ZOE Fluorescent Cell Imager

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Overview

The ZOE Cell Imager simplifies fluorescence microscopy with an intuitive touch-screen interface to control the brightfield, three fluorescence channels, and integrated digital camera.
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Description

Description

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The ZOE Fluorescent Cell Imager eliminates the complexities of cell imaging associated with traditional microscopes. This fluorescence imaging system combines the ease of use of a personal tablet with the power of an inverted microscope.

An Android-based platform, the ZOE Cell Imager uses an intuitive touch-screen interface to control the brightfield, three fluorescence channels, and the integrated digital camera.

The ZOE Fluorescent Cell Imager is a complete digital imaging system, allowing users to view samples, capture and store images, and create multicolor overlays. Thanks to the built-in light shield, the ZOE Cell Imager does not require a darkroom for fluorescence imaging.

Features and Benefits of the ZOE Fluorescent Cell Imager

  • Simplified cell imaging — the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training
  • Flexible operation — brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications
  • Fluorescence imaging at your bench — light shield permits fluorescence imaging in ambient light
  • Robust construction — fully integrated system with long-life LEDs, ready for intensive daily use
  • LED light sources — thousands of hours of illumination that are instantly ready after power-on
  • Large viewing area — the motorized stage and wide field of view allow you to see more of your sample, faster
  • Small footprint — compact size accommodates crowded lab benches

Applications of the ZOE Fluorescent Cell Imager

Use the ZOE Cell Imager to check/screen samples prior to high-content analysis (HCA), high-throughput screening (HCS), confocal imaging, or fluorescence-activated cell sorting (FACS). With a brightfield and three fluorescent channels, the ZOE Cell Imager has all the features needed for daily cell culture work as well as fluorescent applications:

  • Visual estimation of cell confluency
  • Observation of general cell health and morphology
  • Cell growth and proliferation monitoring
  • Capturing cell images (with or without fluorescent labels)
  • Visualization of expressed fluorescent proteins
  • Immunofluorescent protein localization
  • Estimation of transfection efficiency

Illumination Light Sources

  • Blue channel uses a UV LED
  • Green channel uses a blue LED
  • Red channel uses a green LED
  • Brightfield channel uses a ring of multiple green LEDs for reduced chromatic aberration

Fluorophores Compatible with the ZOE Fluorescent Cell Imaging System

This is not a comprehensive list; other dyes and fluorescent proteins with compatible excitation and emission spectra can also be used.

Blue Channel
Excitation: 355/40 nm
Emission: 433/36 nm
Green Channel
Excitation: 480/17 nm
Emission: 517/23 nm
Red Channel
Excitation: 556/20 nm
Emission: 615/61 nm
PureBlu DAPI Nuclear
Staining Dye
CytoTrack Green
511/525
ReadiLink 555/570
Antibody Labeling Kit
PureBlu Hoechst 33342
Nuclear Staining Dye
ReadiLink 492/516
Antibody Labeling Kit
ReadiLink 594/610
Antibody Labeling Kit
ReadiLink 350/440
Antibody Labeling Kit
VivaFix 498/521
Cell Viability Assay*
VivaFix 547/573
Cell Viability Assay
VivaFix 353/442
Cell Viability Assay
CFDA-SE VivaFix 583/603
Cell Viability Assay
Alexa Fluor 350 Acridine Orange Alexa Fluor 546
Alexa Fluor 405 Alexa Fluor 488 dye* Alexa Fluor 568
Cascade Blue BODIPY Fl* Alexa Fluor 594
CellTracker Blue Calcein AM Alexa Fluor 610
DAPI* DiO Cy3*
Hoechst* EGFP* Dil Stain
LysoTracker Blue ER-Tracker Green DsRed*
Marina Blue FITC* ER-Tracker Red
NucBlue Fixed MitoTracker Green FM mCherry*
NucBlue Live SYTO 9, SYTO 13, SYTO 16 mStrawberry
SYTOX Green MitoTracker Red*
Tubulin Green mKate*
YFP* RFP*
SYTOX Orange
SYTO 84, SYTO 85
Texas Red*

* R&D Tested

Specifications

Specifications

Imaging channels
Brightfield channel and 3 fluorescence channels (blue, green, and red)
Light source
Blue channel: UV LED
Green channel: blue LED
Red channel: green LED
Brightfield channel: multiple green LEDs (reduces chromatic aberration)
User interface
10.1 in. color (26 cm) touch-screen LCD monitor, with anti-glare and anti-fingerprint treatment, 1,280 x 768 pixel image resolution, 80–180° angle tilt range
Focusing mechanism
Coarse and fine, manual adjustment
Camera
Monochrome camera, 12 bit CMOS, 5 megapixels
Data format
JPEG, TIFF, or RAW image files
Image merge
Images from up to 4 channels can be overlaid
Data storage
16 GB internal memory (~2,500 JPEG files,1,500 TIFF files, 400–800 RAW files)
Data export
Yes, 2 USB ports
Display output
Yes, 1 HDMI port
Objective
20x
Numerical aperture
0.40
Display magnification
Standard: 175x; zoom: 700x
Maximum imaging area
0.70 mm2 field of view
Motorized stage
6 mm travel in X, Y direction, touch-screen control of travel speed and direction
Compatible with
Flasks: T25, T75, or T225
Multiwell plates: 6-, 12-, 24-, 48-, 96-, or 384-well microplates
Dishes: 35 mm, 60 mm, or 100 mm
Slides: chamber slides or standard glass microscopy slides
Software
Stand-alone Android operating system; PC is not required for operation
Instrument size (L x W x H)
33 x 32 x 30 cm (13 x 12.6 x 11.6 in.)
Instrument weight
9 kg (19.7 lb)

Ordering

Ordering

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1450031
Stand-alone fluorescent cell imager, includes instrument, power supply, USB flash drive; 120–240 V

Kits & Reagents

Kits & Reagents

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1351001
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351002
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351003
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
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1351004
Pkg of 1, kit includes dye, reaction buffer, and quench buffer for labeling 2 x 50 µg of antibodies
vivafix-353-442-cell-viability-assay-sku-135-1111-thumb.jpg
1351111
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
vivafix-498-521-cell-viability-assay-sku-135-1115-thumb.jpg
1351115
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
vivafix-547-573-cell-viability-assay-sku-135-1116-thumb.jpg
1351116
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
vivafix-583-603-cell-viability-assay-sku-135-1117-thumb.jpg
1351117
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays
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(Discontinued) CFDA-SE
1351201
Pkg of 1, 5 x 100 µg vials
pureblu-dapi-nuclear-staining-dye-sku-135-1303-thumb.jpg
1351303
Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder
pureblu-hoechst-33342-nuclear-staining-dye-sku-135-1304-thumb.jpg
1351304
Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder

Download

Download

ZOE Fluorescent Cell Imager Software and Firmware
SOFT-ZOE-Cell-Imager-UI-FW
Software and firmware update for the ZOE Cell Imager.
Vision board driver version: 2.009. Driver board firmware version: 1.033. ZOE Cell Image Software version: 2.257

Documents

Documents

Number Description Options
6865
ZOE Fluorescent Cell Imager Publication List, Ver A
6585
ZOE™ Fluorescent Cell Imager Brochure, Rev A
[ Add to Cart (Free) ]
10041704
ZOE Fluorescent Cell Imager Instruction Manual
10041709
ZOE™ Fluorescent Cell Imager Quick Guide, Rev B
6853
Reliable Single-Cell Sorting with Bio-Rad's S3e™ Cell Sorter Application Note