Apoptosis Assays

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Bio-Plex Pro RBM Apoptosis Multiplex Assays are designed for apoptosis detection in cell or tissue lysate samples. The three analyte panels comprise a highly relevant set of intracellular proteins involved in the commitment, onset, and induction of programmed cell death by the intrinsic pathway, including Bak, Bcl-2, and caspase assays, providing an alternative or supplement to a conventional annexin V assay. 

• Panel 1 Analytes:

  • Bak
  • Bax
  • Lamin B
  • Smac

• Panel 2 Analytes:

  • Bad
  • Bax/Bcl-2 dimer
  • Bcl-xL
  • Bim
  • Mcl-1

• Panel 3 Analytes:

  • Active caspase-3
  • Bcl-xL/Bak dimer
  • Mcl-1/Bak dimer
  • Survivin

Benefits of the Bio-Plex Pro RBM Multiplex Apoptosis Assays

• Optimized for high precision and lot-to-lot reproducibility of sample measurements
• Magnetic beads for simplified plate processing
• 2-level quality controls with kit lot-specific ranges
• Assay quick guide to get you started right away

Kit Components

• • 6.5 µm magnetic capture beads
  • Detection antibodies
  • Standards
  • 2-level controls
  • Standard diluent
  • Blocking buffer
  • Lysate dilution buffer (LDB)
• • Cytosolic extraction buffer (CEB)
  • 10x assay buffer
  • 10x streptavidin-PE
  • Flat bottom plate
  • Plate seals
  • Instructions

Instrument Compatibility

Compatible with these readers and wash stations:

• Bio-Plex 200 or Luminex 200 system
• Bio-Plex 3D or Luminex FLEXMAP 3D system
• Bio-Plex MAGPIX or Luminex MAGPIX system
• Bio-Plex Pro wash stations

More Information

To learn more about performance characteristics and other cancer-related panels, refer to the documents listed below:

• Product information sheet
• Instruction manual
• Quick guide
• Cancer panels product information sheet
• Cell signaling product information sheet