S3e Cell Sorter

Image
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Overview

The S3e Cell Sorter is enhanced with the AutoGimbal System, which enables hands-free motorized nozzle tip alignment. Real-time automation allows walk-away cell sorting.
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Description

Description

The S3e Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier and accessible to both novices and experts.

This compact system is equipped with either one to three lasers and up to four fluorescence detectors plus forward- and side-scatter detectors.

Features and Benefits of the S3e Cell Sorter

  • Hands-free nozzle tip alignment — features the fully automatic AutoGimbal System, driven completely by software
  • Simplified instrument setup — fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 1–4 color sorting with minimal training
  • Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system
  • 2-way cell sorting — sort two different defined populations at the same time
  • High speed and high purity — sort cells fast while maintaining high sensitivity and purity
  • Intuitive interface — user-friendly ProSort™ Software enables effortless instrument control and sort logic definition

Among the many innovations of the S3e Cell Sorter, two key state-of-the-art advancements are ProDrop Technology and the AutoGimbal System:

ProDrop Technology produces extremely accurate and precise results by:

  • Automating drop delay calculation and droplet break-off monitoring
  • Allowing drop delay calculation through direct counting of bead events
  • Simplifying one of the most complex and error-prone aspects of cell sorting

The highly sophisticated AutoGimbal System automates alignment of the nozzle tip and stream to optics by:

  • Providing hands-free alignment at all points on the stream and consistency in stream position from day to day and user to user
  • Combining picomotor fine motion positioning, imaging, and response to software algorithms using camera images

The affordable S3e Cell Sorter provides exceptional sort purity, recovery, and yield without compromising performance and sensitivity.

The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry, or for cells labeled with fluorescent markers such as antibodies labeled with PE, PE tandems, and other fluorophores excited by the 405, 488, 561, and 640 nm lasers.

Specifications

Specifications

Droplet frequency
37–43 kHz
Sorting type
True jet-in-air for high-performance sorting
Sorting rate
No hardware limitations for sort rate, limited only by droplet frequency and application
Sorting purity
>99% pure
Nozzle size
100 µm
Sorting direction
2-way sorting
Sorting collection
Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Lasers
Single-laser: 488 nm 100 mW
Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
Dual-laser: 488 and 561 or 640 nm 100 mW
Tri-laser: 405, 488, and 561 or 405, 488, and 640 or 488, 561, and 640 mW 100 mW
Detection
Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
Sensitivity
<125 MESF for FITC and PE
Temperature range
Sample and collection temperature control system: 4–37°C
Peltier solid state system
Fluidics
Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format
FCS 3.1
Dimensions
70 x 65 x 65 cm (2.3 x 2.1 x 2.1 ft)

Ordering

Ordering

items

Use the filters below to refine results!

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12007058
S3e Cell Sorter, 405, 488, and 561 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software
s3e-cell-sorter-12007059-th.jpg
12007059
S3e Cell Sorter, 405, 488, and 640 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software
s3e-cell-sorter-488-nm-145-1005-thumb-s3e-cell-sorter.jpg
1451005
S3e Cell Sorter, 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software
s3e-cell-sorter-488-561-nm-145-1006-thumb-s3e-cell-sorter.jpg
1451006
S3e Cell Sorter, 488 and 561 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software
s3e-cell-sorter-12007052-th.jpg
12007052
S3e Cell Sorter, 488, 561, and 640 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software
s3e-cell-sorter-488-nm-145-1005-thumb-s3e-cell-sorter.jpg
1451008
S3e Cell Sorter, 488 and 640 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software
s3-cell-sorter-145-1078-thumb.jpg
1451078
Biosafety System Class I for S3™ and S3e Cell Sorters, includes HEPA filter, fan blower, base, aluminum frame, 30 mil vinyl panels with magnetic attachment
s3-cell-sorter-17003806-thumb.jpg
17003806
S3e Cell Sorter, 405, 488, and 561 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I
s3-cell-sorter-17003807-thumb.jpg
17003807
S3e Cell Sorter, 405, 488, and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I
s3-cell-sorter-17003808-thumb.jpg
17003808
S3e Cell Sorter, 488, 561, and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I
s3e-cell-sorter-488-nm-with-s3-biosafety-system-class-I-145-1029-thumb-s3e-cell-sorter.jpg
1451029
S3e Cell Sorter, 488 nm 100 mW laser, 2 fluorescence detectors, with S3e Biosafety System Class I
s3e-cell-sorter-488-561-nm-with-s3-biosafety-system-class-I-145-1030-thumb-s3e-cell-sorter.jpg
1451030
S3e Cell Sorter, 488 nm and 561 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I
s3e-cell-sorter-488-561-nm-with-s3-biosafety-system-class-I-145-1030-thumb-s3e-cell-sorter.jpg
1451032
S3e Cell Sorter, 488 nm and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

Accessories

Accessories

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1441001
Pkg of 1, white sheath bottle cap assembly with quick disconnect and inline filter, for use with the S3e Cell Sorter
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1441002

Pkg of 1, blue DI water bottle cap assembly with quick disconnect and inline filter, for use with any S3™ or S3e Cell Sorter

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1441003
Pkg of 1, red waste bottle cap assembly with quick disconnect, for use with the S3e Cell Sorter
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1441005

Pkg of 1, empty filter block A with 2 empty dichroic filter holders and 3 retaining rings, for use with any S3 or S3e Cell Sorter

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1441006

Pkg of 1, empty filter block B with 2 empty dichroic filter holders and 2 retaining rings, for use with any S3 or S3e Cell Sorter

s3-individual-filter-holder-144-1007-thumb.jpg
1441007

Pkg of 1, empty filter holder with retaining ring, for use with any S3 or S3e Cell Sorter

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1441008

Pkg of 1, neutral density filter 1.0, for use with any S3™ or S3e™ Cell Sorter

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1441009

Pkg of 1, nozzle tip, 100 µm, for use with any S3 or S3e Cell Sorter

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1441010

Pkg of 1, includes 2 nozzle O-rings and 2 alignment disks, for use with any S3 or S3e Cell Sorter

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1441011
Pkg of 1, collection adaptor set, includes 1 right and 1 left holder to accommodate microscope slides and flat bottom 8-tube strips, for use with any S3™ or S3e Cell Sorter
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12005068
Pkg of 1, collection adaptor set, includes 1 right and 1 left holder to accommodate low profile 8-tube PCR strips, for use with any S3™ or S3e Cell Sorter
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(Discontinued) 2 mm Hex Driver
1441012

Pkg of 1, 2 mm hex driver, for use with any S3™ or S3e™ Cell Sorter

s3-individual-filter-holder-144-1013-thumb.jpg
1441013

Pkg of 1, empty dichroic filter holder with retaining ring, for use with any S3 or S3e Cell Sorter

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(Discontinued) S3e™ Accessory Kit
1451065

Accessory kit, includes 100 µm nozzle tip, neutral density filter 1.0, spanner wrench, 2 mm hex driver, 1 ml syringe, O-ring, and alignment disk, for use with any S3 or S3e Cell Sorter

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1451076
220–240 V, power conditioner for uninterruptible power supply, protects against power surges and outages, for use with any S3™ or S3e Cell Sorter
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1451077
100–120 V, power conditioner for uninterruptible power supply, protects against power surges and outages, for use with any S3™ or S3e Cell Sorter

Consumables

Consumables

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12012576
Pkg of 100, sterile, cup fitting, and disposable cell filters, 30 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.
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12012574
Pkg of 100, sterile, cup fitting, and disposable cell filters.Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.
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12012573
Pkg of 100, sterile, cup fitting, and disposable cell filters, 70 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.
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1451086

15 ml (3 x 5 ml), ready-to-use universal fluorescent beads for alignment verification and drop delay determination, for use on any S3™ or S3e™ Cell Sorter

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1451085
5 ml (1 x 5 ml), include 3.0–3.4 µm beads dyed with 8 different fluorescent intensities for testing sensitivity and linearity on any S3™ or S3e™ Cell Sorter
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1451081

Discontinued Product
This item has been replaced by ProLine Universal Calibration Beads (1451086)

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1451082

This product has been replaced by catalog (12012932).
There is no difference in unit volume or composition between the discontinued product and the replacement product.

