High-Fidelity PCR Kits
High-fidelity PCR reagents featuring the thermostable iProof Polymerase. This enzyme utilizes Sso7d fusion polymerase technology to provide highly accurate amplification of targets up to 37 kb.
Overview
iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.
Features and Benefits of iProof High-Fidelity DNA Polymerase:
- High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
- Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
- Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)
Choose the best DNA polymerase for your application.
| Key Features |
|
|
|---|---|---|
| Speed |  |
 |
| Processivity | |
|
| Long fragment amplification | |
|
| Proofreading | Yes | No |
| Hot-Start | No | Yes |
Related Categories
- PCR Core Reagents (PCR Buffers & Reagents)
- RNA Isolation Kits
- Reverse Transcription Kits
- Real-time PCR Supermixes
Supporting Documentation
- iProof High-Fidelity PCR Kit Instruction Manual
- iProof High-Fidelity Master Mix Instruction Manual
- Amplification Reagents and Plastics Brochure
Specifications
Storage at –20°C
iProof™ Enzyme, Kit, and Buffers: guaranteed for 6 months at –20°C in a constant-temperature freezer
iProof Master Mixes: guaranteed for 3 months at –20°C in a constant-temperature freezer (multiple freeze/thaws not recommended)
iProof Master Mixes: guaranteed for 3 months at –20°C in a constant-temperature freezer (multiple freeze/thaws not recommended)
Storage at +4°C
Guaranteed for 3 months at 4°C
Ordering
items
Use the filters below to refine results!
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(Discontinued) iProof HF Master Mix, 100 x 50 µl rxns, 2.5 ml
1725310
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates
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(Discontinued) iProof GC Master Mix, 100 x 50 µl rxns, 2.5 ml
1725320
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates
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(Discontinued) iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl
1725301
100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
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(Discontinued) iProof™ High-Fidelity DNA Polymerase, 500 U (2 U/µl), 250 µl
1725302
500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
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(Discontinued) iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl
1725330
50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard
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(Discontinued) iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl
1725331
200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard
Documents
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Number Description Options
10002300
Instruction Manual, iProof High-Fidelity PCR Kit, Rev B
10002298
Instruction Manual, iProof High-Fidelity DNA Polymerase, Rev B
5211
iProof High-Fidelity DNA Polymerase Flier, Rev C
6090
Amplification Reagents and Plastics Brochure
5337
Long Inverse PCR Using iProof Polymerase, Rev A
10000083948
Instruction Manual, iProof™ High-Fidelity PCR Master Mix