Perform true CRISPR gene editing in your laboratory class!
CRISPR gene editing is revolutionizing scientific research. Now your students can do real CRISPR-Cas9 gene editing in the classroom using Bio-Rad's educational CRISPR gene editing kits.
- Out of the Blue CRISPR Kit — apply CRISPR-Cas9 technology to disrupt the lacZ gene in E. coli and produce a phenotype that can be readily visualized through blue-white screening
- Out of the Blue CRISPR Genotyping Extension — (optional) confirm the edit at the molecular level using PCR and agarose gel electrophoresis
Features and Benefits
- Hands-on, true CRISPR gene editing — students get direct experience with this powerful technology
- Visible phenotype — results can be observed with the naked eye in as little as 22 hr
- Safe — familiar and common reagents and non-pathogenic, non-biofilm-forming bacteria generate a phenotype that is common to molecular biology
- Accessible — does not require specialized equipment or techniques
- Comprehensive, supportive curriculum — includes background about the components of a CRISPR-Cas9 system, the science — and math — behind its accuracy and flexibility, and the guided bioinformatic analysis of CRISPR targets students design themselves
Modeling the components of the CRISPR-Cas9 System
Use a paper model to understand the components of the CRISPR-Cas9 system and the math behind its accuracy
lacZ CRISPR Gene Editing Laboratory
Use CRISPR-Cas9 to edit the lacZ gene in E. coli
Genotyping Laboratory (Optional)*
Use PCR and gel electrophoresis to confirm the edit in transformants
Identification and Bioinformatic Analysis of Cas9 Target Sites in Human Disease
Use bioinformatics to design Cas9 target sites in the human genome and determine risk for off-target effect
*Requires purchase of the Out of the Blue Genotyping Extension
Learn More About CRISPR Gene Editing
Appreciating the unique capabilities and limitations of CRISPR enriches the conversation around its applications and ethics in research, agriculture, and medicine. Additional resources can be found below.
CRISPR Gene Editing Resources »
What Will You Teach with CRISPR?
With applications across medicine, agriculture, and beyond, what is the true promise of CRISPR? Should its use be regulated? What are the laws, ethical considerations, and practical limitations to this technology?
Download the infographic
What Will You Need to Do CRISPR?
The Out of the Blue CRISPR Kit provides all the plasmids, bacterial strains, culture reagents, and plastics to perform CRISPR gene editing in the classroom. Each kit supports 32 students or 8 student workstations; reagent refill packs are also available.
See the Lab Preparation Checklist for information about all required recommended supplies.
For the Out of the Blue CRISPR Kit
For the Out of the Blue Genotyping Extension
If you are a life science educator, you may qualify for a special discounted price (EDU price) on Bio-Rad’s Life Science Education kits, equipment, and reagents. The discount applies to products used for educational purposes only in K–12 and undergraduate classrooms.
Contact Customer Care to request an account number.