Human Inflammation Assays

Human Inflammation Assays

Key biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can now be measured in a single multiplex kit. Available in large screening panels (24- and 37-plex), a Treg 12-plex panel

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Overview

Bio-Plex Assay Ordering Options

Bio-Plex Kits

Multiplex Assay Kits
Select complete kits for multiplex target panels

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Bio-Plex Singleplex Assays

Singleplex Assays
Select individual target analytes of interest.

Select Targets

Bio-Plex Pro Human Inflammation Assays enable you to detect and quantify 37 key biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs in a single assay.

Inflammation is the hallmark of allergy, infectious diseases, and wound healing. It is a leading cause of mortality worldwide and is indicated in eight major disease areas including autoimmune diseases, cancer, cardiovascular diseases, diabetic complications, infectious diseases, metabolic disorder complications, neurological diseases, and pulmonary diseases.

Human Inflammation Assays are available in two multiplexed assay panels, as well as singleplex and custom-configured assays.

Bio-Plex Pro Human Inflammation Assays include the following analytes:

  • APRIL / TNFSF13
  • BAFF / TNFSF13B
  • sCD30 / TNFRSF8
  • sCD163
  • Chitinase-3-like 1
  • gp130 / sIL-6Rβ
  • IFN-α2
  • IFN-β
  • IFN-γ
  • IL-2
  • sIL-6Rα
  • IL-8
  • IL-10
  • IL-11
  • IL-12 (p40)
  • IL-12 (p70)
  • IL-19
  • IL-20
  • IL-22
  • IL-26
  • IL-27 (p28)
  • IL-28A / IFN-λ2
  • IL-29 / IFN-λ1
  • IL-32
  • IL-34
  • IL-35
  • LIGHT / TNFSF14
  • MMP-1
  • MMP-2
  • MMP-3
  • Osteocalcin
  • Osteopontin
  • Pentraxin-3
  • sTNF-R1
  • sTNF-R2
  • TSLP
  • TWEAK / TNFSF12

Benefits of Bio-Plex Pro Human Inflammation Assays:

  • Unique and relevant mixture of targets — the only Luminex bead-based assay kit available that combines TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs in one assay
  • Flexible ordering options — two large multiplex inflammation screening panels in premixed, ready-to-use kits (37-plex), a Treg 12-plex kit, singleplex assays, and any combination of assays available as custom kits in "you mix" or "we mix" options
  • Rapid results — multiplex data in just 3.5 hr through the use of magnetic beads and the fastest assay protocols
  • Single dilution factor for all targets — and a single-level control

Available Human Inflammation Assay Formats

Instrument Compatibility

Compatible with these readers and wash stations:

More Information

To learn more about h4erformance characteristics or how to set up your inflammation assays for optimal performance and sensitivity, download these documents:

Related Technologies

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(Discontinued) Bio-Plex Pro™ Human Inflammation Panel 1, 37-Plex
171AL001M

1 x 96-well, includes coupled magnetic capture beads, premixed detection antibodies, standards, quality control, detection antibody diluent HB, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions, for the detection of 37 human inflammation biomarkers

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(Discontinued) Bio-Plex Pro™ Human Treg Cytokine Panel, 12-Plex
171AL003M

1 x 96-well, includes coupled magnetic capture beads, premixed detection antibodies, standards, quality controls, detection antibody diluent HB, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions, for the detection of 12 Treg cytokines

Accessories

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(Discontinued) Bio-Plex® Handheld Magnetic Washer
171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
171-025001b_tn.jpg
(Discontinued) Bio-Plex Pro™ Flat Bottom Plates
171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
171-304502_tn.jpg
(Discontinued) Filter Plate
171304502
Pkg of 1, 96-well plate, with clear plastic lid and tray, for Bio-Plex Assays using the vacuum wash method, sealing tape not included
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(Discontinued) Bio-Plex Pro™ Wash Station
30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles

Kits & Reagents

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(Discontinued) Bio-Plex Pro Human Inflammation Panel 1, 37-Plex Standard, 96 assays
171DL0001

Pkg of 1 vial, lyophilized mixture of 37 standard analytes, enough for 96 assays; for use with Human Inflammation Assays and Reagent Kit III (171304090M)

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(Discontinued) Bio-Plex Pro Human Inflammation Panel 1, 37-Plex Standard, 50-pk
171DL0050
Pkg of 50 vials, lyophilized mixture of 37 standard analytes, enough for 50 x 96 assays; for use with Human Inflammation Assays and Reagent Kit III (171304090M)
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(Discontinued) Bio-Plex Pro Reagent Kit III with Flat Bottom Plate, 1 x 96-well
171304090M
1 x 96-well, includes detection antibody diluent HB, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape, for magnetic separation methods

Singleplexes

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bio-plex-pro-human-inflammation-panel-1-april-tnfsf13-set-171-BL001M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 APRIL / TNFSF13 Set
171BL001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human APRIL / TNFSF13. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-baff-tnfsf13b-set-171-BL002M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 BAFF/TNFSF13B Set
171BL002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human BAFF / TNFSF13B. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-scd30-tnfrSf8-set-171-BL003M-thumb.jpg
(Discontinued) Bio-Plex Pro™ Human Inflammation Panel 1 sCD30 / TNFRSF8 Set
171BL003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sCD30 / TNFRSF8, requires Reagent Kit III (171-304090M) for magnetic separation, and 1 vial of standards (171-DL0001)
bio-plex-pro-human-inflammation-panel-1-scd163-set-171-BL004M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 sCD163 Set
171BL004M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sCD163. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-chitinase-3-like-1-set171-BL005M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 Chitinase-3-Like 1 Set
171BL005M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human Chitinase-3-like 1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-gp130-sIL-6rbeta-set-171-BL006M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 gp130 / sIL-6Rβ Set
171BL006M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human gp130 / sIL-6Rβ. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-ifn-alpha2-set-171-BL007M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IFN-α2 Set
171BL007M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IFN-α2. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-ifn-beta-set-171-BL008M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IFN-β Set
171BL008M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IFN-β. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-ifn-gamma-set-171-BL009M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IFN-γ Set
171BL009M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IFN-γ. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-2 set-171-BL010M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-2 Set
171BL010M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-2. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
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(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 sIL-6Rα Set
171BL011M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sIL-6Rα. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-8-set-171-BL012M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-8 Set
171BL012M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-8. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-10-set-171-BL013M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-10 Set
171BL013M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-10. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-11-set-171-BL014M-thumb.jpg
(Discontinued) Bio-Plex Pro™ Human Inflammation Panel 1 IL-11 Set
171BL014M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-11. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-12-p40-set-171-BL015M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-12 (p40) Set
171BL015M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-12 (p40). Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-12-p70-set-171-BL016M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-12 (p70) Set
171BL016M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-12 (p70). Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-19-set-171-BL017M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-19 Set
171BL017M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-19. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-20-set-171-BL018M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-20 Set
171BL018M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-20. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-22-set-171-BL019M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-22 Set
171BL019M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-22. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-26-set-171-BL020M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-26 Set
171BL020M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-26. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-27-p28-set-171-BL021M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-27 (p28) Set
171BL021M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-27 (p28). Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-29-ifn-lambda-set-171-BL023M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 Set
171BL022M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-28A / IFN-λ2. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-28a-ifn-lambda2-set-171-BL022M.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-29 / IFN-λ1 Set
171BL023M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-29 / IFN-λ1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-32-set-171-BL024M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-32 Set
171BL024M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-32. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-34-set-171-BL025M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-34 Set
171BL025M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-34 . Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-il-35-set-171-BL026-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 IL-35 Set
171BL026M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-35. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-light-tnfsf14-set-171-BL027M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 LIGHT / TNFSF14 Set
171BL027M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human LIGHT / TNFSF14. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-mmp-1 set-171-BL028M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 MMP-1 Set
171BL028M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human MMP-1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-mmp-2-set-171-BL029M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 MMP-2 Set
171BL029M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human MMP-2 . Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-mmp-3-set-171-BL030M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 MMP-3 Set
171BL030M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human MMP-3. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-osteocalcin-set-171-BL031M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 Osteocalcin Set
171BL031M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human osteocalcin. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-osteopontin-set-171-BL032M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 Osteopontin Set
171BL032M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human osteopontin. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-pentraxin-3-set-171-BL033M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 Pentraxin-3 Set
171BL033M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human pentraxin-3. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-stnfr-1-set-171-BL034M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 sTNF-R1 Set
171BL034M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sTNF-R1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-stnfr-2-set-171-BL035M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 sTNF-R2 Set
171BL035M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sTNF-R2 . Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-tslp-set-171-BL036M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 TSLP Set
171BL036M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human TSLP. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.
bio-plex-pro-human-inflammation-panel-1-tweak-tnfsf12-set-171-BL037M-thumb.jpg
(Discontinued) Bio-Plex Pro Human Inflammation Panel 1 TWEAK / TNFSF12 Set
171BL037M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human TWEAK / TNFSF12. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Inflammation Panel 1 Standards (171DL0001), which are not included.

Documents

Number Description Options
6625
Bio-Plex Pro™ Human Inflammation Assays Product Information Sheet, Rev A
[ Add to Cart (Free) ]
6335
Bio-Plex™ Pro Multiplex Immunoassays Analyte Guide
10044281
Bio-Plex Pro Human Inflammation Panel 1 Quick Guide
10044282
Instruction Manual, Bio-Plex Pro Human Inflammation Panel 1, Rev A
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems