Bio-Plex Multiplex Immunoassays
Maximize insights, minimize sample — Bio-Rad Multiplex Immunoassays with Luminex xMAP® Technology
Accelerate Research with Luminex Multiplex Assays
Bio-Rad’s Bio-Plex Multiplex Immunoassays powered by Luminex® xMAP® Technology enable researchers to simultaneously detect and quantify multiple analytes — such as cytokines, chemokines, growth factors, and biomarkers — in a single, 12.5µl sample. Bio-Plex Luminex assays are designed to deliver precise, reproducible, and actionable data for a wide range of applications, including immunology, oncology, infectious disease, autoimmune, diabetes, and cell signaling research.
Multiplex Assay Kits
Select complete kits for multiplex target panels
Singleplex Assays
Select individual target analytes of interest
Custom Bio-Plex Assays
Design your own custom-mixed assays from over 450 available analytes
Why Choose Bio-Plex Luminex Multiplex Immunoassays?
- Efficiency: Simultaneous measurement up to 48 analytes per sample, saving time, and resources
- Data Quality: Reliable and reproducible results for low-abundance targets
- Comprehensive Solutions: Ready-to-use kits and customizable panels
- Broad Spectrum: Assays for cytokines, chemokines, growth factors, and more, for human, mouse, and rat species
- Advanced Technology: Achieve high-resolution data with Luminex® xMAP® Technology
- Expert Support: Dedicated technical experts and customer support
- Proven Results: Trusted by researchers worldwide for reliable data
How Luminex xMAP® Technology Works
Bio-Rad’s Bio-Plex multiplex immunoassays are powered by Luminex® xMAP® technology — a bead-based platform that enables multiplex analysis of up to 48 analytes per well. Each Bio-Plex assay uses fluorescently color-coded magnetic beads, each coupled with a unique antibody to capture specific analytes from a biological sample. After sample incubation:
- Detection antibodies are added to form a sandwich complex
- Streptavidin-phycoerythrin (SA-PE) provides the fluorescent signal
- The sample is analyzed using a Bio-Plex system or any Luminex system, which uses lasers to:
- Identify each bead (analyte identification)
- Quantify fluorescence intensity (analyte concentration)
This powerful system maximizes insights, minimizes sample — ideal for cytokines, chemokines, growth factors, and more.
Bio-Plex Assays & Kits
Browse by Panel
Human
Multi-Species
Mouse
Rat
Search by Target
Simply select your targets of interest to find the assay that best meets your needs.
- Filter by analyte and species: Human, Mouse, or Rat
- Email search results
- Contact us for help finding the right assays for your needs
Custom Bio-Plex Assays
Bio-Plex Assay Builder
Design your own custom-mixed assays from our growing selection of over 450 available analytes using our interactive tool.
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Express Assays
Pick the targets and get everything you need to mix custom assays yourself
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x-Plex Assays
Pick the targets and we mix ready-to-use custom assays for you
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Get answers to your questions about Premade Bio-Plex Assays and Kits
Bio-Plex Instruments & Software
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Bio-Plex Instruments
Bio-Plex Readers and Tools include the Bio-Plex 200 and Bio-Plex 3D Systems, Bio-Plex Pro Wash Station, and maintenance, calibration, verification, and validation kits.
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Bio-Plex Software
Bio-Plex Manager and Bio-Plex Data Pro Software make it simple to run instruments using xMAP technology and analyze multiplex data.
Assay Development Reagents & Accessories
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Bio-Plex Amine Coupling Kit
The Bio-Plex Amine Coupling Kit was developed specifically for immobilizing antibodies or other proteins to carboxylated beads. For use in sandwich, indirect and competitive assays. Can be used to couple Luminex xMAP® microspheres.
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Bio-Plex Streptavidin-PE
Bio-Plex Streptavidin-Phycoerythrin is included in Bio-Plex reagent kits and is also available separately to enable flexibility during assay development and optimization.
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16-Tube SureBeads™ Magnetic Rack
The magnetic rack uses high-strength magnets for rapid separation of suspended magnetic beads. Holds 16 x 1.5 ml tubes or 2 x 15 ml tubes.
Resources
Bio-Plex Multiplex Immunoassay Manuals, Guides & Protocols Library
Find protocols for all the Bio-Plex assay groups, Application Notes, Multiplex Immunoassays Success Guides for sample prep and hardware & software use.
Luminex xMAP Powered Bio-Plex Multiplex Immunoassays
Learn about the early immunoassays and principles of bead-based mulitiplex immunoassays used in the Bio-Plex systems powered by Luminex xMAP Technology.
Multiplex Immunoassay Design and Validation
Learn the details of assay design, assay quality, and performance definitions.
Advantages of Multiplex Immunoassays
See how our SMARTS assay development process yields high-performing xMAP multiplex immunoassays with high sensitivity and broad dynamic range to give you faster and more reproducible results with shorter incubation times.
Bio-Plex Blog on Bioradiations
Read the latest application notes for Bio-Plex Luminex assays from Bio-Rad R&D scientists and see what other researchers do with Bio-Plex Multiplex Assays.
Bio-Rad Academy: xMAP Bead-based Immunoassays
Learn about immunoassays and xMAP technology in this 6-course series structured to gradually progress from understanding the basics of immunoassays to understanding xMAP technology, running xMAP bead-based assays, and data analysis on the Bio-Plex 200 Manager Software.
Multiplex Immunoassay Success Webinars
Join Bio-Rad multiplex immunodetection experts for a webinar series that explores the many factors that go into the design and execution of successful xMAP Multiplex Immunoassays.
96-Well Plate Template Post-It Pad
Simplifies the Bio-Plex Multiplex Assay process from start to finish.
Bio-Plex Quick Tip Videos
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Planning for Assay Success Part 1: Setup & Samples
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Planning for Assay Success Part 2: Instrument Prep
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Planning for Assay Success Part 3: Best Practices
Bio-Plex Assays Applications
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Bio-Plex System Applications & Uses
Watch short videos to learn how the Bio-Plex System works, how it is used in cardiovascular disease and leukemia research, biomarker identification, and cytokine expression profiling in cancer studies.
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Bench to Bedside
Learn how Bio-Plex assays and instruments are being used in cardiovascular disease and leukemia research.
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Understanding Melanoma Biomarkers
Learn how the Bio-Plex system can help to identify early markers of melanoma in serum and blood samples from at-risk patients.
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Understanding Cancer
Learn how Bio-Plex assays and instruments are being used to monitor cytokine expression in cancer and in response to cancer vaccines.
Bio-Plex Luminex Assays vs. ELISA
Comparison of Bio-Plex Multiplexing vs. Traditional ELISA
Protocol comparison between traditional ELISA and the Bio-Plex System. The Bio-Plex Multiplex Immunoassay System, using Luminex xMAP technology, offers high-performance readers, industry-leading software, and sensitive assays in ready-to-use or custom configurations, enabling you to quantify over 48 different protein and peptide targets simultaneously in a single 12.5 µl sample.
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Bio-Plex Suspension Array System: xMAP® Technology Overview
Luminex xMAP® technology enables multiplex quantitation of 100 different analytes in solution using fluorescent detection of unique capturing bead sets.
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Bio-Plex Multiplex Immunoassays vs. ELISA in the Clinic
Clinical Study — Bio-Plex Cytokine Immunoassays and ELISA: A Comparison of Two Methodologies in Testing Samples from Asthmatic and Healthy Children
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Overcoming the Limitations of ELISA with the Bio-Plex Multiplex Reader
xMAP® technology offers all the benefits of the ELISA workflow plus higher throughput, increased flexibility, reduced sample size, and lower costs.
