CFX96 Touch Real-Time PCR Detection System

CFX96 Touch Real-Time PCR Detection System

The CFX96 Optical Reaction Module converts a C1000 Touch Thermal Cycler into a real-time PCR system. This six-channel system combines advanced optical technology with precise thermal control.

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Overview

Product Discontinuation

The CFX96 Touch and CFX384 Touch Real-Time PCR Detection Systems will be discontinued soon. Please see the CFX Opus Real-Time PCR System for alternative products. If you are interested in purchasing a CFX Touch unit, complete this form, and one of our sales representatives will contact you. We will continue to provide support for the CFX96 Touch and CFX384 Real-Time PCR Detection Systems until December 2026.

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CFX System

Real-Time PCR Tips, Tricks, and Best Practices Guide

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The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.

Key Features and Benefits

With the CFX96 Touch Real-Time PCR Detection System you can:

  • Set up your system quickly — easy installation and factory-calibrated optics
  • Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
  • Optimize reactions in a single run — thermal gradient feature
  • Analyze data faster — visualize all run data at once and export only the data you need in the format you want
  • Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
  • Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
  • Combine the CFX96 Touch System with good laboratory practice standards — use CFX Maestro Software, Security Edition for data collection and analysis to simplify compliance with U.S. FDA 21 CFR Part 11 regulations

Flexible CFX System Configurations Meet Your Throughput Needs

The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

Flexible Configurations Meet Your Throughput Needs

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

  • Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
  • Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
  • Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments

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Specifications

Thermal Cycler
 
Chassis
C1000 Touch™
Maximum ramp rate, °C/sec
5
Average ramp rate, °C/sec
3.3
Heating and cooling method
Peltier
Lid, °C
Heats up to 105
Temperature
 
Range, °C
0–100
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
 
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
 
Excitation
6 filtered LEDs
Detection
6 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–730
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Scan Time
 
All channels, sec
12
FAM/SYBR® Green only, sec
3
Software
 
Operating systems
Windows 7, Windows 8, Windows 10
Multiplex analysis
Up to 5 targets per well
System
 
Licensed for real-time PCR
Yes
Sample capacity, wells
96
Sample size, µl
1–50 (10–25 recommended)
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x D x H)、cm/in
33 x 46 x 36 /13 x 18 x 14
Weight, kg/lb
21/47

Ordering

items

Use the filters below to refine results!

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(Discontinued) CFX96 Optical Reaction Module for Real-Time PCR Systems with Starter Package
1845096
96–well optical module for real-time PCR, includes CFX Maestro Software, qbase+ Software license, cables, reagents, consumables, for use with C1000 Touch™ Thermal Cycler Chassis
184-5096_tn.jpg
(Discontinued) CFX96 Optical Reaction Module for Real-Time PCR Systems
1845097
96-well optical module for real-time PCR, includes cables, for use with C1000 Touch Thermal Cycler Chassis

Accessories

cfx-validation-plate-SKU-184-5098-thumb.jpg
(Discontinued) CFX Qualification Plate
1845098
Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water
PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

Kits & Reagents

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(Discontinued) SsoAdvanced™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
ssoadvanced-universal-probes-supermix-172-5281-thumb.jpg
(Discontinued) SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions
172-5121_tn.jpg
(Discontinued) iTaq™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

172-5131_tn.jpg
(Discontinued) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes
ssoadvanced-preamp-supermix-172-5160-thumb.jpg
(Discontinued) SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml
1725160

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

singleshot-sybr-green-kit-172-5085-thumb.jpg
(Discontinued) SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions
1725085

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

170-8842_tn.jpg
(Discontinued) iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns
1725038
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase
170-8841_tn.jpg
(Discontinued) iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl
1708841
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

Plastics

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(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear
HSP9601

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

HSP9901_tn.jpg
(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded
HSP9901

Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

HSP9655_tn.jpg
(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white
HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
multiplate-low-profile-96-well-unskirted-pcr-plates-mll-9601-thumb.jpg
(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear
MLL9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples
mll-9651_tn.jpg
(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, white
MLL9651
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
tls-0801_tn.jpg
(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, clear
TLS0801

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

tls-0851_tn.jpg
(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, white
TLS0851

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

TCS0803 TN
(Discontinued) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear
TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

Download

CFX96™ and CFX384™ Real-Time PCR Detection Systems Firmware Update
SOFT-CFX-FW
Ensures optimal performance from your instrument. Version HC12 1.0.176, PXA270 3.3.155.524, DSP 1.124, and 8051 Smartlid 0.54
Precision Melt Analysis™ v1.2 Software Upgrade
SOFT-PM-12-PATCH
Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager™ Software version 2.0 and higher
64-Bit OS PC Patch
SOFT-64-PATCH
Patch installs Windows 64-bit operating system PC drivers compatible with CFX96™ and CFX384™ real-time PCR detection systems controlled by CFX Manager™ software

Documents

Number Description Options
7444
A Model qPCR System Comparability Study: CFX Touch and CFX Opus Real-Time PCR Platforms
7180
CFX Real-Time PCR Systems Protein Thermal Shift Assay Protocol
6093
CFX96 Touch™ Real-Time PCR Detection System Brochure
[ Add to Cart (Free) ]
6894
Tips, Tricks & Best Practices: The Ultimate qPCR Assay Design Guide
[ Add to Cart (Free) ]
10000068706
C1000 Touch™ Thermal Cycler and CFX Real-Time PCR Detection Systems Instrument Guide, Ver A
6900
CFX Maestro Software Brochure
[ Add to Cart (Free) ]
10000068703
CFX Maestro Software User Guide
10000115381
CFX96 Touch and CFX96 Touch Deep Well Real-Time PCR Detection Systems Installation Quick Guide, Ver D
10027341
CFX Qualification Plate — 96-Well Plate Format Product Insert
10000115384
Checking Firmware and Software Updates for the C1000 Touch Thermal Cycler and the CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems, Quick Guide, Ver D
6144
Plate Preparation for the CFX384™ Real-Time PCR Detection System Using a Tecan Robotics System, Rev A
6149
Plate Preparation for the CFX384™ Real-Time PCR Detection System Using the IDEX Health & Science Innovadyne Dispenser, Rev A
5690
CFX Manager™ Software, Security Edition Product Information Sheet, Rev A
10022527
Amplification Plastics and Reagents Selection Guide
5990
Gene Expression Analysis Solutions Brochure
[ Add to Cart (Free) ]
6629
High-Throughput Real-Time PCR Solutions Brochure
5279
Real-Time PCR Applications Guide, Rev B
7216
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR
6047
Optical Design of CFX96™ Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A
5692
Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A
5962
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A
6634
A Fast Semi-Automated Quantitative PCR Method for Monitoring Differential Gene Expression for Drug Target Discovery, Ver B
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
6777
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR