Precision Plus Protein™ Prestained Standards

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Overview

Improved Dual Color Standards Are the Brightest Overall
Improved Dual Color Standards Are the Brightest Overall

Bio-Rad's New and Improved Precision Plus Protein™ Dual Color Standards are brighter for easier identification of target proteins. Sharper bands allow for more accurate molecular weight estimation, and stronger band intensity after blot development provides visual confirmation of transfer efficiency.

Protein Standards For Electrophoresis and Western Blotting

PDF Bulletin 2998 Download the Protein Standards Application Guide

Migration Patterns (10–250 kD)

Standard protein migration on Ready Gel® Tris-HCI precast gels. The banding patterns indicate the optimal separation ranges (in kD) for each acrylamide percentage in combinations with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

Precision Plus Protein Prestained Standards are available in Dual Color, All Blue, Kaleidoscope™, and Dual Xtra options. All four standards have the same gel migration patterns, with 3 high-intensity reference bands (25, 50, and 75 kD).

New and improved Dual Color Standards:

  • Brighter for easier identification of target protein
  • Sharper for more accurate molecular weight estimation
  • Stronger band intensity throughout blot development

The overall brightness of Precision Plus Dual Color Standards has been increased for visual robustness throughout the entire protein electrophoresis and western blotting workflow. Improved Dual Color Standards are now brighter than the competition on both gels and blots.

Dual Xtra Standards

Dual Xtra Standards consist of 12 recombinant proteins between 2 and 250 kD, all others consist of 10 recombinant proteins between 10 and 250 kD (note that the 2 kD band is not visible on fluorescent blots).

Kaleidoscope Standards

Kaleidoscope Standards are a mixture of ten recombinant proteins in four colors (blue, pink, yellow, and green; 10–250 kD), enabling easy gel and blot orientation via the multicolor pattern.

Features and Benefits of Precision Plus Protein Prestained Standards

  • MW markers confirmed by mass spectrometry
  • Strong linear relationship between markers' log molecular weights and relative migration distances
  • Prestained bands for easy visual monitoring of electrophoresis
  • Fluorescent dyes for imaging on gels blots

Applications of Precision Plus Protein Prestained Standards

More Information

Fluorescence Properties of Precision Plus Protein Prestained Standards
  Fluorescent Bands
Visible Band Color Excitation Color All Blue Dual Color Dual Color Extra Kaleidoscope
Blue 635 nm laser
(Red LED)
10–250 kD 10–20, 37, 50, 100–250 kD 5–20, 37, 50, 100–250 kD 15, 20, 50, 100–250 kD
Pink 532 nm laser
(Green LED)
25, 75 kD 25, 75 kD 25, 75 kD
Green 635 nm laser
(Red LED)
37 kD
Yellow 488 nm laser
(Blue LED)
10 kD

See Bulletin 5685 and Bulletin 5723 for more details on the fluorescence properties of Precision Plus Protein Prestained Standards.

MW size range 2–250 kD
Stain type Markers consist of highly purified recombinant proteins covalently stained with multicolored dyes
Volume 500 (value pack: 2,500 µl)
Total number of applications 500 (value pack: 250 applications)
Loading buffer 30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN3
Shelf life Store for 6 months at 4°C or –20°C until expiration date (Kaleidescope: 12 months at –20°C)
Contents 1 x 500 µl tube, instruction sheet, and standards migration sticker
Recommended loading volume Electrophoresis: 10 μl
Blotting: 7 μl
Precision Plus Protein™ Dual Color Standards
Precision Plus Protein™ Dual Color Standards

161-0374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications

List Price:   $120.00
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Precision Plus Protein™ Dual Color Standards
Precision Plus Protein™ Dual Color Standards Trial Size

161-0374S
50 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 5 applications

List Price:   $24.00
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Precision Plus Protein™ Dual Color Standards Value Pack
Precision Plus Protein™ Dual Color Standards Value Pack

161-0394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications

List Price:   $510.00
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Precision Plus Protein™ All Blue Standards
Precision Plus Protein™ All Blue Standards

161-0373
500 μl, mixture of 10 blue-stained recombinant proteins (10–250 kD), including 3 reference bands (25, 50, 75 kD), 50 applications

List Price:   $97.00
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Precision Plus Protein™ All Blue Standards Value Pack
Precision Plus Protein™ All Blue Standards Value Pack

161-0393
2.5 ml (5 x 500 µl), mixture of 10 blue-stained recombinant proteins (10–250 kD), including 3 reference bands (25, 50, 75 kD), 250 applications

List Price:   $420.00
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Precision Plus Protein™ Kaleidoscope™ Standards
Precision Plus Protein™ Kaleidoscope™ Standards

161-0375
500 μl, mixture of 10 multicolor recombinant proteins (10–250 kD), 50 applications

List Price:   $130.00
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Precision Plus Protein™ Kaleidoscope™ Standards Value Pack
Precision Plus Protein™ Kaleidoscope™ Standards Value Pack

161-0395
2.5 ml (5 x 500 µl), mixture of 10 multicolor recombinant proteins (10–250 kD), 250 applications

List Price:   $545.00
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Precision Plus Protein™ Dual Xtra Standards
Precision Plus Protein™ Dual Xtra Standards

161-0377
500 μl, mixture of 12 recombinant proteins (2–250 kD), 9 blue-stained bands, and 3 pink reference bands (2, 25, 75 kD), 50 applications

List Price:   $130.00
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Precision Plus Protein™ Dual Xtra Standards Value Pack

161-0397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), 9 blue-stained bands, and 3 pink reference bands (2, 25, 75 kD), 250 applications

List Price:   $550.00
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Number Description Options
6533 Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A Click to download
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
4110025 Instruction Manual, Dual Color Precision Plus Protein Standards, Rev H Click to download
4110024 Instruction Manual, All Blue Precision Plus Protein Standards, Rev D Click to download
4110182 Instruction Manual, Precision Plus Protein Kaleidoscope Standards, Rev G Click to download
10018393 Precision Plus Protein Dual Xtra Standards Instruction Manual, Rev D Click to download
10008761 Instruction Manual, Precision Plus Protein WesternC Standards, Rev F Click to download
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]
3144 Using Precision Plus Protein Standards to Determine Molecular Weight, Rev B Click to download
3133 Molecular Weight Determination by SDS-PAGE, Rev B Click to download
2847 Strep-tag Technology for Molecular Weight (MW) Determinations on Blots Using Precision Plus Protein Standards, Rev B Click to download
3093 Precision Plus Protein Standards Competitive Comparison Card, Rev B Click to download
2800 Precision Plus Protein Dual Color Standards Competitive Card, Rev C Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
2799 Precision Plus Protein Standards: The Sharpest Standards in the Box Flier, Rev B Click to download
3118 Protein Standards for Electrophoresis and Blotting Brochure, Rev D Click to download [ Add to Cart (Free) ]
3092 Precision Plus Protein Standards Flier, Rev B Click to download
5561 Precision Plus Protein WesternC Standards, Rev E Click to download [ Add to Cart (Free) ]
5957 Precision Plus Protein Dual Xtra Standards Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]