CRISPR Gene Editing Workflow

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Solutions for Gene Editing Workflows

CRISPR gene editing provides versatile workflows that enable researchers to edit their desired target genes efficiently and accelerate therapeutic development. Explore our solutions for gene editing workflows.

CRISPR Gene Editing Tools

The advent of CRISPR as a gene editing tool has revolutionized drug discovery and development in a very short time. The CRISPR-Cas9 system uses short RNAs to direct the Cas9 nuclease to target specific DNA sequences for editing. Because CRISPR uses RNA rather than a protein to target nuclease activity, CRISPR-Cas9 activity can be easily and accurately retargeted by synthesizing a new guide RNA (gRNA). The gRNA effector also makes this technique amenable to high-order multiplexing, for example, large-scale screening using hundreds or thousands of gRNAs in a single experiment. Consequently, complex studies that once took years to develop in animal models can now be completed in just a few months and at a fraction of the cost.

The speed, low cost, and transferability of CRISPR-Cas9 gene editing to almost any species is redefining what is possible in R&D. More and more new applications for CRISPR are published monthly. From creating complex, multi-gene disease models and whole-genome knockout screens in less than two months to creating inducible systems and generating Cas9 variants that act as epigenetic regulators, the development of CRISPR tools has not only accelerated R&D, CRISPR-Cas9 gene editing is emerging as part of the solution for curbing the cost of drug development and reducing late-stage failure rates.

CRISPR Gene Editing Workflow

  • Transfection

    Transfection

    Identification of the best method for delivering the CRISPR-Cas9 system into the cells of interest is the first step in the gene editing workflow. When considering which transfection method to use, transfer efficiency and subsequent cell viability are important factors.

  • Enrichment_and_Single_Cell_Isolation

    Enrichment and Single-Cell Isolation

    Enrichment for cells of interest occurs after cells have been transfected. In this step, only those cells that carry the desired edits are identified and expanded. Enrichment also reduces the number of passages cells undergo, ensuring healthy cells for downstream analysis.

  • Confirmation_of_Edits

    Confirmation of Edits

    It is important to confirm that target cells have been successfully edited prior to moving on to downstream assays. This can be accomplished either through direct detection of edits using genomic methods or through indirect detection using cellular or proteomic methods.

  • Downstream_Analysis

    Downstream Analysis

    Phenotype investigation can begin once it has been confirmed that the cells are correctly edited. Further perturbation of the system with a drug as part of a cell-based assay may be desired during target or lead discovery and validation.

Companion Products for Gene Editing Workflows

  • Gene Pulser Xcell Electroporation Systems

    Gene Pulser Xcell Electroporation Systems

    • Universal electroporation — transfects all cell types: primary and stem cells, bacteria and yeast
    • Preset protocols — includes the most common mammalian and bacterial cell types
    • Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells
  • lsr_transfectin_lipid_reagent_pdtn

    TransFectin Lipid Reagent

    • High-efficiency transfections and transgene activity for a broad range of cells
    • Low cytotoxicity helps maintain cell viability
    • Flexibility — excellent performance at culture densities between 40—90% and in the presence or absence of serum-containing medium
    • Simple 3-step protocol and no post-transfection media changes required for most cell types
  • qPCR

    ZOE Fluorescent Cell Imager

    • Simplified cell imaging — the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training
    • Flexible operation — brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications
  • Ze5™ Cell Analyzer

    TC20 Automated Cell Counter

    • Accurate cell counts in one simple step
    • Auto-focus technology and sophisticated cell-counting algorithm
  • S3e Cell Sorter

    S3e Cell Sorter

    • Utilizes stream-in-air fluid dynamics for a more gentle high-speed sort
    • Features Lossless Cell Sorting mode for maximum recovery of rare-cell populations
    • Sort status email and text notifications allow the user to multitask during a sort
  • qPCR

    ZOE Fluorescent Cell Imager

    • Simplified cell imaging — the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training
    • Flexible operation — brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications
  • ddpcr-prod-nav-systems

    QX200 Droplet Digital PCR System

    • Detection of small or large edits with absolute quantification
    • Most precise and sensitive digital PCR solution for all applications
    • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
    • Flexible assay setup for high sensitivity or high throughput
  • qPCR

    CFX Real-Time PCR Detection System

    • Sensitive, reliable detection for singleplex and multiplex real-time PCR reactions
    • 2–5 color multiplexing, advanced optical technology, and precise gradient temperature control
    • CFX Maestro Software for superior data collection, visualization, and statistical analysis
  • pcr-master-mixes-kits-thumb-standard-pcr-reagents

    iProof High-Fidelity Real-Time PCR Reagents

    • High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
    • Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall run times
    • Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)
  • Real-Time_PCR Supermixes_and Kits

    Real-Time PCR Supermixes and Kits

    • Any instrument — universal reference dye is compatible with all qPCR platforms
    • Any chemistry — supermixes for SYBR Green or probe-based detection chemistry
    • Any conditions — our patented Sso7d fusion polymerase guarantees superior qPCR performance under varying and/or challenging conditions
  • V3 Western Workflow

    V3 Western Workflow — Visualize, Verify, Validate

    A five-step streamlined western blotting protocol with innovative tools including proprietary stain-free imaging:

    • Faster run and transfer times
    • Checkpoints at every step
    • Quantitation made simple
  • PrecisionAb Validated_Western_Blotting_Antibodies

    PrecisionAb Validated Western Blotting Antibodies

    • Robust validation — performed on up to 12 different cell lysates with endogenous protein levels
    • Reproducible data — stringent batch-to-batch QC
    • High specificity and sensitivity — low nonspecific binding and strong signal from target proteins
  • qPCR

    ZOE Fluorescent Cell Imager

    • Simplified cell imaging — the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training
    • Flexible operation — brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications
  • CFX Automation System II

    CFX Automation System II

    • Works with all CFX Real-Time PCR Systems to facilitate automated workflows, large-volume data generation, and rapid data analysis
    • Saves valuable lab space — run two CFX Systems with a single plate handler in a compact footprint
    • Run up to up to 84 plates per day
  • Digital PCR Systems

    QX200 Droplet Digital PCR System

    • Detection of small or large edits with absolute quantification
    • Most precise and sensitive digital PCR solution for all applications
    • Digital PCR chemistry optimized for TaqMan hydrolysis probes or EvaGreen assays
    • Flexible assay setup for high sensitivity or high throughput
  • ddPCR  Genome Edit Detection Assays

    ddPCR Genome Edit Detection Assays

    • Enable rapid assessment of HDR and NHEJ edits generated by CRISPR-Cas9 system or other targeted genome editing tools
    • Detect editing events present at frequencies ≤ 0.5%
    • Provide absolute quantification of genome editing events from as little as 5 ng of total gDNA (cells in a single well of a 96-well plate)
  • Ze5™ Cell Analyzer

    ZE5 Cell Analyzer

    • Analysis of up to 30 parameters from a single sample, expanding the number of biomarkers that can be monitored in a cell-based assay
    • Measures rare or transient cell populations quickly
  • V3 Western Workflow

    V3 Western Workflow — Visualize, Verify, Validate

    A five-step streamlined western blotting protocol with innovative tools including proprietary stain-free imaging:

    • Faster run and transfer times
    • Checkpoints at every step
    • Quantitation made simple
  • BPMI

    Bio-Plex Multiplex Immunoassays

    • Measure up to 500 unique analytes in a single sample
    • Assay and panels for over 450 biologically relevant targets: inflammation, disease, cancer, cell signaling and growth, apoptosis, toxicity, and more
    • Choose premade or custom configurations, or develop your own assays for new targets
  • Bio-Plex 3D Suspension Array System

