PTC Tempo Thermal Cycler

Choose the PTC Tempo Thermal Cycler, which offers the thermal accuracy and uniformity you can trust to get the consistent performance you need in PCR.

Ordering Information

Consult with a Specialist

Overview

New Version Available!

PTC Tempo Software 3.0.0.8 is now available for all PTC Tempo models. This update incorporates all the PTC Tempo Thermal Cycler models, connectivity to Bio-Rad’s BR.io app to manage PCR protocol files and view run status, and the ability to integrate with laboratory automation systems using the PTC Tempo Automation API. Go to bio-rad.com/PCRUpdates to upgrade your PTC Tempo thermal cycler's software and firmware. Follow directions within the 10000160470 Release Notes to upgrade your PTC Tempo today.

The PTC Tempo Thermal Cycler represents the next generation of conventional PCR thermal cyclers from Bio-Rad. Engineered to maintain thermal performance that delivers accurate results, the PTC Tempo Thermal Cycler offers the flexibility to scale with your laboratory. The new design features a refreshed, intuitive user interface and flexible connectivity options for streamlining protocol management. A motorized lid enables automation, taking your PCR to the next level with modern instrument control.

The PTC Tempo Thermal Cycler is available in multiple fixed-block configurations: PTC Tempo 96 Thermal Cycler for most general PCR applications and PTC Tempo Deepwell Thermal Cycler with larger volumes up to 125 µl.

Features and Benefits

Programmable thermal gradient — for optimization of annealing temperatures
Large touch screen — for streamlined protocol programming and editing
Heated lid — for use with various vessels and sealer types
Expanded connectivity — Wi-Fi, ethernet, and USB
Automated lid — for integration with automation robotics
Cloud connectivity — create and send protocols from your BR.io cloud platform
Network storage drive access — for excellent data management

Applications and Uses

Amplification/PCR
Cloning
Cycle sequencing
Gene expression studies
Mutagenesis

More Information

Related Categories

Related Technologies

Specifications

Sample block type

Fixed

Weight

15 kg (33 lb)

Dimensions (W x D x H)

28 x 50 x 26 cm (11 x 20 x 10 in)

Power

100–240 V, 50/60 Hz, 850 W max

Electrical approvals

IEC, CE

Memory

4.6 GB

Noise level

<90 dB

Lid type

Automated

Touchscreen display

8 in. LCD

Programming options

Step-based graphical and automatic

Security features for regulated environments

Password protection and secure mode

Reporting

Exportable run logs and system logs

Temperature control modes

Calculated and block

Onboard operating system

Onboard operating system Linux

Communications

Wi-Fi, BR.io, USB A 2.0, Ethernet

Heating and cooling method

Peltier

Lid heating

30–110°C

Temperature range

4–100°C

Thermal accuracy

+/- 0.2°C of programmed target at 90°C

Thermal uniformity

+/- 0.4°C well-to-well within 10 sec of arrival at 90°C

Gradient operational range

30–100°C

Gradient programmable span

8 rows, 1–24°C

Ordering

items

Use the filters below to refine results!

(Discontinued) PTC Tempo 96 Thermal Cycler

12015382

96-well, thermal cycler for PCR with cables; for research use only.

(Discontinued) PTC Tempo Deepwell Thermal Cycler

12015392

96-deepwell, thermal cycler for PCR with cables; for research use only.

(Discontinued) PTC Tempo 384 Thermal Cycler

12015394

384-well, thermal cycler for PCR with cables; for research use only.

(Discontinued) PTC Tempo 48/48 Thermal Cycler

12015309

48/48-well, thermal cycler for PCR with cables; for research use only.

Accessories

(Discontinued) PTC Tempo Thermal Cycler API, 1 License

12019776

For use with the PTC Tempo 96, PTC Tempo Deepwell, or PTC Tempo 384 Thermal Cycler. Each instrument requires 1 license to utilize the instrument API for automation or robotics integration.

(Discontinued) CFX Opus Ethernet Cable

12013205

A cat-5 ethernet cable for connecting the CFX Opus Real-Time PCR System to ethernet ports.

(Discontinued) Tube Frame

1849000

Tube frame to provide extra support for individual 0.2 ml tubes in the dual 48- and 96-well fast reaction modules

(Discontinued) Tube Frame

1849001

Tube frame to provide extra support for individual 0.2 ml tubes in the 96–deep well reaction module

(Discontinued) Tube Frame for 96-Well Reaction Module

12021890

Tube frame to provide extra support for individual 0.2 ml tubes in the 96-well reaction modules

(Discontinued) PTC Tempo Thermal Cycler Wi-Fi Adapter, US Only

12018650

Wi-Fi antenna enables Wi-Fi connection for PTC Tempo Thermal Cycler in the United States.

(Discontinued) PTC Tempo Deepwell Tube Frame for 0.5 ml Tubes

12020274

Tube frame to provide extra support for individual 0.5 ml tubes in the PTC Tempo Deepwell Thermal Cycler

(Discontinued) Plate Lifter for 384-Well Reaction Module

12014294

Plate lifter to provide support when using 384-well plates with the CFX Opus 384 or PTC Tempo 384

Kits & Reagents

(Discontinued) iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

1708890

25 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

(Discontinued) iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns

1708891

100 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

(Discontinued) iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns

1708896

25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

(Discontinued) iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns

1708897

100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

(Discontinued) iTaq™ DNA Polymerase, 250 U (5 U/µl), 50 µl

1708870

250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl₂

(Discontinued) iTaq™ DNA Polymerase, 5,000 U (5 U/µl), 1 ml

1708875

5,000 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 25 ml each 10x PCR buffer and 50 mM MgCl₂

(Discontinued) MgCl₂ Solution for PCR, 50 mM, 1.25 ml

1708872

1.25 ml, 50 mM MgCl₂ solution, for PCR applications

(Discontinued) dNTP Solution Mix, 10 mM, 200 µl

1708874

200 µl, premixed dNTP solution, 10 mM each (dATP, dCTP, dGTP, dTTP)

(Discontinued) iProof GC Master Mix, 100 x 50 µl rxns, 2.5 ml

1725320

100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates

(Discontinued) iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl

1725330

50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl₂ solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

(Discontinued) iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl

1725331

200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl₂ solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

(Discontinued) iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl

1725301

100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl₂ solution, DMSO

(Discontinued) iProof™ High-Fidelity DNA Polymerase, 500 U (2 U/µl), 250 µl

1725302

500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl₂ solution, DMSO

(Discontinued) iProof HF Master Mix, 100 x 50 µl rxns, 2.5 ml

1725310

100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates

Electrophoresis Gel Systems

(Discontinued) Sub-Cel GT Horizontal Electrophoresis System, 15 x 15 cm tray, with gel caster and casting gates

1704482

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 15 cm (W x L) UV-transparent tray, casting gates, gel caster

(Discontinued) Sub-Cell GT Horizontal Electrophoresis System, 15 x 25 cm tray, with gel caster

1704484

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 25 cm (W x L) UV-transparent tray, gel caster

(Discontinued) Wide Mini-Sub Cell GT Horizontal Electrophoresis System, 15 x 10 cm tray

1704468

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm (W x L) UV-transparent tray

(Discontinued) Wide Mini-Sub Cell GT Horizontal Electrophoresis System, 15 x 10 cm tray, with gel caster

1704469

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm (W x L) UV-transparent tray, mini-gel caster

(Discontinued) Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray

1704466

Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray

(Discontinued) Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray, with mini-gel caster

1704467

Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm (W x L) UV-transparent tray, mini-gel caster

Plastics

(Discontinued) Microseal® 'F' PCR Plate Seal, foil, pierceable

MSF1001

Pkg of 100, aluminized foil seal for PCR plates, pierceable. Note: This item is now available under the new material number MSF1001B. For future orders please proceed here.