Prestained Protein Standards

Precision Plus Protein™ standards for protein electrophoresis contain ten prestained, highly purified recombinant proteins with molecular masses from 10 to 250 kD.

Ordering Information

Overview

Precision Plus Protein™ Prestained Standards are available in Dual Color, All Blue, Kaleidoscope™, and Dual Xtra options. All four standards have the same gel migration patterns, with three high-intensity reference bands (25, 50, and 75 kD).

New and Improved Dual Color Standards:

Brighter for easier identification of target protein
Sharper for more accurate molecular weight estimation
Stronger band intensity throughout blot development

The overall brightness of Precision Plus Dual Color Standards has been increased for visual robustness throughout the entire protein electrophoresis and western blotting workflow. Improved Dual Color Standards are now brighter than the competition on both gels and blots.

Dual Xtra Standards

Dual Xtra Standards consist of 12 recombinant proteins between 2 and 250 kD; all others consist of 10 recombinant proteins between 10 and 250 kD (note that the 2 kD band is not visible on fluorescent blots).

Kaleidoscope Standards

Kaleidoscope Standards are a mixture of ten recombinant proteins in four colors (blue, pink, yellow, and green; 10–250 kD), enabling easy gel and blot orientation via the multicolor pattern.

Features and Benefits of Precision Plus Protein Prestained Standards

MW markers confirmed by mass spectrometry
Strong linear relationship between markers' log molecular weights and relative migration distances
Prestained bands for easy visual monitoring of electrophoresis
Fluorescent dyes for imaging on gels blots

Applications of Precision Plus Protein Prestained Standards

Accurate molecular weight estimation
Monitoring of electrophoresis and transfer efficiency

More Information

Fluorescence Properties of Precision Plus Protein Prestained Standards
		Fluorescent Bands
Visible Band Color	Excitation Color	All Blue	Dual Color	Dual Color Extra	Kaleidoscope
Blue	635 nm laser (Red LED)	10–250 kD	10–20, 37, 50, 100–250 kD	5–20, 37, 50, 100–250 kD	15, 20, 50, 100–250 kD
Pink	532 nm laser (Green LED)	—	25, 75 kD	25, 75 kD	25, 75 kD
Green	635 nm laser (Red LED)	—	—	—	37 kD
Yellow	488 nm laser (Blue LED)	—	—	—	10 kD

See Bulletin 5685 and Bulletin 5723 for more details on the fluorescence properties of Precision Plus Protein Prestained Standards.

Related Technologies

Related Products

Specifications

MW size range

2–250 kD

Stain type

Markers consist of highly purified recombinant proteins covalently stained with multicolored dyes

Volume

500 µl (value pack: 2,500 µl)

Total number of applications

50 (value pack: 250 applications)

Loading buffer

30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN₃

Shelf life

Store for 6 months at 4°C or –20°C until expiration date (Kaleidescope: 12 months at –20°C)

Contents

1 x 500 µl tube, instruction sheet, and standards migration sticker

Recommended loading volume

Electrophoresis: 10 μl
Blotting: 7 μl

Ordering

items

Use the filters below to refine results!

(Discontinued) Precision Plus Protein Dual Color Standards, 500 µl

1610374

500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications

(Discontinued) Precision Plus Protein™ Dual Color Standards, trial size

1610374S

50 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 5 applications

(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml

1610394

2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications

(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards

1610373

500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications

(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards, value pack

1610393

2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications

(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards

1610375

500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications

(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack

1610395

2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards

1610377

500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications

(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack

1610397

2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications

Suggested

(Discontinued) 2x Laemmli Sample Buffer

1610737

30 ml, premixed protein sample buffer for SDS-PAGE

(Discontinued) 2-Mercaptoethanol

1610710

25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

(Discontinued) XT Sample Buffer

1610791

10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels

(Discontinued) XT Reducing Agent

1610792

1 ml, 20x reducing agent for use with all Criterion XT gels

(Discontinued) Tricine Sample Buffer for Protein Gels, 30 ml

1610739

30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250

(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml

1610363

1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl

1610376

250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

(Discontinued) Precision Plus Protein™ Standard Plugs, unstained, Strep-tagged, recombinant

1610378

Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands

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