Biologics Analysis Workflow

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Overview

The Biologics Analysis Workflow is a four-step solution for biological product analysis. This integrated system enables expedited electrophoresis, simplified staining, easy analysis, and data reproducibility.
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Description

Description

The Biologics Analysis Workflow is a suite of products designed and validated to assess the purity or identity of biological products in a cGMP regulatory environment. The Biologics Analysis Workflow provides:

  • Increased throughput
  • Simplified, sensitive gel staining
  • Accurate and reproducible imaging
  • Easy data analysis

The Biologics Analysis Workflow includes a set of integrated protein electrophoresis solutions for each step of the workflow:

Step 1: Separate samples

Criterion TGX precast gels — increased throughput with fast run times and more sample loading wells

The unique properties of TGX chemistry reduce run times to as little as 20 minutes. These gels have a 1-year shelf life and utilize the standard Laemmli buffer system. The midi-sized Criterion format allows you to load up to 26 samples while maintaining the required resolution. Additionally, the characteristic upper buffer chamber reduces the amount of running buffer required.

Step 2: Stain proteins

QC Colloidal Coomassie stain — highly sensitive, reliable, end-point stain designed for quantitative analysis of proteins

QC Colloidal Coomassie stain enables the detection of nanogram levels of protein. The colloidal chemistry improves the protein-to-dye binding ratio, reducing background staining and improving sensitivity. QC Colloidal Coomassie stain is an end-point stain that was designed and optimized for quantitative analysis with the GS-900 densitometer. The stain is ready to use without mixing or the addition of other reagents. The stain is formulated with ethanol instead of methanol, eliminating the need for hazardous waste disposal.

Step 3: Visualize proteins

GS-900 calibrated densitometer — reproducible imaging of protein gels, blots, and film across a wide dynamic range

To ensure the accuracy of each scan, the GS-900 calibrated densitometer contains internal optical density tablets that are scanned and used for calibration before each run. The calibration of the GS-900 densitometer can be validated with the GS-900 calibrated densitometer installation and qualification kit, which utilizes an external target to confirm accuracy of the internal target, ensuring the accuracy and reproducibility of the GS-900 densitometer. With red/green/blue tricolor CCD technology, the GS-900 calibrated densitometer enables the imaging and quantitation of film, blots, and gels stained with a wide variety of stains. The wide dynamic range permits the quantitation of proteins from 0–3.4 OD.

Step 4: Analyze results

Image Lab image analysis software — effortless analysis of samples with an intuitive, user-friendly interface

Gel analysis is greatly simplified with Image Lab software. The ability to automate analyses saves time and ensures consistent analysis from run to run. Image Lab software includes detailed tutorials and requires no previous imaging experience to produce optimum gel and blot images. The flexible system allows users to easily modify analysis settings to match SOPs and fine-tune band detection, background level, and other settings. Image Lab software makes reporting easy with the ability to create customized reports and meets regulatory requirements with an optional 21 CFR Part 11 module.

Specifications

Specifications

Calibration frequency
Automatic calibration before every scan
21 CRF Part 11
Yes (optional software package)
Acquisition software
ImageLab software ≥ 5.0
Light source
LED
Wavelength
400–750 nm
Absorbance range
0.0 to 3.4 OD Transmissive
0.0 to 2.7 OD RD reflective
Transmissive scan area
29 × 33 cm
Reflective scan area
29 × 33 cm
Bit depth
16-bit grayscale
Minimum pixel size
36.3 µm (700 dpi)
Image Lab™ software OS compatibility
Windows XP, 7
Connection type
USB 2.0
Imager dimensions (W x L x H)
62.7 × 37.6 × 18.5 cm
Imager weight
16.1 kg
Applications
Densitometry
Calibrated colorimetric gel and blot quantitation

Ordering

Ordering

items

Use the filters below to refine results!

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1707993
Pkg of 1, set of protocols for validating the calibration of GS-900 calibrated densitometer (#170-7991), Image Lab Security Edition with 21 CFR Part 11 Module
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1707994
Pkg of 1, set of protocols and one NIST-traceable external step table for validating the calibration of GS-900™ calibrated densitometer (#170-7991)

Kits & Reagents

Kits & Reagents

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1610803
1 L, ready-to-use, non-hazardous colloidal Coomassie G-250 stain for protein polyacrylamide gels
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1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE
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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)
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(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent

Precast Gels

Precast Gels

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5671023
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671024
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671025
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671033
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671034
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671035
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671083
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671084
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671085
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671081
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671082
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671093
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671094
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671091
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671092
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671103
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671104
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671102
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671112
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671123
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671124
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671125
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671121
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671122
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

MW Standards

MW Standards

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1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
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1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
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1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
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1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
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1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications
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1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
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1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
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1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Documents

Documents

Number Description Options
6385
Biologics Analysis Workflow™ Brochure, Rev A
[ Add to Cart (Free) ]
10031959
Instruction Manual, Image Lab Software for the GS-900 Calibrated Densitometer, Rev B
10033507
Biologics Analysis Workflow Product Information Sheet
6387
Biologics Analysis Workflow™ Model Comparability Study — Obtaining cGMP Approval for Validated SDS-PAGE Assays with the GS-900™ Calibrated Densitometer
[ Add to Cart (Free) ]
6040
Electrophoresis Guide, Interactive PDF, Rev C
4006028
Instruction Manual, Laemmli Sample Buffer, Rev J
10032602
QC Colloidal Coomassie Stain Manual, Rev A
6421
Image Lab™ Software for the GS-900™ Densitometer Quick Start Guide, Rev A
10030885
Installing your GS-900™ Calibrated Densitometer Quick Start Guide, Rev C