TransFectin™ Lipid Reagent

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Overview

TransFectin reagent is a cationic lipid reagent that delivers the highest efficiencies and expression levels over a broad range of cell lines, including some difficult-to-transfect and primary cells.
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Description

Description

TransFectin reagent is a cationic lipid reagent that delivers the highest efficiencies and expression levels over a broad range of cell lines, including some difficult-to-transfect and primary cells, while minimizing damaging cytotoxic effects observed with other high-efficiency general transfection reagents. It provides high transfection efficiencies with lower amounts of lipid than most other lipid reagents, allowing more experiments per vial of reagent and lower costs per transfection..

TransFectin lipid reagent is a mixture of a proprietary cationic compound and a co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine). These compounds have been optimized for intracellular delivery of nucleic acids into cultured mammalian cells in the presence of serum at cell densities from 40 to >90%. For most cell lines, high levels of expression can be obtained using concentrations of TransFectin reagent and DNA suggested for given sizes of plates/wells.

Key Features

  • Effectiveness — yields high-efficiency transfections and transgene activity for a broad range of cells
  • Low cytotoxicity — helps maintain cell viability
  • Flexibility — excellent performance at culture densities between 40 and 90% and in the presence or absence of serum-containing medium
  • Simplicity — 3-step protocol and no posttransfection media changes required for most cell types

Other Lipid Reagents

Bio-Rad offers a family of lipid transfection reagents, each of which is optimal for certain applications. TransFectin lipid reagent is a general-purpose lipid that is ideal for plasmid delivery. siLentFect™ lipid reagent is an RNAi-specific lipid that is ideal for siRNA delivery. It can also be used to perform cotransfection with a small reporter-plasmid along with the siRNA molecule.

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1703350
0.5 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery
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1703351
1.0 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery
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1703352
5 x 1.0 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery

Documents

Documents

Number Description Options
2873
TransFectin Lipid Reagent Brochure, Rev A
2874
TransFectin Lipid Reagent Flier, Rev B
4106254
Instruction Manual, TransFectin Lipid Reagent, Rev A
5343
Transfection Reagent References, Rev C
3197
Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types, Rev A
3138_015
TransFectin Lipid Reagent Protocol, Human, A459, Lung Carcinoma
3138_005
TransFectin Lipid Reagent Protocol, Rat, PC12, Pheochromocytoma
3138_009
TransFectin Lipid Reagent Protocol, Human, 143B, Bone Marrow Osteosarcoma
5226
Highly Efficient Transfection of Mouse ES Cells With TransFectin Lipid Reagent
3138_018
TransFectin Lipid Reagent Protocol, Human, HEK 293, Kidney
3138_017
TransFectin Lipid Reagent Protocol, Human, HEK 293T, Kidney