S3e™ Cell Sorter (405/488/640 nm) with S3e Biosafety System Class I #17003807

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Overview

S3e Cell Sorter, 405, 488, and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

Description

Description

Use the S3e Cell Sorter with the S3e Biosafety System Class I Containment Hood to sort cells expressing fluorescent proteins or with fluorescent markers such as labeled antibodies. This biosafety system protects both users and the environment from aerosols created during the cell sorting process.

Product Contents

This compact, affordable, integrated cell sorting system includes the following:

  • S3e Cell Sorter with 3 lasers (405/488/640 nm) and 4 detectors (12007059)
  • S3e Biosafety System Class I (1451078)

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Specifications

Specifications

S3e Cell Sorter
Droplet frequency
37–43 kHz
Sorting type
True jet-in-air for high-performance sorting
Sorting rate
No hardware limitations for sort rate, limited only by droplet frequency and application
Sorting purity
>99% pure
Nozzle size
100 µm
Sorting direction
2-way sorting
Sorting collection
Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Lasers
405, 488, and 640 nm 100 mW lasers
Detection
Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
2 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
Sensitivity
<125 MESF for FITC and PE
Temperature range
Sample and collection temperature control system: 4–37°C Peltier solid-state system
Fluidics
Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format
FCS 3.1
Dimensions (W x D x H)
70 x 65 x 65 cm (2.3 x 2.1 x 2.1 ft)
S3e Biosafety System Class I
Power requirements
100–240 VAC, 50–60 Hz, 1.25 Amp
Power and efficiency
125 W
Heat load
50 W at <85 BTU/hr
Airflow
175 ft3 per minute
Blower
R1G 175 DC motorized impeller
Fuse
2.0 A slow blow
Weight
52 lb
Dimensions (W x D x H)
135 x 96 x 146 cm (4.4 x 4.7 x 3.1 ft)
Sound level
65 dB at maximum airflow
Operating temperature
18–25°C
Operating humidity
20–60%
HEPA filtration
99.997% or better on 0.3 μm- IEST-RP-CC-034
HEPA filters
23.625 x 23.625 x 3.54 in, certify annually
Top panel
Clear polycarbonate
Base floor
High-density polyethylene
Vinyl sides
30 mil PVC film and 18 oz vinyl-coated polyester
Frame
25 mm anodized aluminum T-slot profile