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PX1 PCR Plate Sealer

PX1 PCR Plate Sealer

The PX1™ PCR Plate Sealer efficiently seals microplates used in thermal cycling. This heat sealing method helps deliver reliable data by minimizing sample evaporation during PCR amplification.

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Overview

The PX1 PCR plate sealer provides consistent and uniform sealing across an entire microplate. This semiautomated heat sealer is ready to seal in less than 3 minutes. The PX1 sealer removes human variability from plate sealing and minimizes sample evaporation from the PCR workflow. This versatile instrument is compatible with a variety of heat-sealing films and foils and a wide range of PCR plates.

Key Features and Benefits

  • Fast startup time — avoid delaying an experiment while the sealer warms up
  • Extremely intuitive — save time programming the instrument
  • Small footprint — accommodates crowded laboratory benches
  • Quickly access sealing protocols — save time by easily using stored protocols

An Intuitive User Interface

The PX1 PCR plate sealer features an intuitive touch-screen user interface. The thermal sealing process is simplified by allowing sealing temperature and time to be modified with the touch of a button.

A
PX1 User Interface example A - a button-driven graphical interface

B
PX1 User Interface example B - customizable sealing function

The intuitive touch screen makes it easy to program the PX1 PCR plate sealer. A, high-resolution touch screen presents a button-driven graphical interface to help personalize heat sealing conditions and easily view instrument status; B, the customizable sealing function saves up to 12 unique sealing protocols, eliminating the need to recall optimized sealing conditions.

Heat Sealing Films and Foils

  Optically Clear Heat Seal thumbnail image
Optically Clear
Heat Seal		 Permanent Clear Heat Seal thumbnail image
Permanent Clear
Heat Seal		 Pierceable Foil Heat Seal thumbnail image
Pierceable Foil
Heat Seal		 Peelable Foil Heat Seal thumbnail image
Peelable Foil
Heat Seal
Catalog number 1814030 1814035 1814040 1814045
Applications – Real-time PCR
– PCR		 – Water bath cycling
– PCR		 – QX100™ Droplet Digital™ PCR System
– PCR		 – Sample storage
– PCR
Maximum temperature 110°C 110°C 110°C 110°C
Minimum temperature –80°C –80°C –20°C –200°C
Peelable seal + – + +
Pierceable seal – – + –
DNase and RNase free + + + +
Sealing compatibility PP, COC, PE,* PS PP PP, PS* PP, COC*
COC, cyclic olefin copolymer; PE, polyethylene; PP, polypropylene; PS, polystyrene.
* Limited and variable compatibility based on plate composition.

 

Amplification Reagents and Plastics Brochure

Download the Amplification Reagents and Plastics Brochure for more information on Bio-Rad products for gene expression analysis:
  • Product performance data
  • Quick and easy product selection tables
  • Ordering information

Use the links below to jump to particular guide sections:


Cell Lysis icon
Cell Lysis
RNA Isolation icon
RNA Isolation
Reverse Transcription icon
Reverse Transcription
Real-Time PCR Reagents icon
Real-Time PCR Reagents

PCR Plastic Consumables icon
PCR Plastic Consumables
PrimePCR Assays and Panels icon
PrimePCR™ Assays
and Panels
PCR Run and Data Analysis icon
PCR Run and Data Analysis		  

 

FAQs

Use the Heat Sealing Films and Foils table to determine the seal that best suits your experimental needs.

 

Optimal sealing parameters vary according to the heat films and microplates used. We recommend sealing an empty plate using the guidelines found in Suggested Heat Sealing Parameters. After sealing, examine the entire plate for effective sealing and optimize sealing conditions if necessary.

Sealing temperature and/or sealing time may be increased to help improve sealing performance. Use an empty plate to optimize seal conditions. See the instruction manual for additional details about seal optimization.

The sealing frame helps secure the heat seal in place and prevents the film from moving during sealing. The sealing frame must be used for the permanent clear heat seal and the peelable foil heat seal.

The PX1 heated platen was designed with an easy to clean, nonstick surface. See the PX1™ PCR Plate Sealer Instruction Manual for directions on cleaning the heated platen. Ensure the PX1 sealer cools for at least 1 hour before cleaning.

Yes. The PX1 PCR plate sealer was designed for reliability and safety. The instrument has an adjustable auto-off feature that automatically shuts the heater off when not in use for a designated amount of time. Simply touch the screen and the instrument will automatically resume heating.

A plate sealed with a peelable film may be resealed after peeling an existing film. However, resealing a plate for subsequent use in real-time PCR is not recommended. A plate sealed with a pierceable foil heat seal may also be resealed by sealing another pierceable foil heat seal directly on top. Note: only a single seal can be used in the QX200™ Droplet Digital™ PCR system workflow. Sealing parameters may need to be adjusted.

The heated platen cools passively. Opening the PX1 PCR plate sealer drawer can help accelerate the cooling process.

No. Our heat sealing films feature a special hot melt adhesive that effectively seals microplates when used with the PX1 sealer. The temperature and pressure delivered by the PX1 will not successfully seal a microplate when traditional adhesive and pressure-sensitive seals are used.

 

Related Products

Specifications

Heat up time, min
<3
Display, cm (in)
10.9 (4.3) color touch screen
Temperature range, °C
100–190, off
Time range, sec
0.5–10.0
Dimensions (W x D x H), cm (in)
19.2 x 24.8 x 21.9 (7.6 x 9.8 x 8.6)
Weight, kg (lb)
7.3 (16)
Power, watts
840
Voltage
100–240 VAC, 50–60 Hz

Ordering

items

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(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

PCR Plates

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear
HSP9601

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, high profile, semi skirted, clear/clear
HSS9601
Pkg of 25, clear shell/clear well PCR plate to fit most instruments, rigid 2-component design
(Discontinued) Hard-Shell 96-Well PCR Plates, low profile, clear/clear, barcoded
HSR9901
Pkg of 25, clear shell/clear well PCR plate for Roche LightCycler 480 Real-Time PCR System, barcoded, rigid 2-component design
(Discontinued) Hard-Shell® 384-Well PCR Plates, thin wall, skirted, clear/clear
HSP3801
Pkg of 50, clear shell/clear well PCR plate, rigid 2-component design
(Discontinued) Hard-Shell® 384-Well PCR Plates, clear/clear, barcoded
HSR4801
Pkg of 50, clear shell/clear well PCR plate for Roche LightCycler 480 Real-Time PCR System, barcoded, rigid 2-component design
(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear
MLL9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples
(Discontinued) Multiplate™ 96-Well PCR Plates, high profile, unskirted, clear
MLP9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples

Heat Seals

(Discontinued) PCR Plate Heat Seal, clear, optical
1814030

Pkg of 100, optically clear seals for PCR and real-time PCR applications, for use with PX1 PCR Plate Sealer

(Discontinued) PCR Plate Heat Seal, clear, permanent
1814035

Pkg of 100, nonpeelable, nonpierceable clear seals for PCR, water bath cycling, and sample disposal, for use with PX1 PCR Plate Sealer

(Discontinued) PCR Plate Heat Seal, foil, pierceable
1814040

Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

(Discontinued) PCR Plate Heat Seal, foil, peelable
1814045

Pkg of 100, laminate/foil peelable seals for PCR and sample storage, for use with PX1 PCR Plate Sealer

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