Running Buffers and Reagents

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Overview

Premixed buffers are available for a variety of protein and nucleic acid electrophoresis protocols. The high quality 0.4 µm filtered buffers can be used with handcast or precast gels.
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Description

Description

Reduce preparation time and eliminate a source of error by using Bio-Rad’s range of premixed protein electrophoresis running buffers. There are no reagents to weigh or filter; just dilute with distilled deionized water. A wide variety of premixed Tris buffers (such as Tris/glycine/SDS, Tris/glycine, and Tris/tricine/SDS) and high-purity, electrophoresis-grade powders (such as Tris, glycine, tricine, and urea) are available for protein electrophoresis and can be used with both handcast gels and Bio-Rad’s precast gels (see the protein gel selection guide).

Features and Benefits of Running Buffers and Reagents for Protein Electrophoresis

  • Premixed buffers – all electrophoresis-purity reagents used are quality controlled for reproducible results; save preparation time while avoiding potential error due to incorrect buffer concentration
  • Variety of sizes – electrophoresis running buffers and reagents are available in packages ranging from 250 ml bottles to 5 L cubes for different throughput needs
  • Bulk 5 L boxes offer economy and convenience; the boxes feature easy-pouring spouts and are compact and stackable to save bench space

Applications and Uses of Running Buffers and Reagents for Protein Electrophoresis

Bio-Rad’s high-quality premixed electrophoresis running buffers are used for

More Information

Compare protein electrophoresis buffers to select the most appropriate buffers for your workflow.

To choose the best gel for your application, see the gel selection guide. Choices include the innovative TGX™ SDS-PAGE system with precast Mini-Protean® TGX™ and Mini-Protean® TGX Stain-Free™ Gels, and midi-sized Criterion™ TGX™ and Criterion™ TGX Stain-Free™ Gels. For hand casting, TGX™ FastCast™ and TGX Stain-Free™ FastCast™ Acrylamide Solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels.

Ordering

Ordering

items

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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610761
Pkg of 1, 250 ml, electrophoresis buffer, 7 mM phosphoric acid, for use in isoelectric focusing
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1610762
Pkg of 1, 250 ml, electrophoresis buffer, 20 mM lysine, 20 mM arginine, for use in isoelectric focusing
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1610765
Pkg of 1, 125 ml, 10x premixed electrophoresis buffer, contains 25% Triton X-100, for use with Zymogram Gels
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1610766
Pkg of 1, 10x buffer, 1x final concentration contains 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM CaCl2, 0.02% Brij-35, for use with Zymogram Gels
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1610788
Pkg of 1, 500 ml, electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels
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1610789
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels
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1610790
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Tris-Acetate Gels
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1610793
Pkg of 1, electrophoresis buffer kit, includes 500 ml XT MOPS Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml XT Reducing Agent
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(Discontinued) XT MES Buffer Kit
1610796
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT MES Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent
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1610797
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent
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(Discontinued) Glycine, 250 g
1610717
Pkg of 1, 250 g, glycine (aminoacetic acid) powder
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(Discontinued) Glycine, 1 kg
1610718
Pkg of 1, 1 kg, glycine (aminoacetic acid) powder
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(Discontinued) Glycine, 2 kg
1610724
Pkg of 1, 2 kg, glycine (aminoacetic acid) powder
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(Discontinued) Tricine
1610713
Pkg of 1, 500 g, Tricine (N-Tris[hydroxymethyl]methylglycine) powder
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(Discontinued) Tris
1610716
500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)
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(Discontinued) Tris
1610719
1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)
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(Discontinued) Urea
1610730
Pkg of 1, 250 g, urea (carbamide)
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(Discontinued) Urea
1610731
Pkg of 1, 1 kg, urea (carbamide)

Documents

Documents

Number Description Options
2317
Ready-to-Run Buffers and Solutions Brochure, Rev F