Bio-Plex Pro Mouse Chemokine Assays

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Overview

These assays detect and quantify chemokines from small amounts of mouse biological samples, providing a robust tool to screen even low-level analytes in a high-throughput, multiplex assay.

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Description

Description

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The Bio-Plex Pro Mouse Chemokine Panel enables you to detect and quantify chemokines from mouse biological samples, requiring only 10 µl of precious sample.

Whether you are studying oncology, immunology, autoimmunity, neurology, infectious diseases, or engaging in biomarker discovery, the Bio-Plex Pro Mouse Chemokine Panel will provide a robust, efficient tool to screen even low-level analytes in a high-throughput, multiplex assay.

Catalog Number Mouse Chemokine Singleplex-Only Sets
12002260 TECK / CCL25 Can be run as singleplex sets or multiplexed with each other as a 2-plex panel.*
12002254 MIP-2 / CXCL2
12002247 MCP 2 Available as a singleplex set only.**
Mouse Chemokine Panel, 31-plex, Catalog #12009159  (all analytes are also available as singlplex assays)
BCA-1 / CXCL13 MIP-3α / CCL20 I-TAC / CXCL11 IFN-γ
IL-4 Eotaxin / CCL11 SCYB16 / CXCL16 MCP-3 / CCL7
MIP-1α / CCL3 IL-16 GM-CSF TNF-α
CTACK / CCL27 RANTES / CCL5 KC / CXCL1 IL-1β
IL-6 Eotaxin-2 / CCL24 SDF-1α / CXCL12 MCP-5 / CCL12
MIP-1β / CCL4 IP-10 / CXCL10 I-309 / CCL1  
ENA-78 / CXCL5 MIP-3β / CCL19 MCP-1 / CCL2  
IL-10 Fractalkine / CX3CL1 TARC / CCL17  

*Cannot be multiplexed with the Bio-Plex Pro Mouse Chemokine Panel, 31-plex.

**MCP-2 requires a different dilution factor and is therefore available only as a singleplex set.

Benefits of the Mouse Chemokine Assays

  • Gain confidence in your results with a high-performing assay
  • Detect more target analytes with optimized chemistry and broad dynamic range
  • Streamline your workflow with pre-mixed reagents and a simplified magbead-based protocol
  • Obtain data for a large number of biologically-relevant targets from a small sample volume in a single experiment.

Instrument Compatibility

Compatible with these readers and wash stations:

  • Bio-Plex 200 or Luminex 200 System
  • Bio-Plex 3D or Luminex FLEXMAP 3D System
  • Bio-Plex® MAGPIX™ or Luminex MAGPIX System
  • Bio-Plex Pro Wash Station

More Information

To learn more about performance characteristics or how to set up your mouse chemokine assays for optimal performance and sensitivity, see our supporting documents:

Other Available Mouse Multiplex Assays

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Magnetic bead-based assays detect and measure levels of 43 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth, to infections, to Parkinson's disease.

Bio-Plex Pro Mouse Th17 Assays
Magnetic bead-based immunoassays detect and measure the levels of 17 Th17 cell-associated biomarkers in diverse matrices such as serum, plasma, and tissue culture supernatants. IL-17 producing T helper Cells (Th17 cells) are a new subset of T helper cells recently found to be associated with autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, and lupus. The markers in these assays were selected for their direct relevance to the mouse Th17 pathway.

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12002231-bio-plex-pro-mouse-chemokine-panel-33-plex-1x96-thumb.jpg
12009159
1 x 96-well, includes coupled magnetic capture beads, premixed detection antibodies, standards, quality control, mouse HP detection antibody diluent, standard diluent, sample diluent, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions, for the detection of 31 mouse chemokine biomarkers

Accessories

Accessories

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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles
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2239395
Pkg of 10 racks, 96 micro test tubes per rack (960 tubes), 1 ml, 8.8 x 45 mm, presterilized, not autoclavable.
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(Discontinued) Filter Plate
171304502
Pkg of 1, 96-well plate, with clear plastic lid and tray, for Bio-Plex Assays using the vacuum wash method, sealing tape not included

Rgnts & Stds

Rgnts & Stds

12002798-bio-plex-pro-reagent-kit-v-1x96-well-thumb.jpg
12002798
1 x 96-well, includes mouse HP detection antibody diluent, standard diluent, sample diluent, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape for use with magnetic separation methods
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12002796
Pkg of 1 vial, lyophilized mixture of 34 standard analytes for use with Mouse Chemokine Assays and Reagent Kit V (12002798), enough for 96 assays

Singleplexes

Singleplexes

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12002232
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse BCA-1 / CXCL13. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002233
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse CTACK / CCL27. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002234
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse ENA-78 / CXCL5. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002235
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse Eotaxin / CCL11. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002236
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse Eotaxin-2 / CCL24. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002237
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse Fractalkine / CX3CL1. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002238
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse GM-CSF. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002239
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse I-309 / CCL1. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002438
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IFN-γ. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002240
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-1β. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002439
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-2. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002440
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-4. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002241
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-6. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002242
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-10. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002243
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-16. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002244
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IP-10 / CXCL10. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002245
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse I-TAC / CXCL11. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002246
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse KC / CXCL1. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002441
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MCP-1 / CCL2. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
12002247-bio-plex-pro-mouse-chemokine-panel-mpc-2CCL8-1x96-thumb.jpg
12002247
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MCP2 / CCL8. . Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Chemokine Standards (12002796), which are not included.

Note: Bio-Plex Pro Mouse Chemokine MCP-2 /CCL8 Set can be run as a singleplex only.
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12002248
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MCP-3 / CCL7. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002249
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MCP-5 / CCL12. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002250
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MDC / CCL22. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002252
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MIP-1α / CCL3. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002253
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MIP-1β / CCL4. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002254
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MIP-2 / CXCL2. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796).

Note: Bio-Plex Pro Mouse Chemokine MIP-2 / CXCL2 Set cannot be multiplexed with the Bio-Plex Pro Mouse Chemokine Panel, 31-plex.

Bio-Plex Pro Mouse Chemokine MIP-2 / CXCL2 Set can be multiplexed with Bio-Plex Pro Mouse Chemokine TECK / CCL25 Set as a 2-plex panel.
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12002443
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MIP-3α / CCL20. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002255
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse MIP-3β / CCL19. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002256
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse RANTES / CCL5. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002257
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse SCYB16 / CXCL16. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002258
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse SDF-1α / CXCL12. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002259
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse TARC / CCL17. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)
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12002260
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse TECK / CCL25. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796).

Note: Bio-Plex Pro Mouse Chemokine TECK / CCL25 Set cannot be multiplexed with the Bio-Plex Pro Mouse Chemokine Panel, 31-plex.

Bio-Plex Pro Mouse Chemokine TECK / CCL25 Set can be multiplexed with Bio-Plex Pro™ Mouse Chemokine MIP-2 / CXCL2 Set as a 2-plex panel.
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12002444
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse TNF-α. For use with Bio-Plex Pro Reagent Kit V (12002798) and Bio-Plex Pro Mouse Chemokine Standards (12002796)

Documents

Documents

Number Description Options
10000061997
Bio-Plex Pro™ Mouse Chemokine Assay Quick Guide
10000057971
Instruction Manual, Bio-Plex Pro™ Mouse Chemokine Assays, Ver A
7050
Bio-Plex Pro Mouse Chemokine Assays Product Information Sheet
[ Add to Cart (Free) ]
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems