Description
Description
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What is the Biologics Analysis Workflow?
The GS-900 Calibrated Densitometer is designed to reproducibly image gels, blots, and film and quantitate proteins across a wide dynamic range. The GS-900 Densitometry System uses transmissive and reflective imaging to scan chromogenic samples at the optimal detection wavelength. The three-color red, green, and blue CCD imaging technology enables accurate, reproducible quantitation with a wide variety of common stains.
To meet cGMP environment needs, the GS-900 Densitometer offers a security edition software module for 21 CFR 11 complliance and an Installation and Operations Qualification (IQ/OQ) Kit.
Applications and Uses
- Imaging of diverse samples on 1-D and 2-D gels, colorimetric dot and slot blots, film-based chemiluminescent blots, and autoradiograms
- Protein quantitation for Coomassie Blue- and silver-stained gels over a wide dynamic range of 0–3.4 OD
- Scanning large gels — oversized 29 × 33 cm imaging area
- Rapid image acquisition and analysis with intuitive, user-friendly Image Lab Software
Features and Benefits of the GS-900 Calibrated Densitometer
- Automatic self-calibration before each run
- The GS-900 Calibrated Densitometer uses built-in NIST-traceable step tablets for internal calibration to ensure the accuracy of each scan
- Internal calibration can be validated with an external NIST-traceable target to confirm accuracy and reproducibility using the GS-900 Calibrated Densitometer Installation and Operations Qualification Kit (1707994)
- One-touch operation button for further ease of image acquisition
- Image Lab Software greatly simplifies image analysis
- Automated analysis saves time and ensures consistency between runs
- Includes detailed tutorials; no previous imaging experience required to produce optimum gel and blot images
- Flexible analysis — simply modify user settings to match SOPs and fine-tune band detection, background level, and other settings
- Easy reporting with the ability to create customized reports
- Meets 21 CFR Part 11 regulatory requirements with an optional Image Lab Software Security Module (1709691)
- 16-bit precision and 36.3 μm resolution for the analysis of very close bands on a gel
- Sealed imaging area to accommodate wet samples of variable thickness
Related Products
The GS-900 Calibrated Densitometry System and Image Lab Software are part of the Biologics Analysis Workflow — an integrated suite of products designed and validated to assess the purity or identity of biological products in a cGMP environment.
The Biologics Analysis Workflow provides:
- Increased electrophoresis throughput
- Simplified, sensitive, and reproducible gel staining
- Accurate and reproducible imaging
- Easy data analysis and report generation
Specifications
Specifications
Calibration frequency
Automatic calibration before every scan
21 CRF Part 11
Yes (optional software package)
Acquisition software
ImageLab™ software ≥5.0
Light source
LED
Wavelength
400–750 nm
Absorbance range
0.0 to 3.4 OD Transmissive
0.0 to 2.7 OD RD reflective
0.0 to 2.7 OD RD reflective
Transmissive scan area
29 × 33 cm
Reflective scan area
29 × 33 cm
Bit depth
16-bit grayscale
Minimum pixel size
36.3 µm (700 dpi)
Image Lab™ software OS compatibility
Windows XP, 7, and 10
Connection type
USB 2.0
Imager dimensions (W x L x H)
62.7 × 37.6 × 18.5 cm
Imager weight
16.1 kg
Applications
Densitometry
Calibrated colorimetric gel and blot quantitation
Calibrated colorimetric gel and blot quantitation
Ordering
Ordering
items
Use the filters below to refine results!
Image
(Discontinued) GS900 Calibrated Densitometer
1707989
Gel densitometry system, includes scanner and cables, optional PC-compatible Image Lab™ software, 21 CFR part 11 and installation Qualification/Operations Module.
Image
1707991
Biologics Analysis Workflow product suite, includes gel densitometry system, scanner, cables, PC-compatible Image Lab™ Software, optional 21 CFR Part 11 and Instrument Qualification/Operations Qualification module
Image
(Discontinued) GS-900 Calibrated Densitometer Regulatory Tools Package
1707993
Pkg of 1, set of protocols for validating the calibration of GS-900 calibrated densitometer (#170-7991), Image Lab Security Edition with 21 CFR Part 11 Module
Image
1707994
Pkg of 1, set of protocols and one NIST-traceable external step table for validating the calibration of GS-900™ calibrated densitometer (#170-7991)
Image
(Discontinued) Image Lab™ Software
1709690
Software, PC or Mac, for automated image capture, optimization, and 1-D data analysis, for use with Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ XRS+, ChemiDoc MP, and GS-900™ imaging systems
Image
(Discontinued) Image Lab Software, Security Edition, 1 license
1709691
FDA CFR 21 Part 11 module for Image Lab Software, 1 license, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems
Image
(Discontinued) Image Lab Software, Security Edition, 5 licenses
1709692
FDA CFR 21 Part 11 module for Image Lab Software, 5 licenses, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems
Image
(Discontinued) Image Lab Software, Security Edition, 10 licenses
1709693
FDA CFR 21 Part 11 module for Image Lab Software, 10 licenses, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems
Kits & Reagents
Kits & Reagents
Image
(Discontinued) QC Colloidal Coomassie Stain
1610803
1 L, ready-to-use, non-hazardous colloidal Coomassie G-250 stain for protein polyacrylamide gels
Image
(Discontinued) 4x Laemmli Sample Buffer
1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE
Image
(Discontinued) 2x Laemmli Sample Buffer
1610737
30 ml, premixed protein sample buffer for SDS-PAGE
Image
(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
Image
(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent
Image
(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)
Precast Gels
Precast Gels
Image
5671085
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
5671084
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
5671083
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
5671095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
5671094
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
5671093
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl
5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl
5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
MW Standards
MW Standards
Image
1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
Image
1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
Image
(Discontinued) Precision Plus Protein Dual Color Standards, 500 µl
1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
Image
(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml
1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
Image
1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications
Image
1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
Image
1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
Image
1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications
Image
1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
Image
1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications
Documents
Documents
Login Required Number Description Options
7250
From Discovery to Manufacturing: A Workflow for Protein-Based Immunotherapies
10030885
Installing your GS-900™ Calibrated Densitometer Quick Start Guide, Rev C
6421
Image Lab™ Software for the GS-900™ Densitometer Quick Start Guide, Rev A
10000076954
GS-900™ Calibrated Densitometer with Image Lab™ Software
6385
Biologics Analysis Workflow™ Brochure, Rev A
10033507
Biologics Analysis Workflow Product Information Sheet
4006028
Instruction Manual, Laemmli Sample Buffer, Rev J
10032602
QC Colloidal Coomassie Stain Manual, Rev A
6040
Electrophoresis Guide, Interactive PDF, Rev C
2895
Protein Blotting Guide, Ver C
2651
2-D Electrophoresis Workflow How-To Guide, Rev F
6387
Biologics Analysis Workflow™ Model Comparability Study — Obtaining cGMP Approval for Validated SDS-PAGE Assays with the GS-900™ Calibrated Densitometer