ProLine Universal Calibration Beads #1451086

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Overview

15 ml (3 x 5 ml), ready-to-use universal fluorescent beads for alignment verification and drop delay determination, for use on any S3™ or S3e™ Cell Sorter

Safety Data Sheet

Safety Data Sheet

Description

Description

Use ProLine Universal Calibration Beads to specifically verify the alignment performance and to determine the drop delay on S3e Cell Sorter (488, 488/561, and 488/640 nm laser configurations).

  • Bypass the dilution step with ready-to-use fluorescent beads
  • Perform both alignment and drop delay with one reagent in less than 7 minutes
  • Achieve automated alignment verification and drop delay determination with ProSort™ Software

With the release of ProSort Software version 1.6, users are no longer required to upload an associated bead lot file prior to using a new lot of ProLine Universal Calibration Beads. For instruments running an earlier version of ProSort Software (V1.5, 1.4, etc.), download the UNIVERSAL bead lot file in order to use a bead lot not found on the list below.

To download and import a new bead lot file, follow the instructions below.

1. Double click on your bead lot from the table below.
Bead Lot File
UNIVERSAL
BR092017C
BR091417B
BR082117A
BR082515C
BR082715B
BR021617B
BR100616A
BR041416AB
BR042915B
BR031215B
BR120612A
BR031215
101812A
2. Save the file to the S3 or S3e desktop or on a thumb drive.
3. Double click on the ProSort Software icon.
4. Log in to ProSort Software as an administrator user.
5. Select Administration tab > Edit QC Criteria.
6. Click to import the new bead lot file. Select the new bead lot file and click open.

 

The new bead lot is now saved in ProSort Software and added to the bead lot dropdown menu for future use.

 

Supporting Documentation

For step-by-step instructions to ensure that ProLine Universal Calibration Beads are prepared for optimal use, refer to the ProLine™ Universal Calibration Beads Product Insert.

Additional S3e Cell Sorter Consumables

    • ProFlow™ Sort Grade 8x Sheath Fluid, preservative-free (1451082)
      Generate 160 L of 1x sterile sheath solution for use on the S3e Cell Sorter

 

    • ProFlow Sort Grade Water (1451083)
      Obtain optimal sort results with this sterile, endotoxin-free solution 

 

 

  • ProLine Rainbow Beads (1451085)
    Test the sensitivity and linearity of S3e Cell Sorter

 

Specifications

Specifications

Compatible S3 laser configuration
Compatible with all S3 or S3e laser configurations
Contents
3 x 5 ml
Concentration
1 x 106 beads/ml
Bead size
6 µm
Storage Buffer Composition
Deionized water with 0.02% sodium azide and 0.01% NP-40
Storage
Store at 2–8°C. Do not freeze.