QX200 Droplet Digital PCR System
Bio-Rad’s second-generation digital PCR system provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan Hydrolysis Probes. Unmatched sensitivity and precision for various applications.
Overview
QX Software for Digital PCR Systems
When connected to a ddPCR System, QX Software provides all necessary functionality to create, run, and analyze experiments on your samples.
Learn More About QX Software for Digital PCR Systems
Buy Digital PCR Assays
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Droplet Digital PCR Publications
Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research featuring Bio-Rad products.
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Benefits of the QX200 Droplet Digital PCR System
- Most precise and sensitive digital PCR solution for a wide variety of applications
- Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
- Flexible assay setup — scalable for high sensitivity or high throughput
- Simple and easy-to-use workflow with 96-sample throughput
- Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
- Convenient assay design — standard curves are not required
See performance data for the QX200 Droplet Digital PCR System.
Watch our Success Stories videos to learn more about the applications of the Droplet Digital System in diverse research fields.
View our Droplet Digital PCR reference library with links to scientific journal articles, conference posters, BioRadiations articles and Bio-Rad technical reports.
Applications of the QX200 Droplet Digital PCR System
- Cancer biomarker studies and copy number variation
Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. PrimePCR ddPCR Assays are now available for mutation and copy number detection. These are predesigned, wet-lab validated assays. - Pathogen detection
Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring. - Next generation sequencing
Perform accurate quantification and qualification of NGS libraries without the use of a standard curve. - Gene expression analysis
Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA. - Environmental monitoring
Test a wide variety of environmental samples like soil and water using the QX200 System. - Food testing
Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:
- Copy number variation analysis
- Rare mutation and sequence detection
- Gene expression
- Next-generation sequencing library analysis
Droplet Digital PCR Technology and Workflow
The QX200 Droplet Digital PCR System consists of two instruments, the QX200 Droplet Generator and the QX200 Droplet Reader, and their associated consumables.
The QX200 Droplet Generator is used to partition ddPCR reaction mix into thousands of nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 Droplet Reader. Droplets are read well by well as they are streamed single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.
Additional Instruments
The QX200 Droplet Digital PCR System requires the use of the PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.
Required Accessories
ddPCR Reagents for Hydrolysis Probe-Based Applications
- PrimePCR ddPCR Assays
- ddPCR Supermixes for Probes (No dUTP)
- ddPCR Supermix for Probes
- One-Step RT-ddPCR Advanced Kit for Probes
- Droplet Generation Oil for Probes
- Droplet Reader Oil or ddPCR Droplet Reader Oil EcoTank for Probes
ddPCR Reagents for EvaGreen Applications
More Information
Explore the features of the QX200 System in more depth in the QX200 Droplet Digital PCR System Brochure (bulletin 6311) or contact your Bio-Rad sales representative for more information.
Bio-Rad's Certified Service Provider Program for Droplet Digital PCR
Bio-Rad's Certified Service Providers allow any lab to access high-quality, validated analyses using our proprietary technology. Learn More
Specifications
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Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
QX600 and QX200 System control and analysis software, with traceability for secure environments
Accessories
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
96-well, thermal cycler for PCR with cables; for research use only.
Kits & Reagents
1.2 ml (2 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
3 ml (5 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
12.5 ml (5 x 2.5 ml), 4x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
200 x 20 µl reactions, includes ddPCR Library Quantification Assay and ddPCR Supermix for Probes (No dUTP), for quantification of Illumina TruSeq libraries using the QX200™/QX100™ ddPCR™ Systems
Consumables
Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX600/QX200 Droplet Digital™ PCR Systems
70 ml (10 x 7 ml), oil for use with droplet generator in the QX600/QX200 Droplet Digital™ PCR Systems
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 24, cartridges for use with the QX200 Droplet Generator
Pkg of 24, gaskets for use with QX200 Droplet Generator
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200 Droplet Generator
