PrimePCR™ PCR Primers, Assays, and Arrays

Real-time PCR primer assays consist of unlabeled PCR primer pairs for use with dye-based chemistry such as SYBR Green or EvaGreen. Probe assays for real-time PCR and Droplet Digital PCR include PCR primers and a dual-labeled fluorescent probe with your choice of fluorophore.
 

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ddPCR Assay Badge

Find Droplet Digital PCR (ddPCR) Assays

Simply enter your target of interest or sequence and order a ddPCR Assay for Gene Expression, Mutation Detection Copy Number Determination, or Genome Edit Detection.

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Find a qPCR Assay or Template

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      Organism (select one or more)

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        PrimePCR Lookup Tool

        PrimePCR Lookup Tool

        Use the PrimePCR lookup tool to find assays and arrays for your genes of interest.

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        Create a Custom PCR Plate

        Create a Custom PCR Assay

        Design a custom 96-well or 384-well PCR plate.

        Configure New Plate

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        Find a PrimePCR Array
        Find a Pathway Plate

        Find a predesigned PCR plate for your biological pathway or disease of interest.

        Order custom PCR primer or probe assays
        Customize PCR Primers or Probe Assays

        Use this option if you already have your primer/probe sequences.

        Droplet Digital™ PCR Assays

        Pricing for PrimePCR Droplet Digital PCR assays is based on assay type and the number of reactions.

        Discount applies to bulk orders of the same catalog number and is taken off the list price.

        Bulk discounts are shown below; download the pricing flyer for full details.

         

        Description

        Description

        PrimePCR Assay Selection Guide

         

        Real-Time PCR Assays

        Droplet Digital PCR Assays

        Organisms Human, mouse, rat, yeast, rhesus monkey, arabidopsis, dog, chicken, zebrafish, cow, pig, rabbit Human, mouse, rat
        Assay Chemistry Dye-based and probe assays
        Applications Gene expression
        Preamplification
        Copy number variation
        Mutation detection
        Gene expression
        Formats Individual assays
        96- and 384-well predesigned plates*
        96- and 384-well custom plates*
        Individual assays

        * Probe assays are not available in plates

        PCR Primer and Probe Assays for Real-Time PCR

        Our real-time PCR primers were designed in collaboration with leading experts in real-time PCR research. Every PCR primer pair has been experimentally validated to ensure optimal assay performance. For more information on the validation of the DNA primer pairs, see Bulletin 6262, PrimePCR Assay Validation.

        Our fluorescently labeled oligonucleotide probes were designed to enable multiplexing. These 5' nuclease probes were designed for maximum specificity and transcript coverage. qPCR probe assays are available with a FAM, HEX, TEX615, Cy5, or Cy5.5 fluorophore.

        Real-time PCR assays are available in the following formats:

        • Predesigned primer and probe assays — gene expression assays for the human and mouse genomes
        • Custom oligonucleotides — design your own PCR primer or probe assay
        • Customizable 96- and 384-well platesuse the custom plate configurator to conveniently design a PCR plate
        • Predesigned PCR Arrays — select from a wide variety of disease- and pathway-specific arrays
        • PreAmp assays — 1,000-fold unbiased target-specific preamplification of limited nucleic acid

        Controls and Reference Assays are available to facilitate assay normalization and control:

        • Experimental Controls — for evaluating reverse transcription performance, RNA quality, genomic DNA contamination, and PCR reaction performance
        • Reference Gene Assays — for relative gene expression analysis to normalize for variation in the amount of input messenger RNA (mRNA) between samples
        • DNA templates — serve as a positive real-time PCR control for the corresponding gene assay

        All real-time PCR assays and arrays can be used with CFX real-time PCR detection systems and data analysis software, streamlining PCR data collection and analysis.

        Probe Assays for Droplet Digital PCR

        Droplet Digital PCR (ddPCR™) technology provides an absolute measure of target DNA molecules with unrivaled accuracy, precision, and sensitivity, accelerating discovery and enabling new research strategies for inherited disorders, cancer, and infectious disease. Probe assays for the QX200™ Droplet Digital PCR system enable the fine quantitative discrimination required to resolve small fold changes without a standard curve. ddPCR probe assays are available with a FAM or HEX fluorophore.

        For more information, see Bulletin 6512.

        ddPCR assays are available in the following formats:

        • Predesigned mutation detection probe assays — for human samples
        • Predesigned copy number variation (CNV) probe assays — for human samples
        • Custom oligonucleotides — design your own ddPCR probe assay

        PrimePCR assays, plates, and controls have a guaranteed shelf life of 12 months when stored at 4°C.

        PrimePCR™ Analysis Software

        PrimePCR™ Analysis Software

        Our custom data analysis software enables gene expression analysis of PrimePCR data generated on any real-time PCR instrument. Analysis options include bar chart, clustergram, scatter plot, volcano plot, and heat map analysis.

        Go to Download

        Download

        Download

        PrimePCR™ Analysis Software
        GeneStudy_1.0.030.1023
        Data analysis software that enables gene expression analysis of PrimePCR data generated on any real-time PCR instrument. Analysis options include bar chart, clustergram, scatter plot, volcano plot, or heat map analysis.

        Documents

        Documents

        Number Description Options
        10000088666
        Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G
        10042030
        PrimePCR™ PreAmp Assay Quick Guide, Rev A
        10039761
        PrimePCR™ Assays Quick Guide, Ver B
        6763
        Gene Expression Real-Time PCR Workflow Flier
        [ Add to Cart (Free) ]
        6290
        PrimePCR Assays and Panels Brochure, Rev D
        6595
        PrimePCR™ Assays and Panels for Real-Time PCR Brochure, Rev A
        [ Add to Cart (Free) ]
        6512
        PrimePCR Assays for Droplet Digital PCR Brochure, Rev A
        [ Add to Cart (Free) ]
        6629
        High-Throughput Real-Time PCR Solutions Brochure
        10041702
        PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A
        10048730
        PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A
        10033173
        ddPCR Copy Number Variation Assays, Validated, Product Insert
        10033487
        ddPCR Mutation Detection Assays, Validated, Product Insert
        10047489
        ddPCR Mutation Detection Assays Product Insert
        6262
        PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B
        6263
        PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B
        6679
        Droplet Digital PCR: Multiplex Screening of KRAS Mutations in Cell-Free DNA Colorectal Cancer Samples Application Note, Ver B
        6634
        A Fast Semi-Automated Quantitative PCR Method for Monitoring Differential Gene Expression for Drug Target Discovery, Ver B
        6734
        PrimePCR™ Publications List, Rev A
        6777
        Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR
        6816
        Protocol for Multiplex RT-qPCR Analysis Using PrimePCR™ Assays, Ver A
        10000065278
        ddPCR™ NHEJ Genome Edit Detection Assays, Ver A
        10000065279
        ddPCR™ HDR Genome Edit Detection Assays, Predesigned, Ver A
        10000065281
        ddPCR™ HDR Genome Edit Detection Assays, Ver A

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