Gene Pulser Xcell Electroporation Systems

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Overview

Gene Pulser Xcell modular electroporation systems include a main unit, two accessory modules, and the ShockPod™ cuvette chamber.

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Description

Description

The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every cell type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system is composed of a main unit, two accessory modules, the capacitance extender (CE module) and the pulse controller (PC module), and a ShockPod cuvette chamber.

Features and Benefits of the Gene Pulser Xcell Electroporator

  • Universal electroporation — transfects all cell types, from primary, stem, and T cells to bacteria and fungi
  • Flexibility with your experiment parameters for the highest transfection efficiency and viability 
    • Preset protocols — ready-to-use preoptimized protocols for frequently used mammalian and bacterial cell types
    • Manual operation — allows for entry and storage of new parameters or editing of all parameters
    • Optimization protocols — enables the best conditions to be determined using incremental voltage steps
    • Exponential and square waveforms — allows for optimal delivery
  • Confidence in your results — uses PulseTrac circuitry and arc-protection features to ensure sample protection and reproducible results
  • Save time optimizing
    • Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells
    • Prevalidated published protocols — saves time optimizing conditions
  • Easily accessed data management — storage and recall of parameters and results from previous 100 experiments, useful for troubleshooting

Electroporation System Options

More Information

  • Features modifiable delivery parameters including time constant, actual voltage applied, pulse interval, and pulse time, depending on the waveform (exponential or square) chosen
  • Provides a choice of programs for manual operation, preset protocols, user protocols, and an optimization protocol
  • Compatible with 0.1 cm, 0.2 cm, and 0.4 cm Gene Pulser/MicroPulser Electroporation Cuvettes
  • Compatible with any electroporation buffer, including Bio-Rad's Gene Pulser Electroporation Buffer

Specifications

Specifications

Gene Pulser Xcell Total System
Includes main unit, CE module, PC module, and ShockPod cuvette chamber
 
Outputs
Waveform: Exponential or square
Voltage: 10–3,000 V
Capacitance
10–500 V, 25–3,275 µF in 25 µF increments
500–3,000 V, 10, 25, 50 µF
Resistance (parallel)
50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance
20 Ω minimum at 10–2,500 V
600 Ω minimum at 2,500–3,000 V
Square-wave timing 
10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval
500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Gene Pulser Xcell Eukaryotic System
Includes main unit, CE module, and ShockPod cuvette chamber
 
Outputs and other specifications
Same as total system without the parallel resistance
Gene Pulser Xcell Microbial System
Includes main unit, PC module, and ShockPod cuvette chamber
 
Outputs
Waveform: Exponential or square
Voltage: 200–3,000 V
Capacitance 
10, 25, 50 µF
Resistance (parallel) 
50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance 
20 Ω minimum at 200–2,500 V
600 Ω minimum at 2,500–3,000 V
Square-wave timing 
0.05–5 ms pulse duration in 0.05 ms increments,
1–2 pulses, 5 sec minimum interval
Gene Pulser Xcell Main Unit
 
Outputs
Waveform: Exponential or square
Voltage: 200–3,000 V
Capacitance
10, 25, 50 µF
Sample resistance
20 Ω minimum at 200–2,500 V
600 Ω minimum at 2,500–3,000 V
Square-wave timing 
0.05–5 ms pulse duration in 0.05 ms increments,
1–2 pulses, 5 sec minimum interval
General
 
Input voltage
100–120 VAC or 220–240 VAC, 50/60 Hz
Power
Maximum 240 W (during short charging periods)
Operating environment
Temperature 0–35°C, humidity 0–95% (noncondensing)
Regulatory
Safety EN 61010, EMC EN 61326 Class A
Dimensions (W x D x H)
Main unit: 31 x 30 x 14 cm
CE module: 31 x 28 x 9 cm
PC module: 31 x 28 x 5 cm
Weight
Main unit: 6.6 kg
CE module: 3.1 kg
PC module: 1.9 kg

Ordering

Ordering

items

Use the filters below to refine results!

165-2660_tn.jpg
1652660
100/240 V, 50/60 Hz, electroporator, includes main unit, CE and PC modules, shocking chamber, 15 sterile electroporation cuvettes (5 each of 0.1, 0.2, and 0.4 cm electrode gap), cuvette rack
165-2661_tn.jpg
1652661
100/240 V, 50/60 Hz, electroporator, includes main unit, CE module for exponential-decay or square-wave pulses, shocking chamber,
5 x 0.4 cm sterile electroporation cuvettes, cuvette rack
165-2662_tn.jpg
1652662
100/240 V, 50/60 Hz, electroporator, includes main unit, PC module for exponential-decay pulse delivery, shocking chamber, 5 each of 0.1 and 0.2 cm sterile electroporation cuvettes, cuvette rack
165-2666_tn.jpg
1652666
100/240 V, 50/60 Hz, main module of the Gene Pulser Xcell electroporation system
165-2667_tn.jpg
1652667
Pkg of 1, capacitance extender for use with the Gene Pulser Xcell electroporation system, functional range of 25–3,275 μF, includes integral leads
165-2668_tn.jpg
1652668
Pkg of 1, pulse controller module for use with the Gene Pulser Xcell electroporation system, range of 50–1,000 ohm, includes integral leads
165-2669_tn.jpg
1652669
Pkg of 1, electroporation chamber of the Gene Pulser Xcell and Gene Pulser II electroporation systems, includes integral leads

Accessories

Accessories

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1652081
Pkg of 5, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
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1652082
Pkg of 5, 0.2 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
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1652083
Pkg of 5, 0.1 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2088_tn.jpg
1652088
Pkg of 50, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2086_tn.jpg
1652086
Pkg of 50, 0.2 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2089_tn.jpg
1652089
Pkg of 50, 0.1cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2091_tn.jpg
1652091
Pkg of 500, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2092_tn.jpg
1652092
Pkg of 500, 0.2–cm gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2093_tn.jpg
1652093
Pkg of 500, 0.1 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2095_tn.jpg
1652095
Pkg of 1, cuvette rack for the Gene Pulser electroporator

Kits & Reagents

Kits & Reagents

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1652676
10 x 1.8 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser electroporation system
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1652677
30 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser Electroporation System

Documents

Documents

Number Description Options
4006217
Instruction Manual, Gene Pulser Xcell Electroporation System, Rev A
10000146903
Update to EN 61010-1 Amendment Statement of Operating Conditions
4006218
Instruction Manual, Gene Pulser Xcell Electroporation System Quick Guide, Rev A
5542
Electroporation Systems Brochure, Rev A
[ Add to Cart (Free) ]
5445
Gene Pulser Xcell Electroporation System Flier, Rev A
10000118182
Gene Pulser Xcell Electroporation Systems Recommended IQ/OQ Protocol
7304
Optimizing Electroporation Conditions to Transfect mRNA CD8+ T Cells with High Efficiency
2249
Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells, Rev A
1360
Efficient Electro-transformation of Mycobacterium smegmatis
1354
Transgenic Zebrafish by Electroporation
1352
Transformation of Filamentous Fungi by High-Voltage Electroporation
1359
High Electro-transformation Efficiencies Obtained With DNA From Ligation Mixtures
1357
Electroporation of Yeast Artificial Chromosomes
1358
Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
1969
Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses
1356
Production of Hybridomas by Electroporation
1355
Production of Hybridomas by Electrofusion
1365
Introducing Proteins Into Cells by Electroporation
1345
Electroporation of Primary Bone Marrow Cells
1349
Electroporation of T-Cell and Macrophage Cell Lines
5582
Gene Pulser Electroporation Buffer Product Information Sheet, Rev A
5399
Gene Pulser siRNA Electroporation References, Rev A