Bio-Plex Pro™ TGF-β Assays

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Overview

Magnetic bead–based multiplex assays for measuring TGF-β1, TGF-β2, and TGF-β3. Available in a premixed panel or as singleplex sets.

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Description

Description

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Bio-Plex Pro TGF-β assays are magnetic bead–based multiplex assays designed to measure levels of TGF-β in human, mouse, and rat models in diverse matrices like serum, plasma, urine, and human milk.

Analytes detected:

  • TGF-β1
  • TGF-β2
  • TGF-β3

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices, including milk and urine
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as an all-in-one, space-saving complete kit
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target

Flexible Ordering Options

  • Bio-Plex Pro TGF-β 3-plex immunoassay — a multiplex assay that detects TGF-β1, TGF-β2, and TGF-β3
  • Bio-Plex Pro TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (171304090M) and a vial of standards (171X40001) for a complete assay

TGF-β assays are not available in 10 x 96-well formats. Reagent kits in the 10 x 96-well format cannot be used for TGF-β assays.

Instrument Compatibility

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Ordering

Ordering

items

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171W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, and sealing tape for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit
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171V4001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.
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171V4002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β2. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.
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171V4003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β3. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.

Accessories

Accessories

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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
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171304500
1.5 L, wash buffer for use with Bio-Plex Pro wash stations; not for use with phosphoprotein assays
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles

Rgnts & Stds

Rgnts & Stds

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171304090M
1 x 96-well, includes detection antibody diluent HB, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape, for magnetic separation methods
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171X40001
Pkg of 1 vial, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)
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171X40501
Pkg of 50 lot-matched vials, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 50 x 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)

Documents

Documents

Number Description Options
6127
Bio-Plex Pro TGF-β Assays for Simultaneous Profiling of TGF-β1, -β2, and -β3 in Human and Animal Models (Tech Note)
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
5405
Bio-Plex® Multiplex System Brochure, Ver E
[ Add to Cart (Free) ]
6054
Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B
[ Add to Cart (Free) ]
10024984
Instruction Manual, Bio-Plex Pro TGF-β Assays, Rev A
10024986
Instruction Manual, Bio-Plex Pro Assays Quick Guide 5, Ver B
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems