ml vial)</p>
</td>
</tr>
</tbody>
</table>
<p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042474.pdf"
target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction
setup and troubleshooting tips.</p>
<h2 class="descHeader">Cell Lines Validated for Use with the SingleShot™ SYBR<sup>®</sup>
Green Kit</h2>
<p>The SingleShot™ SYBR<sup>®</sup> Green Kit has been validated for use
with a number of common human and rodent cell lines, such as all types of adherent
and suspension cells, including but not limited to:</p>
<table class="pd_table pd_gridlines" style="width: 500px;" border="0">
<tbody>
<tr>
<td width="75"><strong>Cell Line</strong></td>
<td width="88"><strong>Property</strong></td>
<td width="323"><strong> Species</strong></td>
</tr>
<tr class="pd_colorbackground">
<td>A549</td>
<td>Adherent </td>
<td><em>H. sapiens</em>, lung carcinoma</td>
</tr>
<tr>
<td>HeLa</td>
<td>Adherent</td>
<td><em>H. sapiens</em>, cervical adenocarcinoma</td>
</tr>
<tr class="pd_colorbackground">
<td>HepG2 </td>
<td>Adherent </td>
<td><em>H. sapiens</em>, liver carcinoma</td>
</tr>
<tr>
<td>NIH/3T3</td>
<td>Adherent</td>
<td><em>M. musculus</em>, embryonic fibroblast</td>
</tr>
<tr class="pd_colorbackground">
<td>MCF-7</td>
<td>Adherent </td>
<td><em>H. sapiens</em>, epithelial, adenocarcinoma</td>
</tr>
<tr>
<td>NCI-H1975</td>
<td>Adherent</td>
<td><em>H. sapiens</em>, lung adenocarcinoma </td>
</tr>
<tr class="pd_colorbackground">
<td>K-562</td>
<td>Suspension</td>
<td><em>H. sapiens</em>, chronic myelogenous leukemia</td>
</tr>
<tr>
<td>HEK 293</td>
<td>Suspension</td>
<td><em>H. sapiens</em>, kidney</td>
</tr>
<tr class="pd_colorbackground">
<td>Jurkat</td>
<td>Suspension</td>
<td><em>H. sapiens</em>, T lymphocyte</td>
</tr>
<tr>
<td>PC-12 </td>
<td>Suspension</td>
<td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td>
</tr>
<tr class="pd_colorbackground">
<td>HepRG </td>
<td>Primary-like</td>
<td><em>H. sapiens</em>, hepatocytes</td>
</tr>
</tbody>
</table>
<h2 class="descHeader">Related Products</h2>
<p>The SingleShot™ SYBR<sup>®</sup> Green Kit belongs to the family of <a
href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot™ Cell Lysis RT-qPCR
Kits</a>:</p>
<ul>
<li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> — including
SingleShot™ SYBR<sup>®</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5095">172-5095</a>)
and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5070">172-5070</a>)</li>
<li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> — including SingleShot™
SYBR<sup>®</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5085">172-5085</a>)
and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5090">172-5090</a>)</li>
<li><strong>SingleShot Cell Lysis Kit</strong> — available for 100 reactions
(<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&vertical=LSR&productID=172-5080">172-5080</a>)</li>
</ul>
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Storage at –20°C
2199
<strong>12 months:</strong><br /> Lysis buffer, DNase, and proteinase K solution<br
/> iScript Advanced cDNA Synthesis Kit<br /> SsoAdvanced™ Universal SYBR<sup>®</sup>
Green Supermix <br /> SingleShot RNA Control Template<br /> SingleShot™
SYBR<sup>®</sup> Green qPCR Control Assay
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Product detail page
1725085
List Price:
$1,278.00
Loading
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Your Price:
Quantity: 1725085
Add to Cart
1725085
1278.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns
1725038
1725038
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5038_1403297461.xml
1725038
06/20/14 01:43 PM
en_US
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2019-05-04 16:19:16
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06/20/24 01:45 PM
1555974590596
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1725038-iscript-advanced-cdna-synthesis-kit-100-x-20-ul-rxns
1
1725038
iScriptAdvancedcDNASynthesisKitforRT-qPCR
iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript
Advanced Reverse Transcriptase
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript
Advanced Reverse Transcriptase
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8842_tn.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8842_view.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8842_feature.jpg
iScript Advanced cDNA Synthesis Kit for RT-qPCR
preview
,VN
Dave Lubertozzi
1725038, iscript, advanced, cdna, synthesis, rt-qpcr, 100x20, reactions, reverse,
transcription, first, strand, complimentary, gene, expression, real-time, real, time,
pcr, qpcr, detection
06/20/14 01:43 PM
06/20/24 01:45 PM
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
en
LSR
/LSR/Amplification---PCR/PCR-Reagents
N
0
<p>Use the iScript Advanced cDNA Synthesis Kit for RT-qPCR for increased data throughput
from a single 20 µl reverse transcription (RT) reaction for real-time qPCR.
Two-tube kit allows up to 7.5 µg input RNA.</p>
<ul>
<li>Ability to use higher input RNA amounts to analyze a large number of target genes</li>
<li>Detect low-level target genes — use with less input RNA, or when RNA sample
is limited</li>
<li>Reduce inter-assay variability and improve reproducibility — eliminate the
need for multiple cDNA synthesis reactions</li>
<li>Prevent 5′ and 3′ bias — optimum blend of oligo(dT) and random
primers</li>
<li>2-tube kit generates cDNA in 21 min</li>
</ul>
<h2 class="descHeader">Packaging Options</h2>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td align="left"><strong>Kit Size</strong></td>
<td align="left"><strong>Catalog Number</strong></td>
</tr>
<tr class="pd_colorbackground">
<td align="left" valign="top">25 x 20 µl reactions</td>
<td align="left" valign="top"><a href="/en-us/sku/172-5037-iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">1725037</a></td>
</tr>
<tr>
<td align="left" valign="top">100 x 20 µl reactions</td>
<td align="left" valign="top">1725038</td>
</tr>
<tr class="pd_colorbackground">
<td align="left" valign="top">500 x 20 µl reactions</td>
<td align="left" valign="top"><a href="/en-us/sku/1725038bun-iscript-advanced-cdna-synthesis-kit-500-x-20-ul-rxns?ID=1725038BUN">1725038BUN</a></td>
</tr>
</tbody>
</table>
<br />
<h2 class="descHeader">Related Products</h2>
<ul>
<li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li>
<li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes
and Kits</a></li>
<li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR™ PCR Primers
& Assays</a></li>
</ul>
<h2 class="descHeader">Related Categories</h2>
<ul>
<li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li>
<li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection
Systems</a></li>
<li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li>
<li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument
Validation Tools and Services</a></li>
</ul>
<h2 class="descHeader">More Information</h2>
<p>Learn about the <a href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr?tab=Description">key
features and specifications of the iScript Advanced cDNA Synthesis Kit for RT-qPCR.</a></p>
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172-5038-1.html
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41106300
656.00
Product detail page
1725038
List Price:
$656.00
Loading
EDU Price:
What is this?
Your Price:
Loading
Your Price:
Quantity: 1725038
Add to Cart
1725038
656.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl
1708841 2 , stabilizers; 50 reactions of no-RT control supermix
1708841
/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml
1708841
09/09/10 02:10 PM
en_US
true
internet/product_SKU
2020-07-10 17:39:25
/default/main/bio-rad/STAGING
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
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jcihuc4t
2017-11-14 07:07:35
true
jcihuc4t
templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml
LSR
N
/default/main/bio-rad
en
12/31/37 11:59 PM
1556988417516
2020-07-10 17:39:25
/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml
1708841-iscript-reverse-transcription-supermix-100-x-20-ul-rxns-400-ul
1
1708841
iScript Reverse Transcription Supermix for RT-qPCR
iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase,
RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 50 reactions of no-RT
control supermix
/webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8841_tn.jpg
/webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8841_view.jpg
,VN
Rick Abruzzo
iScript™ Reverse Transcription Supermix for RT-qPCR
100 x 20 µl reactions, 4 x 100 µl 5x supermix contains reverse transcriptase,
RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 50 reactions of no-RT
control supermix
170-8841, 1708841, iscript, reverse, transcription, supermix, rt, qpcr, super, master,
mix, mastermix, cdna, synthesis, real-time, real, time, pcr, amplification, transcriptase,
rt-pcr
09/09/10 02:10 PM
12/31/37 11:59 PM
AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN
en
LSR
/LSR/Amplification---PCR/PCR-Reagents
N
0
<p>Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of
first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains
all the components for reverse transcription except RNA template.</p>
<h2 class="descHeader">Key Features and Benefits</h2>
<ul>
<li>Combined oligo(dT) and random primers provide unbiased representation of 5' and
3' regions</li>
<li>Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1
pg total input RNA</li>
<li>Amplify cDNA for genes with low expression levels</li>
<li>Fast 26-minute 1-tube cDNA synthesis protocol</li>
<li>50 reactions of no-RT controls for optional detection of gDNA amplicon contamination</li>
</ul>
<h2 class="descHeader">Packaging Options</h2>
<table class="pd_table pd_gridlines" border="0">
<tbody>
<tr>
<td valign="top"><strong>Kit Contents</strong></td>
<td valign="top"><strong>Supermix Volume, µl</strong></td>
<td valign="top"><strong>Catalog Number</strong></td>
</tr>
<tr class="pd_colorbackground">
<td>25 x 20 µl reactions</td>
<td valign="top"> 100 (1 x 100 µl)</td>
<td valign="top"><a href="/en-us/sku/1708840-iscript-reverse-transcription-supermix-25-x-20-ul-rxns-100-ul">1708840</a></td>
</tr>
<tr>
<td>100 x 20 µl reactions</td>
<td valign="top"> 400 (4 x 100 µl)</td>
<td valign="top">1708841</td>
</tr>
<tr class="pd_colorbackground">
<td>500 x 20 µl reactions</td>
<td>2000 (20 x 100 µl)</td>
<td><a href="/en-us/sku/1708841bun-iscript-reverse-transcription-supermix-500-x-20-ul-rxns-2-ml?ID=1708841BUN">1708841BUN</a></td>
</tr>
</tbody>
</table>
<br />
<p>See the <a href="/en-us/product/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript
Reverse Transcription Supermix</a> product page for more information and documentation.</p>
<h2 class="descHeader">Related Products</h2>
<ul>
<li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li>
<li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes
and Kits</a></li>
<li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR™ PCR Primers
& Assays</a></li>
</ul>
<h2 class="descHeader">Related Categories</h2>
<ul>
<li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li>
<li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection
Systems</a></li>
<li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li>
<li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument
Validation Tools and Services</a></li>
</ul>
<h2 class="descHeader">Supporting Documents</h2>
<ul>
<li>iScript Reverse Transcription Supermix <a href="/webroot/web/pdf/lsr/literature/10020178.pdf"
target="_blank" rel="noopener noreferrer">Product Insert</a></li>
<li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf"
target="_blank" rel="noopener noreferrer">Selection Guide</a></li>
</ul>
<h2 class="descHeader">Online Resources</h2>
<ul>
<li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li>
<li><a href="/en-us/applications-technologies/genomics">Genomics</a></li>
</ul>
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iscript-reverse-transcription-supermix-for-rt-qpcr-170-8841-desc.html
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31141500
546.00
Product detail page
1708841
List Price:
$546.00
Loading
EDU Price:
What is this?
Your Price:
Loading
Your Price:
Quantity: 1708841
Add to Cart
1708841
546.00
The minimum orderable quantity of this product is 1
Educational (EDU) Pricing
For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).
If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number.
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