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12012932

20 L (5 x 4 L), ready-to-use, sterile endotoxin- and preservative-free sheath fluid generates 160 L of 1x solution, for use on any S3 or S3e Cell Sorter

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1451083

20 L (5 x 4 L), ready-to-use, sterile endotoxin-free water in gamma-irradiated S3e Fluidic Containers, for use on any S3 or S3e Cell Sorter

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1451084

3 x 4 L, gamma-irradiated containers, for use on any S3 or S3e Cell Sorter

Download

Download

ProSort™ Software, Version 1.6, PC (Windows 7 Pro and 10)
Version-16-PC-Windows-7-Pro-and-10

S3e Cell Sorter instrument software. Version 1.6, for PC only (Windows 7 Pro and 10).
Installation instructions for existing users:

  • Users on V1.5 can go directly to V1.6 installation
  • Users on V1.4 will download and install V1.5 first (from web), before V1.6 upgrade
  • For all other ProSort versions, please contact Bio-Rad technical support
ProSort™ Software, Version 1.5, PC (Windows 7 Pro and 10)
Version-15-PC-Windows-7-Pro-and-10
S3e Cell Sorter instrument software. Version 1.5, for PC only (Windows 7 Pro and 10).

Documents

Documents

Number Description Options
7187
High-Speed Sorting of Rare Cell Subsets with High Purity and Viability Using the S3e Cell Sorter
7074
S3e™ Cell Sorter Flier
[ Add to Cart (Free) ]
6614
S3e™ Cell Sorter Brochure
[ Add to Cart (Free) ]
7261
Top 50 Journal Publications for the S3e Cell Sorter
[ Add to Cart (Free) ]
10032007
S3e™ Cell Sorter Quick Guide
10031105
Instruction Manual, S3™ and S3e™ Cell Sorters
7064
S3e Cell Sorter Laser Configuration Guide
6404
S3e™ Biosafety System Class I Flier
6503
S3e™ Biosafety System Class I Brochure
[ Add to Cart (Free) ]
10030825
ProFlow™ Sort Grade 8x Sheath Fluid Product Insert
10032248
ProFlow™ Sort Grade Water Product Insert
10033040
ProLine™ Rainbow Beads Product Insert
10041517
ProLine™ Universal Calibration Beads Product Insert
10033232
Instruction Manual, S3™ Biosafety System Class I
10041735
S3e™ Filter Block (A or B) with Dichroic Filter Holders Product Insert
10041736
S3e™ Individual Filter Holder Product Insert
7055
Identifying Heterogeneity within Rare Cell Populations by Pairing Single-Cell RNA-Seq with Cell Sorting
6806
Uncovering Actionable Correlations in Heterogeneous Cell Populations for Drug Discovery and Development
6688
Sorting Bone Marrow Mesenchymal Stem Cells with the S3™ Cell Sorter Application Note
10041737
S3e™ Individual Dichroic Filter Holder Product Insert
6609
Isolation of Stably Transfected Fluorescent Cells with the S3™ Cell Sorter Application Note
6853
Reliable Single-Cell Sorting with Bio-Rad's S3e™ Cell Sorter Application Note
6681
Discriminating Copy Number Variation from Heterogeneous Samples Using the S3™ Cell Sorter and the QX200™ Droplet Digital™ PCR System Application Note
7058
S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488 nm laser)
7059
S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488/561 nm laser)
7060
S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488/640 nm laser)
7061
S3e Cell Sorter Fluorophore and Dye Compatibility Chart (405/488/561 nm laser)
7062
S3e Cell Sorter Fluorophore and Dye Compatibility Chart (405/488/640 nm laser)
7063
S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488/561/640 nm laser)
6705
S3e™ Cell Sorter Daily Activities Guide
6777
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR
N6708
5 Tips to Secure Funding for a Flow Cytometer or Cell Sorter
10000121934
Product Information sheet, ProFlow Cell Filters for Flow Cytometry