Bio-Plex Pro Human Th17 Cytokine Assays

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Overview

Magnetic bead–based assays for the detection and measurement of 16 human Th17 biomarkers. Available in a premixed, all-in-one 15-plex assay, singleplex sets, or as custom x-Plex and Express Assays.

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Description

Description

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The Bio-Plex Pro Human Th17 Cytokine Panel enables measurement of the following analytes in diverse matrices such as serum, plasma, and cell culture supernatants:

  • IL-1β
  • IL-4
  • lL-6
  • IL-10
  • IL-17A
  • IL-17F
  • IL-17A/F (singleplex only)
  • IL-21
  • IL-22
  • IL-23
  • IL-25
  • IL-31
  • IL-33
  • IFN-γ
  • sCD40L
  • TNF-α

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

The multiplex format enables robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway, which is thought to play a key role in inflammation-associatied conditions such as antimicrobial immunity, cancer, transplant rejection, and autoimmune diseases including rheumatoid arthritis, multiple sclerosis, lupus and psoriasis.

  • Reagent kit ll with diluents optimized for serum and plasma samples
  • Assay quick guide to get you started right away
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
  • 2-level quality controls included with premixed kits

Flexible Ordering Options

Additional Th17 Pathway Assays:

  • TGF-β 3-plex panel (171V4003M)
  • TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires a reagent kit (171304090M) for magnetic separation and a vial of standards (171D60001) for a complete assay

Instrument Compatibility

Compatible with these readers and wash stations:

More Information

See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Related Products

Bio-Plex Pro Human Inflammation Assays
For the first time, the key biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can be measured using a single multiplex kit. Bio-Plex Pro Human Inflammation Assays are available in larger 24-plex and 37-plex screening panels and a pathway-focused Treg 12-plex panel, along with singleplex and custom-configured assays.

Ordering

Ordering

items

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171-AA001M_tn.jpg
171AA001M
1 x 96-well all-in-one kit, includes coupled magnetic beads, detection antibodies, standards, controls, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 15 human Th17 cytokines

Accessories

Accessories

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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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(Discontinued) Filter Plate
171304502
Pkg of 1, 96-well plate, with clear plastic lid and tray, for Bio-Plex Assays using the vacuum wash method, sealing tape not included
171-304500_tn.jpg
171304500
1.5 L, wash buffer for use with Bio-Plex Pro wash stations; not for use with phosphoprotein assays
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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays

Rgnts & Stds

Rgnts & Stds

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171304090M
1 x 96-well, includes detection antibody diluent HB, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape, for magnetic separation methods
171-DA0001_tn.jpg
171DA0001
Pkg of 1 vial, lyophilized mixture of 16 standard antigens, enough for 96 assays; for use with Human Th17 Assays and Reagent Kit III (171304090M)
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171DA0501
Pkg of 50 lot-matched vials, lyophilized mixture of 16 standard antigens, enough for 50 x 96 assays; for use with Human Th17 Assays and Reagent Kit III (171304090M)
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171X40001
Pkg of 1 vial, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)
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171X40501
Pkg of 50 lot-matched vials, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 50 x 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)

Singleplexes

Singleplexes

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171BA001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-1β. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171BA002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-4. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
171-BA003M_tn.jpg
171BA003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human lL-6. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171BA004M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-10. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171BA005M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
171-BA006M_tn.jpg
171BA006M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17F. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
171-BA007M_tn.jpg
171BA007M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-21. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
171-BA008M_tn.jpg
171BA008M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-22. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
171-BA009M_tn.jpg
171BA009M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-23. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
171-BA010M_tn.jpg
171BA010M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-25. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
171-BA011M_tn.jpg
171BA011M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-31. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171BA013M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IFN-γ. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171BA014M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sCD40L. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171BA015M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human TNF-&alpha. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171BA016M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A/F. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.
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171V4001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.
171-v4002m_tn.jpg
171V4002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β2. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.
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171V4003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β3. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.

Documents

Documents

Number Description Options
10023381
Instruction Manual, Bio-Plex Pro™ Human Th17 Cytokine Assays, Rev A
10000080121
Bio-Plex Pro™ Human Th17 Cytokine Assays Quick Guide
6249
Bio-Plex Pro Human Th17 Cytokine Assays Product Information Sheet
6251
Multiplex Analysis of Inflammatory Markers Using Bio-Plex Pro Human Th17 Cytokine Assays, Rev A
5297
Bio-Plex Pro™ Assay Reference Guide, Ver D
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems