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Overview
200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard
Safety Data Sheet
Description
Description
iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.
Features and Benefits of iProof High-Fidelity DNA Polymerase:
- High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
- Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
- Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)
iProof Polymerase is available in an easy-to-use master mix and a PCR kit complete with controls.
| iProof High-Fidelity DNA Polymerase | Number of Reactions | Catalog Number |
| iProof High-Fidelity PCR Kit | 50 U (2 U/µl) | 1725330 |
| iProof High-Fidelity PCR Kit | 200 U (2 U/µl) | 1725331 |
| iProof HF Master Mix | 100 x 50 µl reactions | 1725310 |
| iProof GC Master Mix | 100 x 50 µl reactions | 1725320 |
| iProof High-Fidelity DNA Polymerase | 20 U (2 U/µl) | 1725300 |
| iProof High-Fidelity DNA Polymerase | 100 U (2 U/µl) | 1725301 |
| iProof High-Fidelity DNA Polymerase | 500 U (2 U/µl) | 1725302 |
Related Categories
Supporting Documentation
- iProof High-Fidelity PCR Kit Instruction Manual
- iProof High-Fidelity Master Mix Instruction Manual
- Amplification Reagents and Plastics Brochure
Recommended SKUs
Recommended SKUs
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1708841
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix