GS-900 Calibrated Densitometer

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GS-900-calibrated-densitometer-detail.jpg

Overview

The GS-900 Calibrated Densitometer is a self-calibrating 21 CFR Part 11 gel imaging system for acquiring gel, blot, and film images. The GS-900 Densitometer is an integral part of the Biologics Analysis Workflow, a complete, integrated solution.
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Description

Description

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The GS-900 Calibrated Densitometer is designed to reproducibly image gels, blots, and film and quantitate proteins across a wide dynamic range. The GS-900 Densitometry System uses transmissive and reflective imaging to scan chromogenic samples at the optimal detection wavelength. The three-color red, green, and blue CCD imaging technology enables accurate, reproducible quantitation with a wide variety of common stains.

To meet cGMP environment needs, the GS-900 Densitometer offers a security edition software module for 21 CFR 11 complliance and an Installation and Operations Qualification (IQ/OQ) Kit.

Applications and Uses

  • Imaging of diverse samples on 1-D and 2-D gels, colorimetric dot and slot blots, film-based chemiluminescent blots, and autoradiograms
  • Protein quantitation for Coomassie Blue- and silver-stained gels over a wide dynamic range of 0–3.4 OD
  • Scanning large gels — oversized 29 × 33 cm imaging area
  • Rapid image acquisition and analysis with intuitive, user-friendly Image Lab Software

Features and Benefits of the GS-900 Calibrated Densitometer

  • Automatic self-calibration before each run
    • The GS-900 Calibrated Densitometer uses built-in NIST-traceable step tablets for internal calibration to ensure the accuracy of each scan
    • Internal calibration can be validated with an external NIST-traceable target to confirm accuracy and reproducibility using the GS-900 Calibrated Densitometer Installation and Operations Qualification Kit (1707994)
    • One-touch operation button for further ease of image acquisition
  • Image Lab Software greatly simplifies image analysis
    • Automated analysis saves time and ensures consistency between runs
    • Includes detailed tutorials; no previous imaging experience required to produce optimum gel and blot images
    • Flexible analysis — simply modify user settings to match SOPs and fine-tune band detection, background level, and other settings
    • Easy reporting with the ability to create customized reports
  • Meets 21 CFR Part 11 regulatory requirements with an optional Image Lab Software Security Module (1709691)
  • 16-bit precision and 36.3 μm resolution for the analysis of very close bands on a gel
  • Sealed imaging area to accommodate wet samples of variable thickness

Related Products

The GS-900 Calibrated Densitometry System and Image Lab Software are part of the Biologics Analysis Workflow — an integrated suite of products designed and validated to assess the purity or identity of biological products in a cGMP environment.

The Biologics Analysis Workflow provides:

  • Increased electrophoresis throughput
  • Simplified, sensitive, and reproducible gel staining
  • Accurate and reproducible imaging
  • Easy data analysis and report generation

Specifications

Specifications

Calibration frequency
Automatic calibration before every scan
21 CRF Part 11
Yes (optional software package)
Acquisition software
ImageLab™ software ≥5.0
Light source
LED
Wavelength
400–750 nm
Absorbance range
0.0 to 3.4 OD Transmissive
0.0 to 2.7 OD RD reflective
Transmissive scan area
29 × 33 cm
Reflective scan area
29 × 33 cm
Bit depth
16-bit grayscale
Minimum pixel size
36.3 µm (700 dpi)
Image Lab™ software OS compatibility
Windows XP, 7, and 10
Connection type
USB 2.0
Imager dimensions (W x L x H)
62.7 × 37.6 × 18.5 cm
Imager weight
16.1 kg
Applications
Densitometry
Calibrated colorimetric gel and blot quantitation

Ordering

Ordering

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1707989
Gel densitometry system, includes scanner and cables, optional PC-compatible Image Lab™ software, 21 CFR part 11 and installation Qualification/Operations Module.
170-7991_thumb.jpg
1707991
Gel densitometry system, includes scanner, cables, PC-compatible Image Lab™ software, optional 21 CFR Part 11 and Installation Qualification/Operations Qualification Module
170-7993_thumb.jpg
1707993
Pkg of 1, set of protocols for validating the calibration of GS-900 calibrated densitometer (#170-7991), Image Lab Security Edition with 21 CFR Part 11 Module
170-7994_thumb.jpg
1707994
Pkg of 1, set of protocols and one NIST-traceable external step table for validating the calibration of GS-900™ calibrated densitometer (#170-7991)
170-9690_tn.jpg
1709690
Software, PC or Mac, for automated image capture, optimization, and 1-D data analysis, for use with Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ XRS+, ChemiDoc MP, and GS-900™ imaging systems
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1709691
FDA CFR 21 Part 11 module for Image Lab Software, 1 license, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems
170-9692_thumb.jpg
1709692
FDA CFR 21 Part 11 module for Image Lab Software, 5 licenses, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems
170-9693_thumb.jpg
1709693
FDA CFR 21 Part 11 module for Image Lab Software, 10 licenses, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems

Kits & Reagents

Kits & Reagents

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1610803
1 L, ready-to-use, non-hazardous colloidal Coomassie G-250 stain for protein polyacrylamide gels
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1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE
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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent
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(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

Precast Gels

Precast Gels

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5671085
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671084
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671083
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671094
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671093
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

MW Standards

MW Standards

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1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
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1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
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1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
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1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
161-0377_tn.jpg
1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications
161-0377_tn.jpg
1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
161-0375_tn.jpg
1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
161-0375_tn.jpg
1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Documents

Documents

Number Description Options
7250
From Discovery to Manufacturing: A Workflow for Protein-Based Immunotherapies
7051
Imaging Systems Family Flier
[ Add to Cart (Free) ]
10030885
Installing your GS-900™ Calibrated Densitometer Quick Start Guide, Rev C
6421
Image Lab™ Software for the GS-900™ Densitometer Quick Start Guide, Rev A
10000076954
GS-900™ Calibrated Densitometer with Image Lab™ Software
6385
Biologics Analysis Workflow™ Brochure, Rev A
[ Add to Cart (Free) ]
10033507
Biologics Analysis Workflow Product Information Sheet
4006028
Instruction Manual, Laemmli Sample Buffer, Rev J
10032602
QC Colloidal Coomassie Stain Manual, Rev A
6040
Electrophoresis Guide, Interactive PDF, Rev C
2895
Protein Blotting Guide, Ver C
2651
2-D Electrophoresis Workflow How-To Guide, Rev F
6387
Biologics Analysis Workflow™ Model Comparability Study — Obtaining cGMP Approval for Validated SDS-PAGE Assays with the GS-900™ Calibrated Densitometer
[ Add to Cart (Free) ]