    Bio-Plex Readers and Tools

    • Premium instruments, software, calibration, and validation kits to ensure consistent, reproducible results
    • Use our Bio-Plex Multiplex Immunoassays, powered by Luminex xMAP Technology
    • Measure up to 500 unique analytes in a single sample
PV47ZCRA83CC [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [3.230.154.129, 10.232.18.216] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/applications-technologies/crispr-gene-editing-workflow] [x-amzn-trace-id] = [Root=1-5edc52b4-b3217bbed9de8af19080aed1] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=PV47ZCRA83CC&WT.mc_id=191204027431] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/applications-technologies/crispr-gene-editing-workflow] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] 191204027431 AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/crispr-gene-editing-workflow LSR PV47ZCRA83CC CRISPR Gene Editing Workflow CRISPR Gene Editing Workflow Le Gao CRISPR Gene Editing Workflow CRISPR Gene Editing Workflow CRISPR, Gene, Editing, Workflow, 08/22/19 05:13 AM 08/21/29 07:14 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Applications/Targeted_Genome_Editing N 0 <div class="pagenav"><img src="/webroot/web/images/opt/prodnav-arrow-top.png" alt="" /> <table border="0"> <tbody> <tr> <td class="pagenavhead"> <h3>On This Page</h3> </td> <td class="pagenavfirst"><a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Page Nav&amp;tgp_pcp_link=Personalized Therapies for Cancer','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="#module1">Solutions for Gene Editing Workflows</a></td> <td><a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Page Nav&amp;tgp_pcp_link=4 Main Steps of IO Application/Process','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="#module2">CRISPR Gene Editing Workflow</a></td> <td><a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Page Nav&amp;tgp_pcp_link=4 Main Steps of IO Application/Process','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="#module3">Companion Products for Gene Editing Workflows </a></td> <td class="pagenavlast"><a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Page Nav&amp;tgp_pcp_link=Promising Immunotherapies','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="#module4">Resources</a></td> </tr> </tbody> </table> </div> <div class="opt-module opt-module-first opt-module-noborder"> <div class="corner-box corner-box-promo marginbttm-15"><img src="/webroot/web/images/general/global/english/have-questions-icon.png" border="0" alt="Consult with an Specialist" style="width: 50px; height: auto;" /> <h2>Need help with CRISPR?</h2> <a href="/featured/forms/en/TWAS-LSR-02-2019.html" class="bttn-block no-target-style"><span class="bttn-orange">Consult with a Specialist</span></a> <div class="hidden US-only CA-only"> <div class="dotteddivider"></div> <img src="/webroot/web/images/lse/support/CRISPR_KIT-callout_img_160x160.png" border="0" alt="CRISPR KIT" style="width: 75px; height: auto;" width="160" height="160" caption="false" /> <h2>Teaching CRISPR?</h2> <p>See <a href="/en-us/product/out-blue-crispr-kit-genotyping-extension?ID=Q0JGD4E08O1Y&amp;WT.mc_id=191204027431">Out of the Blue CRISPR Kit and Genotyping Extension</a>.</p> </div> </div> <div><a name="module1"></a></div> <h2 class="nextp">Solutions for Gene Editing Workflows</h2> <p>CRISPR gene editing provides versatile workflows that enable researchers to edit their desired target genes efficiently and accelerate therapeutic development. <a href="#module3">Explore our solutions for gene editing workflows.</a></p> <h3>CRISPR Gene Editing Tools</h3> <p>The advent of CRISPR as a gene editing tool has revolutionized drug discovery and development in a very short time. The CRISPR-Cas9 system uses short RNAs to direct the Cas9 nuclease to target specific DNA sequences for editing. Because CRISPR uses RNA rather than a protein to target nuclease activity, CRISPR-Cas9 activity can be easily and accurately retargeted by synthesizing a new guide RNA (gRNA). The gRNA effector also makes this technique amenable to high-order multiplexing, for example, large-scale screening using hundreds or thousands of gRNAs in a single experiment. Consequently, complex studies that once took years to develop in animal models can now be completed in just a few months and at a fraction of the cost.</p> <p>The speed, low cost, and transferability of CRISPR-Cas9 gene editing to almost any species is redefining what is possible in R&amp;D. More and more new applications for CRISPR are published monthly. From creating complex, multi-gene disease models and whole-genome knockout screens in less than two months to creating inducible systems and generating Cas9 variants that act as epigenetic regulators, the development of CRISPR tools has not only accelerated R&amp;D, CRISPR-Cas9 gene editing is emerging as part of the solution for curbing the cost of drug development and reducing late-stage failure rates.</p> </div> <!--<div><img class="opt-horizdivider" src="/webroot/web/images/opt/digital-pcr/horiz-gradient-divider.jpg" alt="" /></div>--> <div><a name="module2"></a></div> <div class="opt-module opt-module-wf opt-module-wf-gewf"> <h2>CRISPR Gene Editing Workflow</h2> <ul class="list-set list-full-col list-set-th list-set-vert-wf"> <li><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-transfection?ID=PV48319V5F6X"><img src="/webroot/web/images/lsr/solutions/applications/genome_editing/io-wf-Transfection-icons.png" alt="Transfection" width="123" height="123" caption="false" /></a> <h3><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-transfection?ID=PV48319V5F6X">Transfection</a></h3> <p>Identification of the best method for delivering the CRISPR-Cas9 system into the cells of interest is the first step in the gene editing workflow. When considering which transfection method to use, transfer efficiency and subsequent cell viability are important factors.</p> </li> <li><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-enrichment-single-cell-isolation?ID=PV4845JOA571"><img src="/webroot/web/images/lsr/solutions/applications/genome_editing/io-wf-Enrichment_and_Single_Cell_Isolation-icons.png" alt="Enrichment_and_Single_Cell_Isolation" width="123" height="123" caption="false" /></a> <h3><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-enrichment-single-cell-isolation?ID=PV4845JOA571">Enrichment and Single-Cell Isolation</a></h3> <p>Enrichment for cells of interest occurs after cells have been transfected. In this step, only those cells that carry the desired edits are identified and expanded. Enrichment also reduces the number of passages cells undergo, ensuring healthy cells for downstream analysis.</p> </li> <li><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-confirmation-edits?ID=PV485RGYDN7E"><img src="/webroot/web/images/lsr/solutions/applications/genome_editing/io-wf-Confirmation_of_Edits-icons.png" alt="Confirmation_of_Edits" width="123" height="123" caption="false" /></a> <h3><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-confirmation-edits?ID=PV485RGYDN7E">Confirmation of Edits</a></h3> <p>It is important to confirm that target cells have been successfully edited prior to moving on to downstream assays. This can be accomplished either through direct detection of edits using genomic methods or through indirect detection using cellular or proteomic methods.</p> </li> <li><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-downstream-analysis?ID=PV486OMC1ORV"><img src="/webroot/web/images/lsr/solutions/applications/genome_editing/io-wf-Downstream_Analysis-icons.png" alt="Downstream_Analysis" width="123" height="123" caption="false" /></a> <h3><a href="/en-us/applications-technologies/crispr-gene-editing-workflow-downstream-analysis?ID=PV486OMC1ORV">Downstream Analysis</a></h3> <p>Phenotype investigation can begin once it has been confirmed that the cells are correctly edited. Further perturbation of the system with a drug as part of a cell-based assay may be desired during target or lead discovery and validation.</p> </li> </ul> </div> <div><a name="module3"></a></div> <div class="opt-module opt-module-tabs opt-module-noborder"> <h2 class="nextp">Companion Products for Gene Editing Workflows</h2> <div id="rotate"> <ul class="ui-tabs-nav"> <li class="ui-tabs-selected"><a href="#fragment-3"><span>Transfection</span></a></li> <li class=""><a href="#fragment-4"><span>Cell Enrichment and Isolation</span></a></li> <li class=""><a href="#fragment-5"><span>Confirmation</span></a></li> <li class=""><a href="#fragment-6"><span>Downstream Analysis</span></a></li> </ul> <div class="product-tabs"> <div class="box_middle"> <div class="set"> <div class="middle"> <div id="fragment-3" class="ui-tabs-panel"> <ul class="list-set list-full-col list-set-th"> <li><a href="/en-us/product/gene-pulser-xcell-electroporation-systems?ID=b1a35eb3-d55c-47b3-aaf3-95e4d1d85848"><img src="/webroot/web/images/lsr/products/gene_transfer_rnai/category_thumb/global/gene-pulser-xcell-electroporation-systems-thumb-electroporation-systems.jpg" alt="Gene Pulser Xcell Electroporation Systems" caption="false" width="125" height="81" /></a> <h3><a href="/en-us/product/gene-pulser-xcell-electroporation-systems?ID=b1a35eb3-d55c-47b3-aaf3-95e4d1d85848">Gene Pulser Xcell Electroporation Systems</a></h3> <ul> <li>Universal electroporation &mdash; transfects all cell types: primary and stem cells, bacteria and yeast</li> <li>Preset protocols &mdash; includes the most common mammalian and bacterial cell types</li> <li>Protocol library &mdash; collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells</li> </ul> </li> <li><a href="/en-us/product/transfectin-lipid-reagent?ID=680dace0-c2a1-451c-ba7f-d15a2294aa52"><img src="/webroot/web/images/lsr/products/gene_transfer_rnai/category_thumb/global/lsr_transfectin_lipid_reagent_pdtn.jpg" alt="lsr_transfectin_lipid_reagent_pdtn" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/product/transfectin-lipid-reagent?ID=680dace0-c2a1-451c-ba7f-d15a2294aa52">TransFectin Lipid Reagent</a></h3> <ul> <li>High-efficiency transfections and transgene activity for a broad range of cells</li> <li>Low cytotoxicity helps maintain cell viability</li> <li>Flexibility &mdash; excellent performance at culture densities between 40&mdash;90% and in the presence or absence of serum-containing medium</li> <li>Simple 3-step protocol and no post-transfection media changes required for most cell types</li> </ul> </li> <li><a href="/en-us/product/zoe-fluorescent-cell-imager?ID=N74CIZE8Z"><img src="/webroot/web/images/lsr/products/cell_imaging/product_thumb/global/cell-imaging-thumb-zoe-fluorescent-cell-imager.jpg" alt="qPCR" caption="false" width="125" height="81" /></a> <h3><a href="/en-us/product/zoe-fluorescent-cell-imager?ID=N74CIZE8Z">ZOE Fluorescent Cell Imager</a></h3> <ul> <li>Simplified cell imaging &mdash; the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training</li> <li>Flexible operation &mdash; brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications</li> </ul> </li> <li><a href="/en-us/product/tc20-automated-cell-counter?ID=M7FBG34VY"><img src="/webroot/web/images/lsr/products/cell_imaging/product_thumb/global/TC20_Automated_Cell_Counter_th.jpg" alt="Ze5&trade; Cell Analyzer" caption="false" width="125" height="81" /></a> <h3><a href="/en-us/product/tc20-automated-cell-counter?ID=M7FBG34VY">TC20 Automated Cell Counter</a></h3> <ul> <li>Accurate cell counts in one simple step</li> <li>Auto-focus technology and sophisticated cell-counting algorithm</li> </ul> </li> </ul> </div> <div id="fragment-4" class="ui-tabs-panel ui-tabs-hide"> <ul class="list-set list-full-col list-set-th"> <li><a href="/en-us/product/s3e-cell-sorter?ID=OK1GROIVK"><img src="/webroot/web/images/lsr/products/flow_cytometry/category_thumb/global/s3e-cell-sorter-thumb-flow-cytometry.jpg" alt="S3e Cell Sorter" /></a> <h3><a href="/en-us/product/s3e-cell-sorter?ID=OK1GROIVK">S3e Cell Sorter</a></h3> <ul> <li>Utilizes stream-in-air fluid dynamics for a more gentle high-speed sort</li> <li>Features Lossless Cell Sorting mode for maximum recovery of rare-cell populations</li> <li>Sort status email and text notifications allow the user to multitask during a sort</li> </ul> </li> <li><a href="/en-us/product/zoe-fluorescent-cell-imager?ID=N74CIZE8Z"><img src="/webroot/web/images/lsr/products/cell_imaging/product_thumb/global/cell-imaging-thumb-zoe-fluorescent-cell-imager.jpg" alt="qPCR" caption="false" width="125" height="81" /></a> <h3><a href="/en-us/product/zoe-fluorescent-cell-imager?ID=N74CIZE8Z">ZOE Fluorescent Cell Imager</a></h3> <ul> <li>Simplified cell imaging &mdash; the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training</li> <li>Flexible operation &mdash; brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications</li> </ul> </li> </ul> </div> <div id="fragment-5" class="ui-tabs-panel ui-tabs-hide"> <ul class="list-set list-full-col list-set-th"> <li><a href="/en-us/category/digital-pcr?ID=M9HE2R15"><img src="/webroot/web/images/opt/digital-pcr/ddpcr-prod-nav-systems.png" alt="ddpcr-prod-nav-systems" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/category/digital-pcr?ID=M9HE2R15">QX200 Droplet Digital PCR System </a></h3> <ul> <li>Detection of small or large edits with absolute quantification</li> <li>Most precise and sensitive digital PCR solution for all applications</li> <li>Flexible digital PCR chemistry &mdash; optimized for TaqMan Hydrolysis Probe and EvaGreen Assays</li> <li>Flexible assay setup for high sensitivity or high throughput</li> </ul> </li> <li><a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db"><img src="/webroot/web/images/lsr/products/amplification_pcr/category_thumb/global/cfx96-touch-real-time-pcr-detection-system-thumb-real-time-pcr-systems.jpg" alt="qPCR" /></a> <h3><a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">CFX Real-Time PCR Detection System</a></h3> <ul> <li>Sensitive, reliable detection for singleplex and multiplex real-time PCR reactions</li> <li>2&ndash;5 color multiplexing, advanced optical technology, and precise gradient temperature control</li> <li>CFX Maestro Software for superior data collection, visualization, and statistical analysis</li> </ul> </li> <li><a href="/en-us/product/high-fidelity-pcr-kits?ID=NVEO59KG4"><img src="/webroot/web/images/lsr/products/amplification_pcr/category_thumb/global/pcr-master-mixes-kits-thumb-standard-pcr-reagents.jpg" alt="pcr-master-mixes-kits-thumb-standard-pcr-reagents" width="125" height="80" caption="false" /></a> <h3><a href="/en-us/product/high-fidelity-pcr-kits?ID=NVEO59KG4">iProof High-Fidelity Real-Time PCR Reagents</a></h3> <ul> <li>High fidelity &mdash; iProof DNA polymerase is 52-fold more accurate than Taq polymerase</li> <li>Speed &mdash; high processivity dramatically reduces extension (15&ndash;30 sec/kb) and overall&nbsp;run times</li> <li>Successful amplification of long products with higher yields &mdash; fragments up to 37 kb are amplified in less time and with less enzyme (0.25&ndash;1 unit/reaction)</li> </ul> </li> <li><a href="/en-us/category/real-time-pcr-supermixes-kits?ID=M87F1J8UU"><img src="/webroot/web/images/lsr/products/gene_transfer_rnai/category_thumb/global/Real-Time_PCR Supermixes_and Kits.png" alt="Real-Time_PCR Supermixes_and Kits" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/category/real-time-pcr-supermixes-kits?ID=M87F1J8UU">Real-Time PCR Supermixes and Kits</a></h3> <ul> <li>Any instrument &mdash; universal reference dye is compatible with all qPCR platforms</li> <li>Any chemistry &mdash; supermixes for SYBR Green or probe-based detection chemistry</li> <li>Any conditions &mdash; our patented Sso7d fusion polymerase guarantees superior qPCR performance under varying and/or challenging conditions</li> </ul> </li> <li><a href="/en-us/product/v3-western-workflow?ID=M08HIB15"><img src="/webroot/web/images/lsr/products/electrophoresis/category_thumb/global/v3-western-workflow-thumb-western-blotting.jpg" alt="V3 Western Workflow" caption="false" width="125" height="81" /></a> <h3><a href="/en-us/product/v3-western-workflow?ID=M08HIB15">V3 Western Workflow</a> &mdash; Visualize, Verify, Validate</h3> <p>A five-step streamlined western blotting protocol with innovative tools including proprietary stain-free imaging:</p> <ul> <li>Faster run and transfer times</li> <li>Checkpoints at every step</li> <li>Quantitation made simple</li> </ul> </li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies?ID=N8TPQQ15" title="PrecisionAb"><img src="/webroot/web/images/lsr/products/gene_transfer_rnai/category_thumb/global/PrecisionAb Validated_Western_Blotting_Antibodies.jpg" alt="PrecisionAb Validated_Western_Blotting_Antibodies" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/product/precisionab-validated-western-blotting-antibodies?ID=N8TPQQ15">PrecisionAb Validated Western Blotting Antibodies</a></h3> <ul> <li>Robust validation &mdash; performed on up to 12 different cell lysates with endogenous protein levels</li> <li>Reproducible data &mdash; stringent batch-to-batch QC</li> <li>High specificity and sensitivity &mdash; low nonspecific binding and strong signal from target proteins</li> </ul> </li> <li><a href="/en-us/product/zoe-fluorescent-cell-imager?ID=N74CIZE8Z"><img src="/webroot/web/images/lsr/products/cell_imaging/product_thumb/global/cell-imaging-thumb-zoe-fluorescent-cell-imager.jpg" alt="qPCR" caption="false" width="125" height="81" /></a> <h3><a href="/en-us/product/zoe-fluorescent-cell-imager?ID=N74CIZE8Z">ZOE Fluorescent Cell Imager</a></h3> <ul> <li>Simplified cell imaging &mdash; the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training</li> <li>Flexible operation &mdash; brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications</li> </ul> </li> </ul> </div> <div id="fragment-6" class="ui-tabs-panel"> <ul class="list-set list-full-col list-set-th"> <li><a href="/en-us/product/cfx-automation-system-ii?ID=KR44O415"><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/cfx-automation-system-optnav.png" alt="CFX Automation System II" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/product/cfx-automation-system-ii?ID=KR44O415">CFX Automation System II</a></h3> <ul> <li>Works with all CFX Real-Time PCR Systems to facilitate automated workflows, large-volume data generation, and rapid data analysis</li> <li>Saves valuable lab space &mdash;&nbsp;run two CFX Systems with a single plate handler in a compact footprint</li> <li>Run up to up to 84 plates per day</li> </ul> </li> <li><a href="/en-us/category/digital-pcr?ID=M9HE2R15"><img src="/webroot/web/images/opt/digital-pcr/ddpcr-prod-nav-systems.png" alt="Digital PCR Systems" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/category/digital-pcr?ID=M9HE2R15">QX200 Droplet Digital PCR System </a></h3> <ul> <li>Detection of small or large edits with absolute quantification</li> <li>Most precise and sensitive digital PCR solution for all applications</li> <li>Digital PCR chemistry optimized for TaqMan hydrolysis probes or EvaGreen assays</li> <li>Flexible assay setup for high sensitivity or high throughput</li> </ul> </li> <li><a href="/en-us/product/ddpcr-genome-edit-detection-assays?ID=PG9ALWE08O1Y"><img src="/webroot/web/images/lsr/products/amplification_pcr/category_thumb/global/ddpcr-Genome-Edit-Detection-assays-detail-trans.png" alt="ddPCR Genome Edit Detection Assays" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/product/ddpcr-genome-edit-detection-assays?ID=PG9ALWE08O1Y">ddPCR Genome Edit Detection Assays</a></h3> <ul> <li>Enable rapid assessment of HDR and NHEJ edits generated by CRISPR-Cas9 system or other targeted genome editing tools</li> <li>Detect editing events present at frequencies &le; 0.5%</li> <li>Provide absolute quantification of genome editing events from as little as 5 ng of total gDNA (cells in a single well of a 96-well plate)</li> </ul> </li> <li><a href="/en-us/product/ze5-cell-analyzer?ID=OC62Q015"><img src="/webroot/web/images/lsr/products/flow_cytometry/category_thumb/global/ze5-cell-analyzer-thumb-flow-cytometry.jpg" alt="Ze5&trade; Cell Analyzer" /></a> <h3><a href="/en-us/product/ze5-cell-analyzer?ID=OC62Q015">ZE5 Cell Analyzer</a></h3> <ul> <li>Analysis of up to 30 parameters from a single sample, expanding the number of biomarkers that can be monitored in a cell-based assay</li> <li>Measures rare or transient cell populations quickly</li> <!--<li>Examine up to 30 parameters simultaneously, allowing rare or transient cell types to be easily analyzed</li> <li>Up to 384 well plates for high throughput capacity</li> <li>Temperature controlled sample loading for sensitive samples</li>--></ul> </li> <li><a href="/en-us/product/v3-western-workflow?ID=M08HIB15"><img src="/webroot/web/images/lsr/products/electrophoresis/category_thumb/global/v3-western-workflow-thumb-western-blotting.jpg" alt="V3 Western Workflow" caption="false" width="125" height="81" /></a> <h3><a href="/en-us/product/v3-western-workflow?ID=M08HIB15">V3 Western Workflow</a> &mdash; Visualize, Verify, Validate</h3> <p>A five-step streamlined western blotting protocol with innovative tools including proprietary stain-free imaging:</p> <ul> <li>Faster run and transfer times</li> <li>Checkpoints at every step</li> <li>Quantitation made simple</li> </ul> </li> <li><a href="/en-us/category/bio-plex-multiplex-immunoassays?ID=O5BUUA15"><img src="/webroot/web/images/lsr/products/gene_transfer_rnai/category_thumb/global/bioplex-assays-kits-finder.png" alt="BPMI" width="125" height="81" caption="false" /></a> <h3><a href="/en-us/category/bio-plex-multiplex-immunoassays?ID=O5BUUA15">Bio-Plex Multiplex Immunoassays</a></h3> <ul> <li>Measure up to 500 unique analytes in a single sample</li> <li>Assay and panels for over 450 biologically relevant targets: inflammation, disease, cancer, cell signaling and growth, apoptosis, toxicity, and more</li> <li>Choose premade or custom configurations, or develop your own assays for new targets</li> </ul> </li> <li><a href="/en-us/category/bio-plex-reader-tools?ID=317582a5-ac11-4e2c-8ee6-4cf0799352fd"><img src="/webroot/web/images/lsr/products/bioplex_suspension_array/category_feature/global/bio-plex-3d-prod-nav.png" alt="Bio-Plex 3D Suspension Array System" caption="false" width="125" height="73" /></a> <h3><a href="/en-us/category/bio-plex-reader-tools?ID=317582a5-ac11-4e2c-8ee6-4cf0799352fd">Bio-Plex Readers and Tools</a></h3> <ul> <li>Premium instruments, software, calibration, and validation kits to ensure consistent, reproducible results</li> <li>Use our Bio-Plex Multiplex Immunoassays, powered by Luminex xMAP Technology</li> <li>Measure up to 500 unique analytes in a single sample</li> </ul> </li> </ul> </div> </div> </div> </div> </div> </div> </div> <div><a name="module4"></a></div> <div class="opt-module optresources-bg no-bttm-pddng"> <h2>Resources</h2> <div id="youtube-videos">&nbsp;</div> <div class="dotteddivider dotteddividerdrk top-margin">&nbsp;</div> <ul class="list-set list-two-col list-set-th"> <li><a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Resources&amp;tgp_pcp_link=Bulletin 6029','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999')" href="/webroot/web/pdf/lsr/literature/Bulletin_6029.pdf" class="no-target-style"><img src="/webroot/web/images/lsr/products/bioplex_suspension_array/category_feature/global/From_megaTALs_to_CRISPR.jpg" alt="From_megaTALs_to_CRISPR" width="85px" height="85px" caption="false" /></a> <h3><a class="no-target-style" onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Resources&amp;tgp_pcp_link=Bulletin 6029','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="https://www.bioradiations.com/from-megatals-to-crispr-the-many-ways-to-edit-a-gene/">From megaTALs to CRISPR: The Many Ways to Edit a Gene</a></h3> </li> <li><a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Resources&amp;tgp_pcp_link=Bulletin 6488','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999')" href="/webroot/web/pdf/lsr/literature/Bulletin_6488.pdf" class="no-target-style"><img src="/webroot/web/images/lsr/products/bioplex_suspension_array/category_feature/global/Erasing_Disease.jpg" alt="Erasing_Disease" width="85px" height="85px" caption="false" /></a> <h3><a class="no-target-style" onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Resources&amp;tgp_pcp_link=Bulletin 6488','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="https://www.bioradiations.com/erasing-disease-how-gene-editing-is-changing-genetic-therapies/">Erasing Disease: How Gene Editing is Changing Genetic Therapies</a></h3> </li> <li><a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Resources&amp;tgp_pcp_link=Bulletin 6518','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999')" href="/webroot/web/pdf/lsr/literature/Bulletin_6518.pdf" class="no-target-style"><img src="/webroot/web/images/lsr/products/bioplex_suspension_array/category_feature/global/CRISPR-Changing_the_Pace_of_BioPharma_R&amp;D.jpg" alt="CRISPR-Changing_the_Pace_of_BioPharma_R&amp;D" width="85px" height="85px" caption="false" /></a> <h3><a class="no-target-style" onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=Resources&amp;tgp_pcp_link=Bulletin 6518','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="https://www.bioradiations.com/crispr-changing-the-pace-of-biopharma-rd/">CRISPR: Changing the Pace of BioPharma R&amp;D</a></h3> </li> </ul> <div class="dotteddivider dotteddividerdrk">&nbsp;</div> <h3 class="opt-subhead30">Documents</h3> <ul class="list-set list-two-col list-set-th"> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_7096.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Speed_Time_to_Results.png" alt="Speed_Time_to_Results" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_7070.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Speed Time to Results by Incorporating Flow Cytometry into the CRISPR-Cas9 Gene Editing Workflow</a> <br /><span class="filesize">(PDF 572&nbsp;KB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_7095.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Publication_List.png" alt="Publication_List" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_6826.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Publication List: Electroporation of Nucleic Acids and Proteins for CRISPR-Related Gene Editing</a> <br /><span class="filesize">(PDF&nbsp;119 KB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_7048.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/CRISPR_Gene_Editing_Workflow.png" alt="CRISPR_Gene_Editing_Workflow" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_6947.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">CRISPR Gene Editing Workflow </a> <br /><span class="filesize">(PDF&nbsp;3.1 MB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6959.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Gene_Editing.png" alt="Gene_Editing" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_7088.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Gene Editing: Clearing the Roadblocks to a New Class of Therapeutics </a> <br /><span class="filesize">(PDF&nbsp;340&nbsp;KB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6516.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Ultra-Sensitive_Quantification_of_Genome.png" alt="Ultra-Sensitive_Quantification_of_Genome" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_6712.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital PCR</a> <br /><span class="filesize">(PDF&nbsp;614 KB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_7079.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Fast_and_Efficient_Workflow.png" alt="Fast_and_Efficient_Workflow" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_7079.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Fast and Efficient Workflow to Monitor CRISPR-Cas9 Gene Editing in Haploid Cell Lines</a> <br /><span class="filesize">(PDF&nbsp;665 KB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6959.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Droplet_Digital_PCR_Applications_Guide.png" alt="Droplet_Digital_PCR_Applications_Guide" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Droplet Digital PCR Applications Guide</a> <br /><span class="filesize">(PDF 3.9 MB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6516.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Reliable_Single-Cell_Sorting.png" alt="Reliable_Single-Cell_Sorting" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_6853.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Reliable Single-Cell Sorting with Bio-Rad's S3e Cell Sorter</a> <br /><span class="filesize">(PDF&nbsp;508 KB)</span></h3> <p></p> </li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_5692.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style"><img src="/webroot/web/images/lsr/solutions/applications/protein_functional_analysis/biomarker_analysis/Real-Time_qPCR_as_a_Tool.png" alt="Real-Time_qPCR_as_a_Tool" width="85" height="110" caption="false" /></a> <h3><a href="/webroot/web/pdf/lsr/literature/Bulletin_5692.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing</a> <br /><span class="filesize">(PDF&nbsp;265 KB)</span></h3> </li> </ul> </div> <script type="text/javascript" src="/webroot/web/js/apptech-opt-conveter-min.js"></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $(function() { $('#rotate > ul').tabs({ fx: { opacity: 'toggle' } }).tabs('', 2000); }); 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