Gene Pulser Xcell Electroporation Systems
Gene Pulser Xcell modular electroporation systems include a main unit, two accessory modules, and the ShockPod™ cuvette chamber.
Overview
The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every cell type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system is composed of a main unit, two accessory modules, the capacitance extender (CE module) and the pulse controller (PC module), and a ShockPod cuvette chamber.
Features and Benefits of the Gene Pulser Xcell Electroporator
- Universal electroporation — transfects all cell types, from primary, stem, and T cells to bacteria and fungi
- Flexibility with your experiment parameters for the highest transfection efficiency and viability
- Preset protocols — ready-to-use preoptimized protocols for frequently used mammalian and bacterial cell types
- Manual operation — allows for entry and storage of new parameters or editing of all parameters
- Optimization protocols — enables the best conditions to be determined using incremental voltage steps
- Exponential and square waveforms — allows for optimal delivery
- Confidence in your results — uses PulseTrac circuitry and arc-protection features to ensure sample protection and reproducible results
- Save time optimizing
- Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells
- Prevalidated published protocols — saves time optimizing conditions
- Easily accessed data management — storage and recall of parameters and results from previous 100 experiments, useful for troubleshooting
Electroporation System Options
- Gene Pulser Xcell Total Electroporation System (1652660) — the complete electroporation system for transfection of both eukaryotic and prokaryotic cells; square-wave and exponential delivery, includes both the CE module, and the PC module
- Gene Pulser Xcell Eukaryotic Electroporation System (1652661) — for the electroporation of most eukaryotic cells, including mammalian cells and plant protoplasts; square-wave delivery, includes the CE module
- Gene Pulser Xcell Microbial Electroporation System (1652662) — for the electroporation of bacteria and fungi, as well as other applications where high-voltage pulses are applied to samples of small volume and high resistance; exponential-wave delivery, includes the PC module
More Information
- Features modifiable delivery parameters including time constant, actual voltage applied, pulse interval, and pulse time, depending on the waveform (exponential or square) chosen
- Provides a choice of programs for manual operation, preset protocols, user protocols, and an optimization protocol
- Compatible with 0.1 cm, 0.2 cm, and 0.4 cm Gene Pulser/MicroPulser Electroporation Cuvettes
- Compatible with any electroporation buffer, including Bio-Rad's Gene Pulser Electroporation Buffer
Related Technologies
Related Products
Specifications
Gene Pulser Xcell Total System
Includes main unit, CE module, PC module, and ShockPod cuvette chamber
Includes main unit, CE module, PC module, and ShockPod cuvette chamber
Outputs
Waveform: Exponential or square
Voltage: 10–3,000 V
Voltage: 10–3,000 V
Capacitance
10–500 V, 25–3,275 µF in 25 µF increments
500–3,000 V, 10, 25, 50 µF
500–3,000 V, 10, 25, 50 µF
Resistance (parallel)
50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance
20 Ω minimum at 10–2,500 V
600 Ω minimum at 2,500–3,000 V
600 Ω minimum at 2,500–3,000 V
Square-wave timing
10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval
500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Gene Pulser Xcell Eukaryotic System
Includes main unit, CE module, and ShockPod cuvette chamber
Includes main unit, CE module, and ShockPod cuvette chamber
Outputs and other specifications
Same as total system without the parallel resistance
Gene Pulser Xcell Microbial System
Includes main unit, PC module, and ShockPod cuvette chamber
Includes main unit, PC module, and ShockPod cuvette chamber
Outputs
Waveform: Exponential or square
Voltage: 200–3,000 V
Voltage: 200–3,000 V
Capacitance
10, 25, 50 µF
Resistance (parallel)
50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance
20 Ω minimum at 200–2,500 V
600 Ω minimum at 2,500–3,000 V
600 Ω minimum at 2,500–3,000 V
Square-wave timing
0.05–5 ms pulse duration in 0.05 ms increments,
1–2 pulses, 5 sec minimum interval
1–2 pulses, 5 sec minimum interval
Gene Pulser Xcell Main Unit
Outputs
Waveform: Exponential or square
Voltage: 200–3,000 V
Voltage: 200–3,000 V
Capacitance
10, 25, 50 µF
Sample resistance
20 Ω minimum at 200–2,500 V
600 Ω minimum at 2,500–3,000 V
600 Ω minimum at 2,500–3,000 V
Square-wave timing
0.05–5 ms pulse duration in 0.05 ms increments,
1–2 pulses, 5 sec minimum interval
1–2 pulses, 5 sec minimum interval
General
Input voltage
100–120 VAC or 220–240 VAC, 50/60 Hz
Power
Maximum 240 W (during short charging periods)
Operating environment
Temperature 0–35°C, humidity 0–95% (noncondensing)
Regulatory
Safety EN 61010, EMC EN 61326 Class A
Dimensions (W x D x H)
Main unit: 31 x 30 x 14 cm
CE module: 31 x 28 x 9 cm
PC module: 31 x 28 x 5 cm
CE module: 31 x 28 x 9 cm
PC module: 31 x 28 x 5 cm
Weight
Main unit: 6.6 kg
CE module: 3.1 kg
PC module: 1.9 kg
CE module: 3.1 kg
PC module: 1.9 kg
Use
For Research Use Only. Not for use in diagnostic procedures.
Ordering
items
Use the filters below to refine results!
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(Discontinued) Gene Pulser Xcell Total System
1652660
100/240 V, 50/60 Hz, electroporator, includes main unit, CE and PC modules, shocking chamber, 15 sterile electroporation cuvettes (5 each of 0.1, 0.2, and 0.4 cm electrode gap)
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(Discontinued) Gene Pulser Xcell Eukaryotic System
1652661
100/240 V, 50/60 Hz, electroporator, includes main unit, CE module for exponential-decay or square-wave pulses, shocking chamber, 5 x 0.4 cm sterile electroporation cuvettes
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(Discontinued) Gene Pulser Xcell Microbial System
1652662
100/240 V, 50/60 Hz, electroporator, includes main unit, PC module for exponential-decay pulse delivery, shocking chamber, 5 each of 0.1 and 0.2 cm sterile electroporation cuvettes
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(Discontinued) Gene Pulser Xcell Main Unit
1652666
100/240 V, 50/60 Hz, main module of the Gene Pulser Xcell electroporation system
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(Discontinued) Gene Pulser Xcell CE Module
1652667
Pkg of 1, capacitance extender for use with the Gene Pulser Xcell electroporation system, functional range of 25–3,275 μF, includes integral leads
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(Discontinued) Gene Pulser Xcell PC Module
1652668
Pkg of 1, pulse controller module for use with the Gene Pulser Xcell electroporation system, range of 50–1,000 ohm, includes integral leads
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(Discontinued) Gene Pulser Xcell ShockPod Cuvette Chamber
1652669
Pkg of 1, electroporation chamber of the Gene Pulser Xcell and Gene Pulser II electroporation systems, includes integral leads
Accessories
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(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.4 cm gap
1652081
Pkg of 5, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
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(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap
1652082
Pkg of 5, 0.2 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
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(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.1 cm gap
1652083
Pkg of 5, 0.1 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
Image
(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.4 cm gap
1652088
Pkg of 50, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
Image
(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap
1652086
Pkg of 50, 0.2 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
Image
(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.1 cm gap
1652089
Pkg of 50, 0.1cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
Image
(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.4 cm gap
1652091
Pkg of 500, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
Image
(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap
1652092
Pkg of 500, 0.2–cm gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
Image
(Discontinued) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.1 cm gap
1652093
Pkg of 500, 0.1 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
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(Discontinued) Gene Pulser Cuvette Rack
1652095
Pkg of 1, cuvette rack for the Gene Pulser electroporator
Kits & Reagents
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(Discontinued) Gene Pulser Electroporation Buffer
1652676
10 x 1.8 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser electroporation system
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(Discontinued) Gene Pulser Electroporation Buffer
1652677
30 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser Electroporation System