Mouse Diabetes Assays

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Overview

Immunoassays for the quantitation of diabetes protein biomarkers in mouse samples are available as a multiplex 11-plex panel, custom Express assays, and singleplex sets.

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Description

Description

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Select individual target analytes of interest.

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Bio-Plex Pro mouse diabetes immunoassays offer reliable performance and a convenient format for studying 9 markers for mouse diabetes and obesity.

These assays are magnetic bead–based assays, available as either premixed panels or singleplex sets, that rapidly (3 hr) detect multiple diabetes and obesity biomarkers in a single experiment, using as little as 12.5 µl of sample.

The 8-plex assay detects:

  • Ghrelin
  • GIP
  • GLP-1
  • Glucagon
  • Insulin
  • Leptin
  • PAI-1 (total)
  • Resistin


Additionally, adiponectin can also be assayed; it is available as a singleplex analyte. Due to different dilution schemes, adiponectin should be assayed separately from the eight markers in the panel.

Based on 6.5 μm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic beads for simplified plate processing
  • Results obtained in 3 hr due to short 1 hr incubation time
  • All-in-one, space-saving complete kit
  • Premixed standards for generation of curves over a broad dynamic range for each target

Other Assay Formats Available

  • Custom assays (mixed at the bench): available as Bio-Plex Express assays
  • Singleplex sets: each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (12002798) and a vial of standards (171I70001) for a complete assay

Instrument Compatibility

Compatible with these readers and wash stations:

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Specifications

Specifications

Incubation time, hr
1
Sample volume, µl
12.5
Accuracy (recovery range)
80–120%
Cross-reactivity
Negligible

Ordering

Ordering

items

Use the filters below to refine results!

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171F7001M
1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, flat bottom plate, sealing tape, for the detection of 8 mouse diabetes biomarkers
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171F7002M
1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, flat bottom plate, sealing tape, for the detection of mouse adiponectin
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171F7010M
Bio-Plex Pro Mouse Diabetes 8-plex Panel, 10x96-well, includes premixed coupled magnetic beads and detection antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, filter plate, sealing tape, standard diluent, sample diluent for the detection of Ghrelin, GIP, GLP-1, Glucagon, Insulin, Leptin, PAI-1, Resistin

Accessories

Accessories

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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles

Kits & Reagents

Kits & Reagents

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12002798
1 x 96-well, includes mouse HP detection antibody diluent, standard diluent, sample diluent, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape for use with magnetic separation methods
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171I70001
Pkg of 1, 9 analytes for adiponectin, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, and resistin
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171I70050
Pkg of 50, 9 analytes for adiponectin, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, and resistin

Singleplexes

Singleplexes

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171G7002M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse ghrelin. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.
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171G7003M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse GIP. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.
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171G7004M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse GLP-1. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.
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171G7005M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse glucagon. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.
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171G7006M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse insulin. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.
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171G7007M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse leptin. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.
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171G7008M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse PAI-1. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.
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171G7009M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse resistin. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Diabetes Standards(171I70001), which are not included.

Documents

Documents

Number Description Options
10000092626
Instruction Manual, Bio-Plex Pro Mouse Diabetes Assays
10000090940
Quick Guide, Bio-Plex Pro Mouse Diabetes Assays
5405
Bio-Plex® Multiplex System Brochure, Ver E
[ Add to Cart (Free) ]
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
5986
Mouse Diabetes Multiplex Metabolic Biomarkers Assay, Rev A
10010747
Instruction Manual, Bio-Plex™ Pro Diabetes Assays, Rev D
6342
Bio-Plex Pro Diabetes Assay Panels Product Information Sheet
[ Add to Cart (Free) ]
10024973
Bio-Plex Pro Assays Quick Guide 2, Ver B
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems