QX200 Droplet Digital PCR System

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Overview

Bio-Rad’s second-generation digital PCR system provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan Hydrolysis Probes. Unmatched sensitivity and precision for various applications.

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Description

Description

 

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Benefits of the QX200 Droplet Digital PCR System

  • Most precise and sensitive digital PCR solution for a wide variety of applications
  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Flexible assay setup — scalable for high sensitivity or high throughput
  • Simple and easy-to-use workflow with 96-sample throughput
  • Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required

See performance data for the QX200 Droplet Digital PCR System.

Watch our Success Stories videos to learn more about the applications of the Droplet Digital System in diverse research fields.

View our Droplet Digital PCR reference library with links to scientific journal articles, conference posters, BioRadiations articles and Bio-Rad technical reports.

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation
    Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution.
  • Pathogen detection
    Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
  • Next generation sequencing
    Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.
  • Gene expression analysis
    Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
  • Environmental monitoring
    Test a wide variety of environmental samples like soil and water using the QX200 System.
  • Food testing
    Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:

  • Droplet Digital PCR applications guide
    • Copy number variation analysis
    • Rare mutation and sequence detection
    • Gene expression
    • Next-generation sequencing library analysis

 

Droplet Digital PCR Technology and Workflow

 

The QX200 Droplet Digital PCR System consists of two instruments, the QX200 Droplet Generator and the QX200 Droplet Reader, and their associated consumables.

The QX200 Droplet Generator is used to partition ddPCR reaction mix into thousands of nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 Droplet Reader. Droplets are read well by well as they are streamed single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.

QX200 Droplet Digital PCR system workflow

Additional Instruments

The QX200 Droplet Digital PCR System requires the use of the C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.

Required Accessories

ddPCR Reagents for Hydrolysis Probe-Based Applications

ddPCR Reagents for EvaGreen Applications

More Information

Explore the features of the QX200 System in more depth in the QX200 Droplet Digital PCR System Brochure (bulletin 6311) or contact your Bio-Rad sales representative for more information.

Specifications

Specifications

Starting sample size, µl
20
QX200 droplet generator capacity
1–8 samples/cartridge
Droplets per 20 µl sample
20,000
QX200 droplet reader capacity
1–96 samples
Sample illumination
Light-emitting diodes
Sample detection
Multi-pixel photon counter
Detection channels
FAM (EvaGreen), HEX (VIC)
Linear dynamic range
5 orders of magnitude
Precision
±10%
Droplets per 96-well plate, million
~1.5
QX200 droplet generator dimensions (W x D x H)
28 x 36 x 13 cm (11 x 14 x 5")
QX200 droplet reader dimensions (W x D x H)
66 x 52 x 29 cm (26 x 20 x 11")

Ordering

Ordering

items

Use the filters below to refine results!

qx200-ddpcr-system-186-4001-thumb.jpg
1864001

Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications

qx200-droplet-generator-186-4002-thumb.jpg
1864002

Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System

qx200-droplet-reader-186-4003-thumb.jpg
(Discontinued) QX200 Droplet Reader
1864003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
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12012172
QX200 System Control and Analysis Software, with traceablity for secure environments

Accessories

Accessories

PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
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1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

Kits & Reagents

Kits & Reagents

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12005909

1.2 ml (2 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems

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12005910

3 ml (5 x 0.6 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems

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12005911
12.5 ml (5 x 2.5 ml), 4x supermix, for use in sample preparation for droplet generation in the QX200 Droplet Digital PCR Systems
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1863023

2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems

qx100-droplet-pcr-supermix-5ml-186-3024-thumb.jpg
1863024

5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems

qx100-droplet-pcr-supermix-25ml-186-3025-thumb.jpg
1863025
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems
qx100-ddpcr-supermix-probes-2ml-186-3026-thumb.jpg
1863026
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-supermix-probes-5ml-186-3010-tn.jpg
1863010
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-supermix-probes-25ml-186-3027-tn.jpg
1863027
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-supermix-probes-50ml-186-3028-thumb.jpg
1863028
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
one-step-rt-ddpcr-advanced-kit-for-probes-1864021-thumb.jpg
1864021

200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

one-step-rt-ddpcr-advanced-kit-for-probes-1864022-thumb.jpg
1864022

500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

qx100-ddpcr-buffer-control-kit-186-3052-thumb.jpg
1863052
9 ml (2 x 4.5 ml), 2x buffer, for use as blank control with Droplet Generation Oil for Probes
qx200-ddpcr-evagreen-supermix-186-4033-thumb.jpg
1864033
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx200-evagreen-ddpcr-supermix-186-4034-thumb.jpg
1864034

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

qx200-evagreen-ddpcr-supermix-186-4035-thumb.jpg
1864035
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx200-evagreen-ddpcr-supermix-186-4036-thumb.jpg
1864036
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx100-ddpcr-buffer-control-kit-186-3052-thumb.jpg
1864052
9 ml (2 x 4.5 ml), 2x buffer, for use as a blank control with Droplet Generation Oil for EvaGreen
ddpcr-library-quantification-kit-for-ilumina-truseq-186-3040-thumb.jpg
1863040

200 x 20 µl reactions, includes ddPCR Library Quantification Assay and ddPCR Supermix for Probes (No dUTP), for quantification of Illumina TruSeq libraries using the QX200™/QX100™ ddPCR™ Systems

Consumables

Consumables

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12001925

Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX200™/QX100™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

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1863005
70 ml (10 x 7 ml), oil for use with droplet generator in the QX200/QX100™ Droplet Digital™ PCR Systems
qx200-droplet-generation-oil-for-evagreen-assays-186-4005-thumb.jpg
1864005
14 ml (2 x 7 ml), oil for use with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx200-droplet-generation-oil-for-evagreen-assays-186-4006-thumb.jpg
1864006
70 ml (10 x 7 ml), oil for use with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx100-droplet-reader-oil-2l-186-3004-thumb.jpg
1863004

2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems

dg8-cartridges-for-qx100-qx200-droplet-generator-186-4008-thumb.jpg
1864008
Pkg of 24, cartridges for use with the QX200™/QX100™ Droplet Generator
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1863009
Pkg of 24, gaskets for use with QX200™/QX100™ Droplet Generator
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1864007
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200™/QX100™ Droplet Generator
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(Discontinued) DG8 Cartridge Holder
1863051
Pkg of 1, cartridge holder, positions and holds the cartridge in the QX200 Droplet Generator
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1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Documents

Documents

Number Description Options
7250
From Discovery to Manufacturing: A Workflow for Protein-Based Immunotherapies
7111
Fast, Accurate, and High-Sensitivity Virus Detection: Zika and Ebola Assays for Droplet Digital PCR Systems
6718
Guidelines for Phasing Variants Using Bio-Rad's Droplet Digital PCR System Application Note
6311
QX200 Droplet Digital PCR System Brochure, Rev C
[ Add to Cart (Free) ]
10000107223
QX Manager Software Standard Edition, User Guide
10000107224
QX Manager Software Regulatory Edition, User Guide
10031907
Instruction Manual, QX200™ Droplet Generator, Rev C
10023997
Instruction Manual, PX1 PCR Plate Sealer, Rev A
10021377
Instruction Manual, C1000 Touch Thermal Cycler, Rev B
6512
PrimePCR Assays for Droplet Digital PCR Brochure, Rev A
[ Add to Cart (Free) ]
6782
ddPCR™ Multiplex Mutation Screening Kits Flier, Ver D
[ Add to Cart (Free) ]
6338
Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A
[ Add to Cart (Free) ]
6473
QX200 ddPCR EvaGreen Supermix Flier, Rev A
6250
One-Step RT-ddPCR Kit for Probes Flier, Rev A
6402
ddPCR Library Quantification Kit for Illumina TruSeq Flier, Rev B
6721
ddPCR™ Supermix for Residual DNA Quantification Flier, Rev A
6490
ddPCR Library Quantification Kit for Ion Torrent Flier, Rev A
6407
Droplet Digital PCR Applications Guide
6343
QX100 Droplet Digital PCR System for Accurate Quantification of Illumina TruSeq Libraries, Rev B
6495
Bio-Rad’s ddPCR™ Library Quantification Kit for Ion Torrent Enables Accurate Library Quantification and Excellent Balancing of Pooled Libraries, Rev A
6554
Droplet Digital PCR: Detection of DNA Methylation Application Note, Rev A
6541
Droplet Digital™ PCR: TRAPing Telomerase Activity Using Droplet Digital PCR Application Note, Rev A
6451
Droplet Digital PCR: Guidelines for Multiplexing Using Bio-Rad’s QX100 Droplet Digital PCR System Application Note, Rev A
6497
Droplet Digital PCR: Linking Microbial Physiology and Phylogeny Using Bio-Rad’s QX100 Droplet Digital PCR System Application Note, Rev A
6475
Droplet Digital PCR: High-Resolution Copy Number Variation Analysis Application Note, Rev A
[ Add to Cart (Free) ]
6424
Droplet Digital™ PCR: Ultra-Sensitive Rare Event Detection Using Bio-Rad’s QX100™ Droplet Digital PCR System Application Note, Rev B
[ Add to Cart (Free) ]
6423
Droplet Digital PCR: Ultra-Sensitive Validation Technology for RNA-Seq Application Note, Rev A
[ Add to Cart (Free) ]
6317
Detection of rare mutations in blood samples by Droplet Digital PCR (ddPCR). (Poster), Rev A
6316
Droplet Digital RT-ddPCR: Ultra-high sensitivity validation technology for RNA-Seq. (Poster), Rev A
6315
Droplet Digital PCR: 3rd generation PCR provides improved detection, quantification, identification, and characterization of microbial targets. (Poster), Rev A
6314
Droplet Digital PCR detection of HER2 expression in FFPE breast tissue samples (Poster), Rev A
6277
Probing Copy Number Variations Using Bio-Rad's QX100 Droplet Digital PCR System, Rev A
6578
Droplet Digital PCR: Multiplex Detection of KRAS Mutations in Formalin-Fixed, Paraffin-Embedded Colorectal Cancer Samples Application Note
[ Add to Cart (Free) ]
6679
Droplet Digital PCR: Multiplex Screening of KRAS Mutations in Cell-Free DNA Colorectal Cancer Samples Application Note, Ver B
6681
Discriminating Copy Number Variation from Heterogeneous Samples Using the S3™ Cell Sorter and the QX200™ Droplet Digital™ PCR System Application Note
10026868
ddPCR™ Supermix for Probes (No dUTP) Product Insert
10026235
ddPCR™ Supermix for Probes Product Insert
10028376
QX200™ ddPCR™ EvaGreen Supermix Product Insert
10028782
One-Step RT-ddPCR Kit for Probes Product Insert, Rev B
10031986
ddPCR Library Quantification Kit for Illumina TruSeq Product Insert, Rev C
10033630
ddPCR Library Quantification Kit for Ion Torrent Product Insert, Rev B
10040718
Droplet Generation Oil for Probes Product Insert
10040717
QX200 Droplet Generation Oil for EvaGreen Product Insert, Rev C
6628
Rare Mutation Detection Best Practices Guidelines
10048730
PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A
10048259
ddPCR Supermix for Residual DNA Quantification Product Insert
6712
Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital™ PCR Application Note, Ver B
7204
Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies, Ver A