GS-900 Calibrated Densitometer

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GS-900 Calibrated Densitometer

Overview

Model Comparability Study

Biologics Analysis Workflow
Model Comparability Study

Obtaining cGMP Approval for Validated SDS-PAGE Assays with the GS-900™ Calibrated Densitometer

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Biologics Analysis Workflow

Biologics Analysis Workflow

Integrated Protein Electrophoresis Solutions

Faster, easier, and more accurate analysis tools optimized for cGMP labs

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The GS-900 Calibrated Densitometer is a self-calibrating 21 CFR Part 11 gel imaging system for acquiring gel, blot, and film images. The GS-900 Densitometer is an integral part of the Biologics Analysis Workflow, a complete, integrated solution.
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  • Description
  • Specifications
  • Ordering
  • Kits & Reagents
  • Precast Gels
  • MW Standards
  • Documents

Description

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What is the Biologics Analysis Workflow?

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The GS-900 Calibrated Densitometer is designed to reproducibly image gels, blots, and film and quantitate proteins across a wide dynamic range. The GS-900 Densitometry System uses transmissive and reflective imaging to scan chromogenic samples at the optimal detection wavelength. The three-color red, green, and blue CCD imaging technology enables accurate, reproducible quantitation with a wide variety of common stains.

To meet cGMP environment needs, the GS-900 Densitometer offers a security edition software module for 21 CFR 11 complliance and an Installation and Operations Qualification (IQ/OQ) Kit.

Applications and Uses

  • Imaging of diverse samples on 1-D and 2-D gels, colorimetric dot and slot blots, film-based chemiluminescent blots, and autoradiograms
  • Protein quantitation for Coomassie Blue- and silver-stained gels over a wide dynamic range of 0–3.4 OD
  • Scanning large gels — oversized 29 × 33 cm imaging area
  • Rapid image acquisition and analysis with intuitive, user-friendly Image Lab Software

Features and Benefits of the GS-900 Calibrated Densitometer

  • Automatic self-calibration before each run
    • The GS-900 Calibrated Densitometer uses built-in NIST-traceable step tablets for internal calibration to ensure the accuracy of each scan
    • Internal calibration can be validated with an external NIST-traceable target to confirm accuracy and reproducibility using the GS-900 Calibrated Densitometer Installation and Operations Qualification Kit (1707994)
    • One-touch operation button for further ease of image acquisition
  • Image Lab Software greatly simplifies image analysis
    • Automated analysis saves time and ensures consistency between runs
    • Includes detailed tutorials; no previous imaging experience required to produce optimum gel and blot images
    • Flexible analysis — simply modify user settings to match SOPs and fine-tune band detection, background level, and other settings
    • Easy reporting with the ability to create customized reports
  • Meets 21 CFR Part 11 regulatory requirements with an optional Image Lab Software Security Module (1709691)
  • 16-bit precision and 36.3 μm resolution for the analysis of very close bands on a gel
  • Sealed imaging area to accommodate wet samples of variable thickness

Related Products

The GS-900 Calibrated Densitometry System and Image Lab Software are part of the Biologics Analysis Workflow — an integrated suite of products designed and validated to assess the purity or identity of biological products in a cGMP environment.

The Biologics Analysis Workflow provides:

  • Increased electrophoresis throughput
  • Simplified, sensitive, and reproducible gel staining
  • Accurate and reproducible imaging
  • Easy data analysis and report generation

Specifications

Calibration frequency Automatic calibration before every scan
21 CRF Part 11 Yes (optional software package)
Acquisition software ImageLab™ software ≥5.0
Light source LED
Wavelength 400–750 nm
Absorbance range 0.0 to 3.4 OD Transmissive
0.0 to 2.7 OD RD reflective
Transmissive scan area 29 × 33 cm
Reflective scan area 29 × 33 cm
Bit depth 16-bit grayscale
Minimum pixel size 36.3 µm (700 dpi)
Image Lab™ software OS compatibility Windows XP, 7, and 10
Connection type USB 2.0
Imager dimensions (W x L x H) 62.7 × 37.6 × 18.5 cm
Imager weight 16.1 kg
Applications Densitometry
Calibrated colorimetric gel and blot quantitation

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GS900 Calibrated Densitometer

1707989
Gel densitometry system, includes scanner and cables, optional PC-compatible Image Lab™ software, 21 CFR part 11 and installation Qualification/Operations Module.

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GS-900™ Calibrated Densitometry System

1707991
Gel densitometry system, includes scanner, cables, PC-compatible Image Lab™ software, optional 21 CFR Part 11 and Installation Qualification/Operations Qualification Module

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GS-900 Calibrated Densitometer Regulatory Tools Package

1707993
Pkg of 1, set of protocols for validating the calibration of GS-900 calibrated densitometer (#170-7991), Image Lab Security Edition with 21 CFR Part 11 Module

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$9,928.00 
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GS-900™ Installation Qualification/Operations Qualification (IQ/OQ) Kit

1707994
Pkg of 1, set of protocols and one NIST-traceable external step table for validating the calibration of GS-900™ calibrated densitometer (#170-7991)

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$6,195.00 
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(Discontinued) Image Lab™ Software

1709690
Software, PC or Mac, for automated image capture, optimization, and 1-D data analysis, for use with Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ XRS+, ChemiDoc MP, and GS-900™ imaging systems

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • Image Lab Software on USB drive (12012931)
(Discontinued) Image Lab Software, Security Edition, 1 license

1709691
FDA CFR 21 Part 11 module for Image Lab Software, 1 license, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • Image Lab Software, Security Edition, 1 license (17006130)
Image Lab Software, Security Edition, 5 licenses

1709692
FDA CFR 21 Part 11 module for Image Lab Software, 5 licenses, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Image Lab Software, Security Edition, 5 licenses (17006171)
Image Lab Software, Security Edition, 10 licenses

1709693
FDA CFR 21 Part 11 module for Image Lab Software, 10 licenses, for use with ChemiDoc MP, ChemiDoc, ChemiDoc XRS+, Gel Doc EZ, Gel Doc XR+, and GS-900 Imaging Systems

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Image Lab Software, Security Edition, 10 licenses (17006172)

Kits & Reagents

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QC Colloidal Coomassie Stain

1610803
1 L, ready-to-use, non-hazardous colloidal Coomassie G-250 stain for protein polyacrylamide gels

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$100.00 
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4x Laemmli Sample Buffer

1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE

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$15.00 
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2x Laemmli Sample Buffer

1610737
30 ml, premixed protein sample buffer for SDS-PAGE

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$14.00 
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10x Tris/Glycine/SDS

1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$32.00 
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Dithiothreitol (DTT)

1610611
5 g, dithiothreitol (DTT) reducing agent

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$94.00 
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2-Mercaptoethanol

1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

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$27.00 
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Precast Gels

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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671085
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671084
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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4–15% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671083
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671094
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671093
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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12% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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12% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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12% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$15.00 
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MW Standards

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Precision Plus Protein™ All Blue Prestained Protein Standards

1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications

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$111.00 
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Precision Plus Protein™ All Blue Prestained Protein Standards, value pack

1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications

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$495.00 
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Precision Plus Protein Dual Color Standards, 500 µl

1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications

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$146.00 
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Precision Plus Protein™ Dual Color Standards, 2.5 ml

1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications

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$596.00 
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Precision Plus Protein™ Dual Xtra Prestained Protein Standards

1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications

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$157.00 
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Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack

1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications

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$647.00 
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Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards

1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications

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$157.00 
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Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack

1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

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$642.00 
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Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml

1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

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$105.00 
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Precision Plus Protein™ Unstained Protein Standards, Strep-tagged, recombinant, 5 ml

1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

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$461.00 
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Documents

Number Description Options
7250 From Discovery to Manufacturing: A Workflow for Protein-Based Immunotherapies Click to download
7051 Imaging Systems Family Flier Click to download [ Add to Cart (Free) ]
10030885 Installing your GS-900™ Calibrated Densitometer Quick Start Guide, Rev C Click to download
6421 Image Lab™ Software for the GS-900™ Densitometer Quick Start Guide, Rev A Click to download
10000076954 GS-900™ Calibrated Densitometer with Image Lab™ Software Click to download
6385 Biologics Analysis Workflow™ Brochure, Rev A Click to download [ Add to Cart (Free) ]
10033507 Biologics Analysis Workflow Product Information Sheet Click to download
4006028 Instruction Manual, Laemmli Sample Buffer, Rev J Click to download
10032602 QC Colloidal Coomassie Stain Manual, Rev A Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev C Click to download
2895 Protein Blotting Guide, Ver C Click to download
2651 2-D Electrophoresis Workflow How-To Guide, Rev F Click to download
6387 Biologics Analysis Workflow™ Model Comparability Study — Obtaining cGMP Approval for Validated SDS-PAGE Assays with the GS-900™ Calibrated Densitometer Click to download [ Add to Cart (Free) ]

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