Bio-Plex Pro Rat Cytokine, Chemokine, and Growth Factor Assays

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Overview

Magnetic bead–based assays for detecting 28 rat cytokine, chemokine, and growth factor biomarkers. Available as premixed assay panels, x-Plex™ and Express Assays, and as singleplex sets.
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Description

Description

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Bio-Plex Pro Rat Cytokine, Chemokine, and Growth Factor Assays are magnetic bead–based assays to detect and measure levels of 28 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants.

Cytokines, chemokines, and growth factors are cell-signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth and infections to Parkinson's disease.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kits
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
  • Compatible with the new Bio-Plex Pro Rat Diabetes Assays for an expanded biomarker profile

Flexible Ordering Options

  • Premixed panels — complete ready-to-use kits (23-plex, 12-plex, TGF-β 3-plex)
  • Singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires a reagent kit (12002798 for magnetic separation) and a vial of standards (171NZ0001 for rat cytokines or 171X40001 for TGF-β assays) for a complete assay
  • Custom premixed assays — available as Bio-Plex® x-Plex™ Assays built using the Bio-Plex Assay Builder
  • Custom assays (mixed at the bench) —  available as Bio-Plex Express Assays built using the Bio-Plex Assay Builder

Instrument Compatibility

Compatible with these readers and wash stations:

More Information

See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Related Technologies

Ordering

Ordering

items

Use the filters below to refine results!

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12005641
1 x 96-well, includes coupled beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, flat bottom plate, and sealing tape for detecting 23 rat cytokines all in a single well. This product replaces a discontinued assay (171K1001M)
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171K1002M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter and flat bottom plate, and sealing tape for the detection 12 rat cytokines all in one kit
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171W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, and sealing tape for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

Accessories

Accessories

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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
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171304500
1.5 L, wash buffer for use with Bio-Plex Pro wash stations; not for use with phosphoprotein assays
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles

Rgnts & Stds

Rgnts & Stds

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12002798
1 x 96-well, includes mouse HP detection antibody diluent, standard diluent, sample diluent, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape for use with magnetic separation methods
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171NZ0001
Pkg of 1 vial, lyophilized standard for detecting 31 rat targets; compatible with rat cytokine and rat diabetes assays, enough for 96 assays; for use with Rat Cytokine, Chemokine, and Growth Factor Assays or Rat Diabetes Assays and Reagent Kit V (12002798)
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171NZ0501
Pkg of 50 lot-matched vials, lyophilized standard for detecting 31 rat targets; compatible with rat cytokine and rat diabetes assays, enough for 50 x 96 assays; for use with Rat Cytokine, Chemokine, and Growth Factor Assays or Rat Diabetes Assays and Reagent Kit V (12002798)
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171X40001
Pkg of 1 vial, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)
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171X40501
Pkg of 50 lot-matched vials, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 50 x 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)

Singleplexes

Singleplexes

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171L1002M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat EPO, requires reagent kit (#171-304070) and standards
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171L1003M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat G-CSF, requires reagent kit (#171-304070) and standards
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171L1004M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat GM-CSF, requires reagent kit (#171-304070) and standards
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171L1005M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat GRO/KC, requires reagent kit (#171-304070) and standards
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171L1006M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IFN-γ, requires reagent kit (#171-304070) and standards
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171L1007M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-1α, requires reagent kit (#171-304070) and standards
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171L1008M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-1β, requires reagent kit (#171-304070) and standards
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171L1009M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-2, requires reagent kit (#171-304070) and standards
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171L1010M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-4, requires reagent kit (#171-304070) and standards
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171L1011M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-5, requires reagent kit (#171-304070) and standards
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171L1012M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-6, requires reagent kit (#171-304070) and standards
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171L1013M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-7, requires reagent kit (#171-304070) and standards
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171L1014M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-10, requires reagent kit (#171-304070) and standards
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171L1015M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-12p40, requires reagent kit (#171-304070) and standards. Cannot be multiplexed with IL-12p70
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171L1016M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-12p70, requires reagent kit (171-304070) and standards Cannot be multiplexed with IL-12p40
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171L1017M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-13, requires reagent kit (#171-304070) and standards
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171L1018M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat IL-17A, requires reagent kit (#171-304070) and standards
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171L1020M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat M-CSF, requires reagent kit (#171-304070) and standards
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171L1021M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat MIP-1α, requires reagent kit (#171-304070) and standards
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171L1022M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat MIP-2, requires reagent kit (#171-304070) and standards. Cannot be multiplexed with GRO/KC
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171L1023M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat MIP-3α, requires reagent kit (#171-304070) and standards
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171L1024M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat RANTES, requires reagent kit (#171-304070) and standards
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171L1025M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat TNF-α, requires reagent kit (#171-304070) and standards
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171L1026M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat VEGF, requires reagent kit (#171-304070) and standards
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171L1027M
1 x 96-well includes coupled magnetic beads and detection antibodies for detecting rat MCP-1, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-NZ0001)
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171V4001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.
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171V4002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β2. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.
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171V4003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β3. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.

Documents

Documents

Number Description Options
10000111560
Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays
10000076327
Bio-Plex Pro™ Mouse and Rat Cytokine Quick Guide, Ver A
5297
Bio-Plex Pro™ Assay Reference Guide, Ver D
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
5405
Bio-Plex® Multiplex System Brochure, Ver E
[ Add to Cart (Free) ]
6054
Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B
[ Add to Cart (Free) ]
2993
Bio-Plex Glossary, Rev A
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B