Apoptosis Assays

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Overview

This highly relevant set of assays, codeveloped with leading cancer researchers at the NCI, includes novel biomarkers involved in the commitment, onset, and induction of apoptosis by the intrinsic pathway.

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Description

Description

Bio-Plex Pro RBM Apoptosis Multiplex Assays are designed for apoptosis detection in cell or tissue lysate samples. The three analyte panels comprise a highly relevant set of intracellular proteins involved in the commitment, onset, and induction of programmed cell death by the intrinsic pathway, including Bak, Bcl-2, and caspase assays, providing an alternative or supplement to a conventional annexin V assay. 

 
 Panel 1 Analytes:
  • Bak
  • Bax
  • Lamin B
  • Smac
Panel 2 Analytes:
  • Bad
  • Bax/Bcl-2 dimer
  • Bcl-xL
  • Bim
  • Mcl-1
Panel 3 Analytes:
  • Active caspase-3
  • Bcl-xL/Bak dimer
  • Mcl-1/Bak dimer
  • Survivin

Benefits of the Bio-Plex Pro RBM Multiplex Apoptosis Assays

  • Optimized for high precision and lot-to-lot reproducibility of sample measurements
  • Magnetic beads for simplified plate processing
  • 2-level quality controls with kit lot-specific ranges
  • Assay quick guide to get you started right away

Kit Components

  • 6.5 µm magnetic capture beads
  • Detection antibodies
  • Standards
  • 2-level controls
  • Standard diluent
  • Blocking buffer
  • Lysate dilution buffer (LDB)
  • Cytosolic extraction buffer (CEB)
  • 10x assay buffer
  • 10x streptavidin-PE
  • Flat bottom plate
  • Plate seals
  • Instructions

Instrument Compatibility

Compatible with these readers and wash stations:

More Information

To learn more about performance characteristics and other cancer-related panels, refer to the documents listed below:

Ordering

Ordering

items

Use the filters below to refine results!

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171WAR1CK
1 x 96-well, includes magnetic capture beads, detection antibodies, standards, controls, standard diluent, buffers, streptavidin-PE, flat bottom plate, plate seals, for the detection of 4 apoptosis markers in cell and tissue lysates
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171WAR2CK
1 x 96-well, includes magnetic capture beads, detection antibodies, standards, controls, standard diluent, buffers, streptavidin-PE, flat bottom plate, plate seals, for the detection of 5 apoptosis markers in cell and tissue lysates
bio-plex-pro-rbm-apoptosis-panel-3-171-war3ck-thumb.jpg
171WAR3CK
1 x 96-well, includes magnetic capture beads, detection antibodies, standards, controls, standard dilluent, buffers, streptavidin-PE, flat bottom plate, plate seals, for the detection of 4 apoptosis markers in cell and tissue lysates

Accessories

Accessories

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(Discontinued) Filter Plate
171304502
Pkg of 1, 96-well plate, with clear plastic lid and tray, for Bio-Plex Assays using the vacuum wash method, sealing tape not included
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays

Documents

Documents

Number Description Options
10033631
Instruction Manual, Bio-Plex Pro™ RBM Apoptosis Assays, Rev A
10033632
Bio-Plex Pro™ RBM Apoptosis Assays, Quick Guide, Rev B
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
6474
Bio-Plex Pro RBM Apoptosis Assays, Product Information Sheet, Rev A
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
6518
Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro RBM Apoptosis Assays, Rev A
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems