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Digital PCR Systems and Consumables |
Droplet Digital PCR Technology |
Benefits of Droplet Digital PCR |
Applications & Technologies |
Droplet Digital PCR Publications |
Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low-abundance targets, targets in complex backgrounds, allelic variants (SNPs), and for monitoring subtle changes in target levels that cannot be detected with real-time PCR. This technology is now approved for use in in vitro diagnostics.
Digital PCR Systems and Consumables
Droplet Digital Systems
QX200 AutoDG Droplet Digital PCR System
For high-throughput analysisQX200 Droplet Digital PCR (ddPCR) System
For analyzing up to 96 samples
Digital PCR Reagents and Assays
ddPCR Supermixes
For sensitive DNA target detectionddPCR Assays
Wet-lab validated and instant assay design for mutation detection, copy number determination, gene expression, genome edit detection, and other advanced applicationsddPCR Kits
Multiplex mutation screening kits for common cancer mutations, residual DNA quantification, copy number and more
Digital PCR Accessories
ddPCR Oils
For creating sample-in-oil dropletsddPCR Consumables
For use with the droplet generatorPX1 Plate Sealer
For secure plate sealing
QXDx Products for In Vitro Diagnostics (IVD)
QXDx ddPCR System
Manual droplet generation
QXDx Universal Kit for ddPCR System
Reagents and consumables for the IVD ddPCR System
QXDx AutoDG ddPCR System
Automated high-throughput analysis
QXDx Universal Kit for AutoDG ddPCR System
Reagents and consumables for the automated IVD ddPCR System
QXDx™ Products for In Vitro Diagnostics (IVD)
Instruments
QXDx ddPCR System
Manual droplet generation
QXDx AutoDG ddPCR System
Automated high-throughput analysis
Kits
QXDx Universal Kit for ddPCR System
Reagents and consumables for the IVD ddPCR System
QXDx Universal Kit for AutoDG ddPCR System
Reagents and consumables for the automated IVD ddPCR System
QXDx AutoDG Consumable Pack and QXDx Droplet Reader Oil Pack
Reagents and consumables for the automated IVD ddPCR System
Assays
QXDx BCR-ABL %IS Kit
An FDA-cleared/CE-marked digital PCR test kit intended to monitor patients with Chronic Myeloid Leukemia (CML) with reported results in both International Scale and MR values
Single-Cell NGS Sample Preparation
Instruments
ddSEQ Single-Cell Isolator
The ddSEQ Single-Cell Isolator is part of the Illumina Bio-Rad Single-Cell Sequencing Solution, the most comprehensive NGS workflow for single-cell gene expression studies.
Kits
SureCell ATAC-Seq Library Prep Kit
Highly sensitive, single-cell ATAC-Seq assay for genome-wide open chromatin interrogation.
SureCell WTA 3' Library Prep Kit
Robust and scalable solution that allows transcriptome profiling of single cells.
Droplet Digital PCR Technology
Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.
Read more about Droplet Digital PCR technology.
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DNA sample with target sequence is partitioned into droplets
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Target and background DNA are randomly distributed among 20,000 droplets
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Target sequence is amplified by end-point PCR in each droplet
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Positive droplets are counted to give precise quantification of target sequences in sample
Benefits of Droplet Digital PCR
Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves
Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR
High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps
Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR
Applications of Droplet Digital PCR
Copy number variation (CNV)
Accurate CNV quantification in single wells
Next generation sequencing
Amplification and accurate quantification of NGS libraries with validation of sequencing results
Detection of rare sequences in cancer and disease
Detection of mutations and viral load below levels detectable by current tests
Gene expression analysis
Detection of rare mRNAs and miRNAs with rapid turnover
Pathogen detection and microbiome analysis
Viral load and pathogen detection in organ transplants, soil, and food
Single cell analysis
High sensitivity make it a robust tool for single-cell analyses
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments.
Droplet Digital PCR Publications
Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research featuring Bio-Rad products.
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