Bio-Plex Pro Mouse Th17 Assays

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Bio-Plex Pro Mouse Th17 Assays

Overview

Bio-Plex Pro Mouse Th17 Assay Specifications
  Assay Working Ranges (pg/ml) Assay Sensitivity (pg/ml) Assay Precision
(%CV)
  Target LLOQ ULOQ LOD Intra-Assay Inter-Assay 
10-Plex IL-17F 25 86,159 8.3 5.9 1.7
IL-21 30 31,793 7.1 4.4 3.2
IL-22 1.0 21,071 0.5 4.7 1.4
IL-23p19 80 273,553 31.8 4.5 1.7
IL-25/IL-17E 5.0 71,463 2.8 2.8 1.4
IL-27 4.0 60,530 0.9 2.7 0.4
IL-31 86 145,2700 36.1 3.0 2.0
IL-33 43 170,779 22.4 3.9 2.7
CD40L 8.0 136,300 4.4 2.8 1.9
MIP-3α 1.0 5,499 0.7 2.7 2.9
 
Singleplex ICAM-1* 81 90,319 14 3.0 5.0
 *Note: the mouse ICAM-1 immunoassay cannot be multiplexed with other assays in the mouse Th17 assay panel due to a different dilution scheme.
6-Plex IL-1α 10.36 60,631 9.4 4 7
IL-6 0.74 12,053 0.2 3 16
IL-10 2.95 12,066 1 4 5
IL-17A 2.65 43,337 0.8 3 10
IFN-γ 1.84 30,164 1.2 4 6
TNF-α 5.8 59,626 1.4 3 6
 
2-Plex IL-25 168 98,266 9 9 12
IL-27p28 63.4 117,521 29.2 9 6

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (limit of detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal

Bio-Plex® Technology and Applications
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Bio-Plex Assay Ordering Options

Multiplex Assay Kits

Select complete kits for multiplex target panels

Bio-Plex Kits

Singleplex Assays

Select individual target analytes of interest.

Bio-Plex Singleplex Assays

The Bio-Plex Pro mouse Th17 magnetic bead–based immunoassays detect and measure the levels of 17 biomarkers for Th17 cells  in diverse matrices such as serum, plasma, and tissue culture supernatants. IL-17 producing T helper cells (Th17 cells) are a new subset of T helper cells recently found to be associated with autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, and lupus. The markers in these assays were selected for their direct relevance to the mouse Th17 pathway.

Based on 6.5 µm magnetic beads, these assays are compatible with magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kits
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target

Flexible Ordering Options

  • Premixed panels — complete ready-to-use kits: Panel A (6-plex), or TGF-β (3-plex)
  • Singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (12002798) for magnetic separation and a vial of standards (171I50001 for group I cytokines, 171IA0001 for Th17 cytokines, or 171X40001 for TGF-β assays) for a complete assay
  • Custom premixed assays — available as Bio-Plex x-Plex assays built using the Bio-Plex Assay Builder
  • Custom assays (mixed at the bench) —  available as Bio-Plex Express assays built using the Bio-Plex Assay Builder

Instrument Compatibility

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Number Description Options
6118 Analysis of Murine Th17 Cytokine Profiles using Bio-Plex Pro Mouse Th17 Panel Click to download [ Add to Cart (Free) ]
5405 Bio-Plex® Multiplex System Brochure, Ver E Click to download [ Add to Cart (Free) ]
6110 Bio-Plex Pro Mouse Th17 Assays Product Information Sheet Click to download
10000111560 Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays Click to download
10000118395 Bio-Plex Pro Mouse Th17 Cytokine Quick Guide Click to download
2993 Bio-Plex Glossary, Rev A Click to download
3100 Bio-Plex Cytokine Assay Comparison, Rev E Click to download
5297 Bio-Plex Pro™ Assay Reference Guide, Ver D Click to download

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