Overview
The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every cell type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system is composed of a main unit, two accessory modules, the capacitance extender (CE module) and the pulse controller (PC module), and a ShockPod cuvette chamber.
Features and Benefits of the Gene Pulser Xcell Electroporator
- Universal electroporation — transfects all cell types, from primary, stem, and T cells to bacteria and fungi
- Flexibility with your experiment parameters for the highest transfection efficiency and viability
- Preset protocols — ready-to-use preoptimized protocols for frequently used mammalian and bacterial cell types
- Manual operation — allows for entry and storage of new parameters or editing of all parameters
- Optimization protocols — enables the best conditions to be determined using incremental voltage steps
- Exponential and square waveforms — allows for optimal delivery
- Confidence in your results — uses PulseTrac circuitry and arc-protection features to ensure sample protection and reproducible results
- Save time optimizing
- Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells
- Prevalidated published protocols — saves time optimizing conditions
- Easily accessed data management — storage and recall of parameters and results from previous 100 experiments, useful for troubleshooting
Electroporation System Options
- Gene Pulser Xcell Total Electroporation System (1652660) — the complete electroporation system for transfection of both eukaryotic and prokaryotic cells; square-wave and exponential delivery, includes both the CE module, and the PC module
- Gene Pulser Xcell Eukaryotic Electroporation System (1652661) — for the electroporation of most eukaryotic cells, including mammalian cells and plant protoplasts; square-wave delivery, includes the CE module
- Gene Pulser Xcell Microbial Electroporation System (1652662) — for the electroporation of bacteria and fungi, as well as other applications where high-voltage pulses are applied to samples of small volume and high resistance; exponential-wave delivery, includes the PC module
More Information
- Features modifiable delivery parameters including time constant, actual voltage applied, pulse interval, and pulse time, depending on the waveform (exponential or square) chosen
- Provides a choice of programs for manual operation, preset protocols, user protocols, and an optimization protocol
- Compatible with 0.1 cm, 0.2 cm, and 0.4 cm Gene Pulser/MicroPulser Electroporation Cuvettes
- Compatible with any electroporation buffer, including Bio-Rad's Gene Pulser Electroporation Buffer
Related Technologies
Related Products
Specifications
Includes main unit, CE module, PC module, and ShockPod cuvette chamber
Voltage: 10–3,000 V
500–3,000 V, 10, 25, 50 µF
600 Ω minimum at 2,500–3,000 V
500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Includes main unit, CE module, and ShockPod cuvette chamber
Includes main unit, PC module, and ShockPod cuvette chamber
Voltage: 200–3,000 V
600 Ω minimum at 2,500–3,000 V
1–2 pulses, 5 sec minimum interval
Voltage: 200–3,000 V
600 Ω minimum at 2,500–3,000 V
1–2 pulses, 5 sec minimum interval
CE module: 31 x 28 x 9 cm
PC module: 31 x 28 x 5 cm
CE module: 3.1 kg
PC module: 1.9 kg
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100/240 V, 50/60 Hz, electroporator, includes main unit, CE and PC modules, shocking chamber, 15 sterile electroporation cuvettes (5 each of 0.1, 0.2, and 0.4 cm electrode gap)
100/240 V, 50/60 Hz, electroporator, includes main unit, CE module for exponential-decay or square-wave pulses, shocking chamber, 5 x 0.4 cm sterile electroporation cuvettes
100/240 V, 50/60 Hz, electroporator, includes main unit, PC module for exponential-decay pulse delivery, shocking chamber, 5 each of 0.1 and 0.2 cm sterile electroporation cuvettes
Accessories
Pkg of 5, 0.2 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for bacteria, yeast, mammalian, and other eukaryotic cells
Pkg of 5, 0.1 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for bacteria and yeast
Pkg of 50, 0.2 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for bacteria, yeast, mammalian, and other eukaryotic cells
Pkg of 50, 0.1 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for bacteria and yeast
Pkg of 500, 0.2 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for bacteria, yeast, mammalian, and other eukaryotic cells
Pkg of 500, 0.1 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for bacteria and yeast
Kits & Reagents