Human Diabetes Assays

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Overview

Multiplex immunoassays built on magnetic beads for quantifying multiple diabetes protein biomarkers. Available as a 10-plex panel, custom Express assays, and singleplex sets.

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Description

Description

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Bio-Plex Assay Ordering Options

Bio-Plex Kits

Multiplex Assay Kits
Select complete kits for multiplex target panels

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Bio-Plex Singleplex Assays

Singleplex Assays
Select individual target analytes of interest.

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Bio-Plex Pro human diabetes immunoassays offer reliable performance and a convenient format for studying 12 markers for human diabetes and obesity.

These assays are magnetic bead–based assays, available as either premixed panels or singleplex sets, that rapidly (three hr) detect multiple diabetes and obesity biomarkers in a single experiment, using as little as 12.5 µl of sample. The 10-plex assay detects:

  • C-peptide
  • Ghrelin
  • GIP
  • GLP-1
  • Glucagon
  • Insulin
  • Leptin
  • PAI-1 (total)
  • Resistin
  • Visfatin

Additionally, adiponectin and adipsin can also be assayed; they are available as either singleplexes or a 2-plex. Due to different dilution schemes, these biomarkers should be assayed separately from the ten markers in the panel.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.


Key Features

  • Magnetic beads for simplified plate processing
  • Results obtained in 3 hr due to short 1 hr incubation time
  • All-in-one, space-saving complete kit
  • Premixed standards for generation of curves over a broad dynamic range for each target

Other Assay Formats Available

  • Custom assays (mixed at the bench): available as Bio-Plex® Express assays
  • Singleplex sets: each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (171304070) and a vial of standards (171D70001) for a complete assay

Instrument Compatibility

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

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Specifications

Specifications

Incubation time, hr
1
Sample volume, µl
12.5
Accuracy (recovery range)
80–120%
Cross-reactivity
Negligible

Ordering

Ordering

items

Use the filters below to refine results!

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171A7001M

1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, control, buffers, diluents, streptavidin-PE, flat bottom plate, sealing tape, for the detection of 10 human diabetes biomarkers

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171A7002M

1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, control, buffers, diluents, streptavidin-PE, flat bottom plate, sealing tape, for the detection of human adiponectin and adipsin

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171A7003M

1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, control, buffers, diluents, streptavidin-PE, flat bottom plate, sealing tape, for the detection of human adiponectin

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171A7004M

1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, control, buffers, diluents, streptavidin-PE, flat bottom plate, sealing tape, for the detection of human adipsin

Accessories

Accessories

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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles

Kits & Reagents

Kits & Reagents

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171304070
1 x 96-well, includes diluents, assay and wash buffers, streptavidin-PE, filter plate, and sealing tape, for use with vacuum separation methods. This reagent kit is being replaced by Bio-Plex Pro Reagent Kit III with Flat Bottom Plate (171304090M)
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171304071
10 x 96-well kit, includes 10 x 96-well filter plate; assay and wash buffers; detection antibody, sample, and standard diluent; streptavidin-PE; sealing tape
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171D70001
Pkg of 1, lyophilized mixture of 12 analytes, enough for 96 assays; for use with Human Diabetes Assays and Reagent Kit III (171304090M)
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171D70050
Pkg of 50, 12 analytes for adiponectin, adipsin, C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, resistin, and visfatin

Singleplexes

Singleplexes

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171B7003M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human C-peptide. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7004M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human ghrelin. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7005M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human GIP. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7006M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human GLP-1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7007M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human glucagon. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7008M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human insulin. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7009M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human leptin. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7010M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human PAI-1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7011M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human resistin. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.
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171B7012M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of human visfatin. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Diabetes Standards (171D70001), which are not included.

Documents

Documents

Number Description Options
10000094511
Bio-Plex Pro Human Diabetes Manual
10000101554
Bio-Plex Pro Human Diabetes Assays Quick Guide
[ Add to Cart (Free) ]
5405
Bio-Plex® Multiplex System Brochure, Ver E
[ Add to Cart (Free) ]
6342
Bio-Plex Pro Diabetes Assay Panels Product Information Sheet
[ Add to Cart (Free) ]
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems