Biologics Analysis Workflow

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Biologics Analysis Workflow

Overview

Biologics Analysis Workflow

Biologics Analysis Workflow

Integrated Protein Electrophoresis Solutions

Faster, easier, and more accurate analysis tools optimized for cGMP labs

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The Biologics Analysis Workflow is a four-step solution for biological product analysis. This integrated system enables expedited electrophoresis, simplified staining, easy analysis, and data reproducibility.
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Ordering Information


  • Description
  • Specifications
  • Ordering
  • Kits & Reagents
  • Precast Gels
  • MW Standards
  • Documents

Description

The Biologics Analysis Workflow is a suite of products designed and validated to assess the purity or identity of biological products in a cGMP regulatory environment. The Biologics Analysis Workflow provides:

  • Increased throughput
  • Simplified, sensitive gel staining
  • Accurate and reproducible imaging
  • Easy data analysis

The Biologics Analysis Workflow includes a set of integrated protein electrophoresis solutions for each step of the workflow:

Step 1: Separate samples

Criterion TGX precast gels — increased throughput with fast run times and more sample loading wells

The unique properties of TGX chemistry reduce run times to as little as 20 minutes. These gels have a 1-year shelf life and utilize the standard Laemmli buffer system. The midi-sized Criterion format allows you to load up to 26 samples while maintaining the required resolution. Additionally, the characteristic upper buffer chamber reduces the amount of running buffer required.

Step 2: Stain proteins

QC Colloidal Coomassie stain — highly sensitive, reliable, end-point stain designed for quantitative analysis of proteins

QC Colloidal Coomassie stain enables the detection of nanogram levels of protein. The colloidal chemistry improves the protein-to-dye binding ratio, reducing background staining and improving sensitivity. QC Colloidal Coomassie stain is an end-point stain that was designed and optimized for quantitative analysis with the GS-900 densitometer. The stain is ready to use without mixing or the addition of other reagents. The stain is formulated with ethanol instead of methanol, eliminating the need for hazardous waste disposal.

Step 3: Visualize proteins

GS-900 calibrated densitometer — reproducible imaging of protein gels, blots, and film across a wide dynamic range

To ensure the accuracy of each scan, the GS-900 calibrated densitometer contains internal optical density tablets that are scanned and used for calibration before each run. The calibration of the GS-900 densitometer can be validated with the GS-900 calibrated densitometer installation and qualification kit, which utilizes an external target to confirm accuracy of the internal target, ensuring the accuracy and reproducibility of the GS-900 densitometer. With red/green/blue tricolor CCD technology, the GS-900 calibrated densitometer enables the imaging and quantitation of film, blots, and gels stained with a wide variety of stains. The wide dynamic range permits the quantitation of proteins from 0–3.4 OD.

Step 4: Analyze results

Image Lab image analysis software — effortless analysis of samples with an intuitive, user-friendly interface

Gel analysis is greatly simplified with Image Lab software. The ability to automate analyses saves time and ensures consistent analysis from run to run. Image Lab software includes detailed tutorials and requires no previous imaging experience to produce optimum gel and blot images. The flexible system allows users to easily modify analysis settings to match SOPs and fine-tune band detection, background level, and other settings. Image Lab software makes reporting easy with the ability to create customized reports and meets regulatory requirements with an optional 21 CFR Part 11 module.


Specifications

Calibration frequency Automatic calibration before every scan
21 CRF Part 11 Yes (optional software package)
Acquisition software ImageLab software ≥ 5.0
Light source LED
Wavelength 400–750 nm
Absorbance range 0.0 to 3.4 OD Transmissive
0.0 to 2.7 OD RD reflective
Transmissive scan area 29 × 33 cm
Reflective scan area 29 × 33 cm
Bit depth 16-bit grayscale
Minimum pixel size 36.3 µm (700 dpi)
Image Lab™ software OS compatibility Windows XP, 7
Connection type USB 2.0
Imager dimensions (W x L x H) 62.7 × 37.6 × 18.5 cm
Imager weight 16.1 kg
Applications Densitometry
Calibrated colorimetric gel and blot quantitation

Ordering

1689 4–15% 4–20% 7.5% 8–16% 10% 10–20% 12% 18% Any kD 2929 12+2 (45 µl) 18 (30 µl) 26 (15 µl) IPG/prep (550 µl) Prep+2 (700 µl)
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GS-900 Calibrated Densitometer Regulatory Tools Package

1707993
Pkg of 1, set of protocols for validating the calibration of GS-900 calibrated densitometer (#170-7991), Image Lab Security Edition with 21 CFR Part 11 Module

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$9,928.00 
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GS-900™ Installation Qualification/Operations Qualification (IQ/OQ) Kit

1707994
Pkg of 1, set of protocols and one NIST-traceable external step table for validating the calibration of GS-900™ calibrated densitometer (#170-7991)

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$6,195.00 
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Kits & Reagents

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QC Colloidal Coomassie Stain

1610803
1 L, ready-to-use, non-hazardous colloidal Coomassie G-250 stain for protein polyacrylamide gels

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$100.00 
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Hazardous  
4x Laemmli Sample Buffer

1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE

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$15.00 
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2x Laemmli Sample Buffer

1610737
30 ml, premixed protein sample buffer for SDS-PAGE

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$14.00 
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10x Tris/Glycine/SDS

1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$32.00 
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2-Mercaptoethanol

1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

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$27.00 
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Dithiothreitol (DTT)

1610611
5 g, dithiothreitol (DTT) reducing agent

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$94.00 
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Precast Gels

21 items

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7.5% Criterion™ TGX™ Precast Midi Precast Midi Protein Gel, 12+2 well, 45 µl

5671023
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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7.5% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671024
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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7.5% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671025
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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10% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671033
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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10% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671034
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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10% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671035
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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12% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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12% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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12% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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18% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671083
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671084
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671085
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671081
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–15% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl

5671082
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671093
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671094
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671091
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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4–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl

5671092
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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8–16% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671103
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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8–16% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671104
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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8–16% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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8–16% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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8–16% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl

5671102
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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10–20% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671121)
(Discontinued) 10–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl

5671112
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671122)
AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671123
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671124
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671125
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671121
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl

5671122
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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MW Standards

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
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Precision Plus Protein™ All Blue Prestained Protein Standards

1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications

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$111.00 
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Precision Plus Protein™ All Blue Prestained Protein Standards, value pack

1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications

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$495.00 
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Precision Plus Protein Dual Color Standards, 500 µl

1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications

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$146.00 
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Precision Plus Protein™ Dual Color Standards, 2.5 ml

1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications

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$596.00 
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Precision Plus Protein™ Dual Xtra Prestained Protein Standards

1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications

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$157.00 
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Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards

1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications

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$157.00 
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Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack

1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

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$642.00 
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Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml

1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

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$105.00 
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Precision Plus Protein™ Unstained Protein Standards, Strep-tagged, recombinant, 5 ml

1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

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$461.00 
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Documents

Number Description Options
6385 Biologics Analysis Workflow™ Brochure, Rev A Click to download [ Add to Cart (Free) ]
10031959 Instruction Manual, Image Lab Software for the GS-900 Calibrated Densitometer, Rev B Click to download
10033507 Biologics Analysis Workflow Product Information Sheet Click to download
6387 Biologics Analysis Workflow™ Model Comparability Study — Obtaining cGMP Approval for Validated SDS-PAGE Assays with the GS-900™ Calibrated Densitometer Click to download [ Add to Cart (Free) ]
6040 Electrophoresis Guide, Interactive PDF, Rev C Click to download
4006028 Instruction Manual, Laemmli Sample Buffer, Rev J Click to download
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