Description
Description
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ddPCR Genome Edit Detection Assays offer a fast, precise, simple, and cost-effective method for detection of genome editing events created using nucleases that cause double-stranded breaks in the DNA such as CRISPR-Cas9. Assays to detect HDR (Homology Directed Repair) and NHEJ events (Non-homologous end joining events) can be obtained for any target using Bio-Rad’s easy-to-use, intuitive Digital Assay site.
Droplet Digital PCR offers a superior ultrasensitive, rapid, and low-cost method for absolute quantification of genome editing events, from very small amounts of starting material.
Key Benefits
• Enable rapid assessment of HDR and NHEJ edits generated by CRISPR-Cas9 or other genome editing tools
• Detect editing events present at frequencies ≤ 0.5%
• Provide absolute quantification of genome editing events from as little as 5ng of total gDNA (cells from a single well of a 96 well plate)
• Distinguish between homozygous and heterozygous edits in clonal populations
• Results are easily analyzed using QX Manager Software
Sample Data
Fig. 1. HDR assay readout.HDR mutation positive control (WT + 20% gblocks gene fragment containing a single base pair substitution) with HDR GED + HDR REF Assay. The HDR edits are FAM+HEX-, while the HEX+ and FAM+HEX+ positive clusters represent the total number of copies, including WT, NHEJ, and HDR edits.
Fig. 2. NHEJ assay readout.NHEJ mutation-positive control (WT + 1% gblocks gene fragment containing a one base pair deletion at the predicted cut site). The WT cluster is positive for both FAM and HEX, while the NHEJ mutant single-positive droplets are positive for FAM only.
Packaging Options
Genome edit detection assays, optimized specifically for ddPCR, can be ordered instantly for any target of interest on Bio-Rad’s easy to use Digital Assay Site.
| Catalog# | Product Name | Reaction Size |
| 12002314 | ddPCR NHEJ Gene Edit Assay, 100 rxns | 100 |
| 12002315 | ddPCR NHEJ Gene Edit Assay, 500 rxns | 500 |
| 12003794 | ddPCR NHEJ Gene Edit Pkg, 1000 rxns | 1000* |
*Sequences of primers and probes provided
| Catalog# | Product Name | Reaction Size |
| 12002312 | ddPCR HDR Gene Edit Assay, 100 rxns | 100 |
| 12002313 | ddPCR HDR Gene Edit Assay, 500 rxns | 500 |
| 12003796 | ddPCR HDR Gene Edit Pkg, 1000 rxns | 1000* |
*Sequences of primers and probes provided
| Catalog# | Product Name | Reaction Size |
| 12003805 | ddPCR HDR Ref Assay, Predesigned, 100rxns | 100 |
| 12003806 | ddPCR HDR Ref Assay, Predesigned, 500rxns | 500 |
| 12003793 | ddPCR HDR Ref Pkg, Predesigned, 1000rxns | 1000* |
*Sequences of primers and probes provided
Related Products
Resources
Droplet Digital PCR Applications Guide
Read in-depth information about setting up experiments for ddPCR applications.
ddPCR Genome Edit Detection Assays
ddPCR Genome Edit Detection Assays