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On This Page |
Digital PCR Assays & Kits |
Digital PCR Systems, Software, & Consumables |
Droplet Digital PCR Technology |
Droplet Digital PCR Benefits |
Droplet Digital PCR Applications & Publications |
Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low-abundance targets, targets in complex backgrounds, allelic variants (SNPs), and for monitoring subtle changes in target levels that cannot be detected with real-time PCR. This technology is now approved for use in in vitro diagnostics.
Digital PCR Assays and Kits
Bio-Rad offers a comprehensive portfolio of Digital PCR Assays and Kits for numerous applications including Mutation Detection, Copy Number Determination, Genome Edit Detection, Gene Expression, Expert Design Assays, Residual DNA Quantification and Library Quantification.
Digital PCR Systems, Software, and Consumables
Droplet Digital PCR Systems
Bio-Rad’s Droplet Digital PCR Systems provide ultrasensitive and absolute nucleic acid quantification.
QX ONE Droplet Digital PCR (ddPCR) System
A high throughput multiplexed and integrated droplet digital PCR system
QX200 AutoDG Droplet Digital PCR System
For high-throughput analysis
QX200 Droplet Digital PCR (ddPCR) System
For analyzing up to 96 samples
Droplet Digital PCR System Consumables
Purchase droplet generation oil, cartridges, gaskets, plates & seals, plus pipette tips, and more, designed for Droplet Digital PCR Systems.
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Droplet Digital PCR System Supermix
One-Step RT-ddPCR Advanced Kit for Probes for use with the COVID-19 ddPCR Assay.
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QX Software for Digital PCR Systems
QX Software is a family of powerful software packages for acquisition and analysis of your Droplet Digital PCR (ddPCR) data. When connected to a ddPCR System, QX Software provides all necessary functionality to create, run, and analyze experiments on your samples.
Digital PCR Consumables and Reagents
Purchase supermixes, droplet generation oil, cartridges, gaskets, plates & seals, plus pipette tips, and more, designed for Droplet Digital PCR Systems.
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ddPCR Supermixes
For creating sample-in-oil droplets. -
ddPCR Oils
For creating sample-in-oil droplets. -
ddPCR Consumables
For use with the droplet generator. -
PX1 Plate Sealer
For secure plate sealing.
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RainDance Reagents and Consumables
For use with RainDrop, ThunderBolts, and ThunderStorm Systems
QXDx Products for In Vitro Diagnostics (IVD)
IVD Instrument
CE-IVD Instruments
QXDx AutoDG ddPCR System
- First FDA-cleared ddPCR system for use with QXDx BCR-ABL %IS Kit
- Ultra-low quantification without standard curves
- Droplet partitioning reduces amplification bias
- Automated droplet generator, 96-reaction throughput
- CE-IVD marked ddPCR system for use with the QXDx BCR-ABL %IS Kit
- Ultra-low quantification without standard curves
- Droplet partitioning reduces amplification bias
- Automated droplet generator, 96-reaction throughput
QXDx ddPCR System
- CE-IVD marked ddPCR system for use with the QXDx BCR-ABL %IS Kit
- For analyzing up to 96 samples
IVD Consumables, Assays, and Kits
Kits
QXDx Universal Kit for ddPCR System
Reagents and consumables for the IVD ddPCR System
QXDx Universal Kit for AutoDG ddPCR System
Reagents and consumables for the automated IVD ddPCR System
QXDx AutoDG Consumable Pack & QXDx Droplet Reader Oil Pack
Reagents and consumables for the automated IVD ddPCR System.
Assays
QXDx BCR-ABL %IS Kit
An FDA-cleared/CE-marked digital PCR test kit intended to monitor patients with Chronic Myeloid Leukemia (CML) with reported results in both International Scale and MR values.
Droplet Digital PCR Technology
Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.
Read more about Droplet Digital PCR technology or learn how to transition your assay from qPCR to ddPCR.
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DNA sample with target sequence is partitioned into droplets
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Target and background DNA are randomly distributed among 20,000 droplets
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Target sequence is amplified by end-point PCR in each droplet
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Positive droplets are counted to give precise quantification of target sequences in sample
Benefits of Droplet Digital PCR
Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves
Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR
High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps
Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR
Explore Absolute Quantification with Droplet Digital PCR, download the White Paper on Molecular Counting.
Applications of Droplet Digital PCR
Copy number variation (CNV)
Accurate CNV quantification in single wells.
Next generation sequencing
Amplification and accurate quantification of NGS libraries with validation of sequencing results.
Detection of rare sequences in cancer and disease
Detection of mutations and viral load below levels detectable by current tests.
Gene expression analysis
Detection of rare mRNAs and miRNAs with rapid turnover.
Cell & Gene Therapy
ddPCR assays for a range of applications including CAR transgene quantification.
Pathogen detection and microbiome analysis
Viral load and pathogen detection in organ transplants, soil, and food.
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments.
Droplet Digital PCR Publications
Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research featuring Bio-Rad products.
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