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qPCR Supermixes | iQ and SsoFast PCR Supermixes | Resources | Applications & Technologies | Related Products |
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CFX Loyalty ProgramBio-Rad's supermixes can make any qPCR experiment easier, faster, and more
effective. Our real-time PCR supermixes are designed for:
- Any instrument — universal reference dye is compatible with all qPCR platforms
- Any chemistry — supermixes for SYBR Green or probe-based detection chemistry
- Any conditions — our patented Sso7d fusion polymerase* guarantees superior qPCR performance under varying and/or challenging conditions
qPCR Supermixes
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 SsoAdvanced Universal SYBR Green Supermix |
 SsoAdvanced Universal Probes Supermix |
 SsoAdvanced Universal Inhibitor-Tolerant Supermix |
 iTaq Universal SYBR Green Supermix |
 iTaq Universal Probes Supermix |
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| Features |
Robust performance with demanding templates and under challenging conditions |
Optimized for crude samples containing PCR inhibitors |
Advanced formulation for reliable, reproducible gene expression analysis on any qPCR instrument |
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| Detection chemistry | SYBR Green | Probes | SYBR Green | SYBR Green | Probes |
| Sso7d fusion polymerase | Yes | Yes | Yes | No | No |
| Sensitive detection of low-abundance targets |  |
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| Efficient amplification of difficult targets | |
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| Specific amplification of targets | |
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| Robust performance under varying PCR conditions** | |
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| Tolerance to PCR inhibitors | |
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| Cycling protocol compatibility | Both standard and fast | ||||
| Instrument compatibility | Any instrument | ||||
| Documents | |||||
** Primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)
iQ and SsoFast PCR Supermixes
iQ and SsoFast PCR Supermixes have been supporting researchers performing basic real-time PCR analysis for years. If you have not yet used them, we recommend trying our new SsoAdvanced Universal SYBR Green and SsoAdvanced Universal Probes Supermixes as superior-performance alternatives for use on any real-time PCR instrument.
Supermixes for Dye-Based qPCR Assays
Supermixes for Probe-Based qPCR Assays
Resources
Understanding Real-Time PCR Supermixes
This interactive tutorial provides an introduction to the chemistry behind real-time PCR reactions and supermixes as well as a protocol for comparing different reagents.
Amplification Reagents and Plastics Brochure
(PDF 6.1 MB)
Reagent Comparison Guide for Real-Time PCR
(PDF 3 MB)
Videos
Applications & Technologies
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
Real-Time PCR Data Analysis
An overview of our CFX Manager™ Real-Time PCR Analysis Software features, including high resolution melt (HRM) analysis capabilities
Related Products
PCR Reagents and qPCR Reagents
A wide range of reagents for reverse transcription, PCR, and real-time PCR, optimized to generate accurate and reproducible data
One-Step RT-qPCR from RNA or Cells
For robust reverse transcription and SYBR Green or probe-based qPCR in one step on any instrument
cDNA Synthesis Kits
Reverse transcription kits for easy setup, high reproducibility, and bias-free cDNA synthesis using an optimum blend of primers
Cell Lysis RT-qPCR Kits
Complete and fast solution for one- or two-step qPCR reactions directly from cell lysates with no RNA purification step
Real-Time PCR Detection Systems
An automation-compatible, gradient-enabled thermal cycler with interchangeable optical reaction modules for singleplex or multiplex real-time PCR
Preamplification Supermix
Ready-to-use supermix for unbiased, target-specific preamplification of cDNA or gDNA using up to 100 qPCR assays
PCR Plastic Consumables
A large selection of thin-wall polypropylene PCR tubes, PCR plates, seals, and accessories precisely manufactured for various PCR applications