S3e Cell Sorter
The S3e Cell Sorter is enhanced with the AutoGimbal System, which enables hands-free motorized nozzle tip alignment. Real-time automation allows walk-away cell sorting.
Overview
Discontinued Product
The S3e Cell Sorter instrument is now discontinued. However. we will continue to provide consumables, services and support for this extremely popular product. Please see details of accessories, consumables and downloads and documentation below.
The S3e Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier and accessible to both novices and experts.
This compact system is equipped with either one to three lasers and up to four fluorescence detectors plus forward- and side-scatter detectors.
Features and Benefits of the S3e Cell Sorter
- Hands-free nozzle tip alignment — features the fully automatic AutoGimbal System, driven completely by software
- Simplified instrument setup — fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 1–4 color sorting with minimal training
- Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system
- 2-way cell sorting — sort two different defined populations at the same time
- High speed and high purity — sort cells fast while maintaining high sensitivity and purity
- Intuitive interface — user-friendly ProSort™ Software enables effortless instrument control and sort logic definition
Among the many innovations of the S3e Cell Sorter, two key state-of-the-art advancements are ProDrop Technology and the AutoGimbal System:
ProDrop Technology produces extremely accurate and precise results by:
- Automating drop delay calculation and droplet break-off monitoring
- Allowing drop delay calculation through direct counting of bead events
- Simplifying one of the most complex and error-prone aspects of cell sorting
The highly sophisticated AutoGimbal System automates alignment of the nozzle tip and stream to optics by:
- Providing hands-free alignment at all points on the stream and consistency in stream position from day to day and user to user
- Combining picomotor fine motion positioning, imaging, and response to software algorithms using camera images
The affordable S3e Cell Sorter provides exceptional sort purity, recovery, and yield without compromising performance and sensitivity.
The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry, or for cells labeled with fluorescent markers such as antibodies labeled with PE, PE tandems, and other fluorophores excited by the 405, 488, 561, and 640 nm lasers.
Related Technologies
Related Products
Specifications
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
Dual-laser: 488 and 561 or 640 nm 100 mW
Tri-laser: 405, 488, and 561 or 405, 488, and 640 or 488, 561, and 640 mW 100 mW
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
Peltier solid state system
Ordering
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S3e Cell Sorter, 488 nm 100 mW laser, 2 fluorescence detectors, with S3e Biosafety System Class I
Accessories
Pkg of 1, blue DI water bottle cap assembly with quick disconnect and inline filter, for use with any S3™ or S3e Cell Sorter
Pkg of 1, empty filter block A with 2 empty dichroic filter holders and 3 retaining rings, for use with any S3 or S3e Cell Sorter
Pkg of 1, empty filter block B with 2 empty dichroic filter holders and 2 retaining rings, for use with any S3 or S3e Cell Sorter
Pkg of 1, empty filter holder with retaining ring, for use with any S3 or S3e Cell Sorter
Pkg of 1, neutral density filter 1.0, for use with any S3™ or S3e™ Cell Sorter
Pkg of 1, nozzle tip, 100 µm, for use with any S3 or S3e Cell Sorter
Pkg of 1, includes 2 nozzle O-rings and 2 alignment disks, for use with any S3 or S3e Cell Sorter
Pkg of 1, 2 mm hex driver, for use with any S3™ or S3e™ Cell Sorter
Pkg of 1, empty dichroic filter holder with retaining ring, for use with any S3 or S3e Cell Sorter
Accessory kit, includes 100 µm nozzle tip, neutral density filter 1.0, spanner wrench, 2 mm hex driver, 1 ml syringe, O-ring, and alignment disk, for use with any S3 or S3e Cell Sorter
Consumables
15 ml (3 x 5 ml), ready-to-use universal fluorescent beads for alignment verification and drop delay determination, for use on any S3™ or S3e™ Cell Sorter
5 ml (1 x 5 ml), include 3.0–3.4 µm beads dyed with 8 different fluorescent intensities for testing sensitivity and linearity on any S3™ or S3e™ Cell Sorter
20 L (5 x 4 L), ready-to-use, sterile endotoxin- and preservative-free sheath fluid generates 160 L of 1x solution, for use on any S3 or S3e Cell Sorter
20 L (5 x 4 L), ready-to-use, sterile endotoxin-free water in gamma-irradiated S3e Fluidic Containers, for use on any S3 or S3e Cell Sorter
3 x 4 L, gamma-irradiated containers, for use on any S3 or S3e Cell Sorter
Downloads
S3e Cell Sorter instrument software. Version 1.6, for PC only (Windows 7 Pro and 10).
Installation instructions for existing users:
- Users on V1.5 can go directly to V1.6 installation
- Users on V1.4 will download and install V1.5 first (from web), before V1.6 upgrade
- For all other ProSort versions, please contact Bio-Rad technical support